| Project/Area Number |
24592708
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
MORI Hiroki 東京医科歯科大学, 医歯(薬)学総合研究科, 講師 (80345305)
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| Co-Investigator(Kenkyū-buntansha) |
OKAZAKI Mutsumi 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (50311618)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 陰圧療法 / 脂肪再生 / 神経再生 |
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| Outline of Final Research Achievements |
The purpose of this study was to evaluate the influence of the external expander to a peripheral nerve and fat graft. A flap was made in the dorsum of the nude mouse and the expander was put on the flap. The negative pressure (-15 to -30mmHg, 10hours/day) was given via expander for 5 days. In the fat graft, the negative pressure was given 1 week before graft and 2 weeks after graft. Human fat was grafted in the dorsum of the mouse and the expander was put on the dorsum. Fat graft + negative pressure, fat graft combined with basic fibroblast growth factor (bFGF) + negative pressure, and fat graft only were compared. Adipocyte was extensively cultivated. The distal sides of the flap become necrotic due to negative pressure. Neuropeptide was increased in the 5th day. In fat graft, absorption was recognized in the negative pressure group. And survival of fat graft increased with bFGF. In extensive culture of adipocyte, number of adipocyte was decreased, but synthetic ability was increased.
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