Transformation frequency of bacteria under simulated microgravity

Research Project

Project/Area Number	24620006
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	宇宙生命学
Research Institution	Osaka University
Principal Investigator	ICHIJO TOMOAKI 大阪大学, 薬学研究科(研究院), 助教 (20513899)
Co-Investigator(Kenkyū-buntansha)	YAMAGUCHI Nobuyasu 大阪大学, 大学院薬学研究科, 准教授 (20252702)
Project Period (FY)	2012-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000) Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	宇宙生命科学 / 細菌間遺伝子伝播 / 環境微生物学
Outline of Final Research Achievements	We compared the transformation frequencies of E. coli and natural competent bacteria under normal gravity and under low-shear modeled microgravity (LSMMG) generated by a high- aspect rotating vessel (HARV). Our results demonstrated that bacterial transformation is not hampered by LSMMG, and the potential risk of bacterial gene transfer during space flight is comparable to that on Earth. Therefore, we should arrest the spread of harmful genes such as toxin-producing and antibiotic resistance genes in crewed space habitats, as well as on the Earth.

Report

(5 results)