Cortisol detection system for personal stress monitoring

2015 Fiscal Year Final Research Report

• Project/Area Number: 25410164
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Kansai University (2015); Kanagawa Industrial Technology Center (2013-2014)
• Principal Investigator: ITO Takeshi 関西大学, システム理工学部, 准教授 (50426350)
• Co-Investigator(Renkei-kenkyūsha): AOKI Nobuyoshi 神奈川県産業技術センター, 主任研究員 (10231763) ; KANEKO Satoru 神奈川県産業技術センター, 主任研究員 (40426359) ; SUZUKI Koji 慶應義塾大学, 理工学部, 教授 (80154540) ; Citterio Daniel 慶應義塾大学, 理工学部, 教授 (00458952)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ストレス評価 / ＱＣＭ

Outline of Final Research Achievements

Cortisol is well known as a stress marker of endocrine system. We focused on quartz crystal microbalance (QCM) method, which is one of label free and real time measurement of antigen-antibody interaction with simplicity, convenience and low cost. No study has been reported on cortisol detection using the QCM method. In addition, we propose a QCM sensor coupled with a passive flow system for on-site monitoring of cortisol. We optimized the condition of cortisol detection such as selecting antibody and tracer, and regeneration solution. As the results, the detection time was within 8 minutes. It took 13 minutes including regeneration step. These results indicate that out proposed cortisol detection system is convenient with highly sensitivity using passive flow system.

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