| Project/Area Number |
25450297
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic life science
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| Research Institution | Hokkaido University |
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Principal Investigator |
Fuda Hirotoshi 北海道大学, 保健科学研究院, 特任准教授 (60576172)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAMATSU Naoshi 北海道大学, 大学院水産科学研究院, 准教授 (10443920)
神 繁樹 北海道大学, 保健科学研究院, 助教 (60531845)
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| Co-Investigator(Renkei-kenkyūsha) |
JIN Shigeki 北海道大学, 大学院保健科学研究院, 助教 (60531845)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 抗酸化物質 / マガキ / 非アルコール性脂肪肝炎 / 酸化ストレス / 肝臓 / 脂肪肝 / 線維化 / アポトーシス / NASH / NASHモデルマウス / 培養細胞 / 予防 / DHMBA / 肝庇護 / 酸化 |
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| Outline of Final Research Achievements |
This study was undertaken to characterize the hepatoprotective potency of 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), a novel phenolic antioxidant, isolated from the Pacific oyster, using hepatoma-derived cells (C3A) cultured in the presence of pro-oxidant, as well as well-known antioxidants. We found that only DHMBA significantly protected C3A cells against cell death in a dose-dependent manner, whereas other antioxidants did not. Also our study assessed the effects of DHMBA-rich oyster extracts (DOE) in a novel non-alcoholic steatohepatitis (NASH) model mouse established by combination of a high-fat diet and oxidized low-density lipoprotein. The NASH model mouse exhibited obesity, insulin resistance, hepatic steatosis, inflammation, fibrosis, and apoptosis. These changes were significantly moderated by supplementation of DOE. From these results, DHMBA might serve as a functional food for people at elevated risk for ROS-related diseases, such as NASH.
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