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Retinal ganglion cell protection in normal tension glaucoma model mice by anti-oxidant and anti-inframatory carotenoid.

Research Project

Project/Area Number 24791827
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Ophthalmology
Research InstitutionHokkaido University

Principal Investigator

NITTA Takuya  北海道大学, 医学(系)研究科(研究院), 客員研究員 (90507576)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords神経保護 / 網膜神経節細胞 / 緑内障 / 抗酸化物質 / 海産物 / 国際情報交換
Outline of Final Research Achievements

Purpose: To investigate the cytoprotective effect of astaxanthin (ASX) on retinal ganglion cell (RGC) degeneration using a normal tension glaucoma (NTG) mouse model which lacks glutamate/aspartate transporter (GLAST) in Muller cells.
Methods: Three-week old GLAST+/- mice were given intraperitoneal injection of ASX (60, 30, and 10 mg/kg/day, respectively) or vehicle alone, and the wide type (WT) mice were given vehicle alone for 14 days, respectively. At the 5th week, the number of RGC 4-hydroxy-2-nonenal (HNE) in retinas was analyzed with Western blotting. and NF-kB was measured using an EIA assay kit. Results and Conclusion: The number of RGC of GLAST+/- mice significantly decreased, as compared to that of WT mice. However, RGC loss was suppressed by administration of ASX at a dose dependent manner. Following ASX administration, HNE was significantly down-regulated, but NF-kB was steady. Our data suggest that ASX may be applicable for NTG as a promising therapeutics.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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