Image analysis of infection-specific proteins formed in the Japanese birch plantlet infected with Inonotus obliquus

• Project/Area Number: 25450204
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Forest science
• Research Institution: Utsunomiya University
• Principal Investigator: Yokota Shinso 宇都宮大学, 農学部, 教授 (60210613)
• Co-Investigator(Kenkyū-buntansha): KAMITAKAHARA HIROSHI 京都大学, 農学研究科, 准教授 (10293911) ; YOSHINAGA ARATA 京都大学, 農学研究科, 准教授 (60273489)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: シラカンバ / カバノアナタケ / 菌感染特異的タンパク質 / MALDI/TOF/MSイメージング / 樹病 / ペルオキシダーゼ

Outline of Final Research Achievements

In the present study, distribution of fungal-infection-specific proteins and phenolic compounds, which were produced in 3-month-old Japanese birch plantlet No.8 infected with a canker-rot fungus Inonotus obliquus strain IO-U1, were investigated using matrix-assisted laser desorption/ionization/time-of-flight mass spectrometry imaging analysis. As the results, fungal-infection-specific peroxidase was found to be distributed in the whole xylem part of the cross section. In addition, the production of condensed tannin was suggested as the phenolic compound in the vicinity of the fungus-inoculated part. Moreover, actin filament bundles, one of the cytoskeleton proteins, were located nearby the fungus-inoculated part. Furthermore, 12 fungal-infection-specific proteins were newly identified.

Research Products

• [Journal Article] Proteomic analysis of responsive proteins induced in Japanese birch plantlet treated with salicylic acid (2014)
  • Author(s): Suzuki H., Takashima Y., Ishiguri F., Yoshizawa N., Yokota S.
  • Journal Title: proteomes Volume: 2 Issue: 3 Pages: 323-340
  • DOI: 10.3390/proteomes2030323
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] カバノアナタケIO-U1株に感染したシラカンバ幼植物体No.8に生成する菌感染特異的タンパク質の同定及び発現量解析 (2016)
  • Author(s): 飯塚早紀、大島潤一、石栗太、横田信三
  • Organizer: 第66回日本木材学会大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2016-03-27
• [Presentation] カバノアナタケ菌IO-U1株に感染したシラカンバ幼植物体No.8におけるフェノール性化合物及びアクチン繊維の組織内分布 (2015)
  • Author(s): 福田慎一郎、相蘇春菜、上高原浩、吉永新、大島潤一、石栗太、飯塚和也、横田信三
  • Organizer: 第33回日本植物細胞分子生物学会（東京）大会・シンポジウム
  • Place of Presentation: 東京大学農学部キャンパス
  • Year and Date: 2015-08-10
• [Presentation] Identification of specific proteins produced in each organ of Japanese birch plantlet treated with azelaic acid (2015)
  • Author(s): Ichikawa T., Ishiguri F., Iizuka K., Yokota S.
  • Organizer: International Symposium on Wood Science and Technology 2015
  • Place of Presentation: Tower Hall Funabori, Tokyo, Japan
  • Year and Date: 2015-03-15 – 2015-03-17
• [Presentation] Distribution of the specific phenolics produced in the tissue of Japanese birch plant infected with a canker-rot fungus Inonotus obliquus (2014)
  • Author(s): Miyauchi Y., Yoshinaga A., Kamitakahara H., Ishiguri F., Iizuka K., Yokota S.
  • Organizer: XXVIIth International Conference on Polyphenols & 8th Tannin Conference
  • Place of Presentation: Nagoya University, Japan
  • Year and Date: 2014-09-02 – 2014-09-06
• [Presentation] カバノアナタケ菌IO-U1株に感染したシラカンバ幼植物体No.8に発現するペルオキシダーゼ活性およびフェノール性化合物の組織化学的観察 (2014)
  • Author(s): 宮内 優、吉永 新、上高原浩、石栗 太、飯塚和也、橫田信三
  • Organizer: 第32回日本植物細胞分子生物学会（盛岡）大会・シンポジウム
  • Place of Presentation: いわて県民情報交流センター
  • Year and Date: 2014-08-21 – 2014-08-22
• [Presentation] Proteomic analysis of the specific proteins produced in each organ of Japanese birch plantlet treated with azelaic acid (2014)
  • Author(s): Ichikawa T., Ishiguri F., Iizuka K., Yokota S.
  • Organizer: XVI International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: Rhodes, Greece
  • Year and Date: 2014-07-06 – 2014-07-10
• [Presentation] カバノアナタケ菌IO-U1株に感染したシラカンバ幼植物体No.8におけるフェノール性物質の堆積およびペルオキシダーゼ活性の組織観察 (2014)
  • Author(s): 宮内 優、吉永 新、上高原浩、高島有哉、石栗 太、飯塚和也、橫田信三
  • Organizer: 平成26年度日本植物病理学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-06-02 – 2014-06-04
• [Presentation] カバノアナタケ菌IO-U1株に感染した６ヶ月生シラカンバ植物体No.8に発現するペルオキシダーゼの組織内分布 (2014)
  • Author(s): 宮内 優、吉永 新、上高原 浩、高島有哉、石栗 太、飯塚和也、横田信三
  • Organizer: 第６４回日本木材学会大会
  • Place of Presentation: 愛媛県民文化会館
• [Presentation] カバノアナタケ菌IO-U1株に感染したシラカンバ幼植物体No.8に発現するペルオキシダーゼのMALDI/TOF/MSイメージング (2013)
  • Author(s): 宮内 優、吉永 新、上高原 浩、高島有哉、石栗 太、飯塚和也、横田信三
  • Organizer: 第５８回リグニン討論会
  • Place of Presentation: サンポートホール高松
• [Book] Plant Proteomics (2015)
  • Author(s): Hiromu Suzuki, Yuya Takashima, Futoshi Ishiguri, Nobuo Yoshizawa, Shinso Yokota
  • Total Pages: 227
  • Publisher: MDPI