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Utilization strategy of trace element selenium in marine microalgae

Research Project

Project/Area Number 24770117
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional biochemistry
Research InstitutionUniversity of Tsukuba

Principal Investigator

ARAIE Hiroya  筑波大学, 生命環境系, 特任助教 (30596169)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsセレン / トランスポーター / セレン含有化合物 / Emiliania huxleyi / リポソーム / 微量元素 / 輸送体 / 円石藻 / セレノシステイン
Research Abstract

There is only trace amounts of essential element selenium (Se) in environment. Therefore, microalgae thought to possess specific Se transporter for efficient utilization of Se. I tried to identify this Se transporter. To date, there are no reports of transporters specific for selenite. This time, I aimed at building activity measurement system for the identification. Considering the fact that the nucleotide sequence of the algae is GC rich and the transporter is a membrane protein, I tried expression analysis by using a cell-free system. As a result, it was possible to synthesize a membrane protein by using liposomes as basis. In addition, I have found that there may be a similarity between Se compounds in various microalgae. Therefore, it is expected to identify Se transporter that uses algae that can be genetically engineered for knockdown assay.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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