Molecular mechanisms for the evolution of insect prolegs
| Project/Area Number |
25440220
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | National Agriculture and Food Research Organization (2016)
National Institute of Agrobiological Sciences (2013-2015) |
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Principal Investigator |
Tomita Shuichiro 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門 新産業開拓研究領域, ユニット長 (30360457)
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| Co-Investigator(Kenkyū-buntansha) |
畠山 正統 国立研究開発法人農業生物資源研究所, その他部局等, 主任研究員 (50281142)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 連続相同 / カイコ / カブラハバチ / 腹脚 / ノックアウト / ノックダウン / RNAi / RNA-Seq / 免疫組織化学 / in situハイブリダイゼーション / 特異抗体 / RNASeq |
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| Outline of Final Research Achievements |
Proleg is abdominal appendage that develops in the larval abdomen of Lepidoptera, Mecoptera and Symphyta of Hymenoptera. The limited distribution of proleg-baring species raises the question whether prolegs are homologous structures or convergent traits. To address this issue we analyzed the molecular mechanisms underlying the proleg development. Dll expression is detected at their tips during embryonic development in Bombyx mori.
The development of appendages in abdominal segments are suppressed by the expression of Ubx, abd-A and Abd-B. Strange enough, the abd-A null mutant strain of Bombyx fail to develop prolegs . Moreover, knocking-down Dll made no effects on proleg development while gnathal appendages and thoracic legs are greatly reduced. Prolegs of Athalia rosae (Hymenoptera) are also segmented but lack crochets or any distal-most structures and no Dll expression was detected. These results suggest prolegs of Lepidoptera and Hymenoptera are of different origin.
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Report
(5 results)
Research Products
(1 results)
