| Project/Area Number |
25410164
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Kansai University (2015)
Kanagawa Industrial Technology Center (2013-2014) |
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Principal Investigator |
ITO Takeshi 関西大学, システム理工学部, 准教授 (50426350)
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| Co-Investigator(Renkei-kenkyūsha) |
AOKI Nobuyoshi 神奈川県産業技術センター, 主任研究員 (10231763)
KANEKO Satoru 神奈川県産業技術センター, 主任研究員 (40426359)
SUZUKI Koji 慶應義塾大学, 理工学部, 教授 (80154540)
Citterio Daniel 慶應義塾大学, 理工学部, 教授 (00458952)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ストレス評価 / ＱＣＭ / 可搬型 / 短時間評価 / ストレス / コルチゾール / 水晶振動子 |
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| Outline of Final Research Achievements |
Cortisol is well known as a stress marker of endocrine system. We focused on quartz crystal microbalance (QCM) method, which is one of label free and real time measurement of antigen-antibody interaction with simplicity, convenience and low cost. No study has been reported on cortisol detection using the QCM method. In addition, we propose a QCM sensor coupled with a passive flow system for on-site monitoring of cortisol. We optimized the condition of cortisol detection such as selecting antibody and tracer, and regeneration solution. As the results, the detection time was within 8 minutes. It took 13 minutes including regeneration step. These results indicate that out proposed cortisol detection system is convenient with highly sensitivity using passive flow system.
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