Bioanalytical methods for therapeutic monoclonal antibodies using selective separation technique

• Project/Area Number: 25460040
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: University of Shizuoka
• Principal Investigator: Kenichiro Todoroki 静岡県立大学, 薬学部, 准教授 (70341451)
• Co-Investigator(Kenkyū-buntansha): Hayashi Hideki 岐阜薬科大学, 薬学部, 准教授 (00419665)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 抗体医薬 / HPLC分析 / 血中薬物濃度分析 / LC/MS/MS

Outline of Final Research Achievements

In this study, we developed a simple, sensitive, accurate, and rapid analytical method for detecting therapeutic monoclonal antibodies in plasma samples using immunoaffinity purification e HT-RPLC with fluorescence detection. We successfully applied both methods to the bioanalyses of plasma samples obtained from cancer and RA patients who were administered each drug. The methods described here are applicable for planning
optimal therapeutic programs, and also for the evaluation of biological equivalencies in the development of biosimilars.

Research Products

• [Journal Article] Bioanalysis of bevacizumab and infliximab by high-temperature reversed-phase liquid chromatography with fluorescence detection after immunoaffinity magnetic purification (2016)
  • Author(s): K. Todoroki, T. Nakano, Y. Eda, K. Ohyama, H. Hayashi , D. Tsuji, J.Z. Min, K. Inoue, N. Iwamoto, A. Kawakami, Y. Ueki, K. Itoh, T. Toyo'oka
  • Journal Title: Anal. Chim. Acta Volume: 916 Pages: 112-119
  • DOI: 10.1016/j.aca.2016.02.029
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The Creation of Useful Analytical Tools to Elucidate Biological Phenomena (2015)
  • Author(s): N. Kishikawa, K. Todoroki
  • Journal Title: YAKUGAKU ZASSHI Volume: 135 Issue: 2 Pages: 189-190
  • DOI: 10.1248/yakushi.14-00213-F
  • NAID: 130004756501
  • ISSN: 0031-6903, 1347-5231
  • Year and Date: 2015-02-01
  • Peer Reviewed / Open Access
• [Journal Article] Development of HPLC Analysis Methods for Therapeutic Monoclonal Antibodies (2015)
  • Author(s): K. Todoroki
  • Journal Title: YAKUGAKU ZASSHI Volume: 135 Issue: 2 Pages: 213-218
  • DOI: 10.1248/yakushi.14-00213-4
  • NAID: 130004756498
  • ISSN: 0031-6903, 1347-5231
  • Year and Date: 2015-02-01
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Advanced dress-up chiral columns: New removable chiral stationary phases for enantioseparation of chiral carboxylic acids (2015)
  • Author(s): K. Todoroki, Y. Ishii , T. Ide, J. Z. Min, K. Inoue, X. Huang, W. Zhang , Y. Hamashima, T. Toyo’oka
  • Journal Title: Anal. Chim. Acta Volume: 882 Pages: 101-111
  • DOI: 10.1016/j.aca.2015.03.037
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] An easy-to-use excimer fluorescence derivatization reagent, 2-chloro-4-methoxy-6-(4-(pyren-4-yl)butoxy)-1,3,5-triazine, for use in the highly sensitive and selective liquid chromatography analysis of histamine in Japanese soy sauces (2015)
  • Author(s): T. Nakano, K. Todoroki, Y. Ishii, C. Miyauchi, A. Palee, J.Z. Min, K. Inoue, K. Suzuki, T. Toyo’oka
  • Journal Title: Anal. Chim. Acta Volume: 880 Pages: 145-151
  • DOI: 10.1016/j.aca.2015.04.059
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Automatic analyzer for highly polar carboxylic acids based on fluorescence derivatization–liquid chromatography. (2015)
  • Author(s): K. Todoroki, T. Nakano, Y. Ishii, K. Goto, R. Tomita, T. Fujioka, J.Z. Min, K. Inoue, T. Toyo’oka
  • Journal Title: Biomed.Chromatogr. Volume: 29 Issue: 3 Pages: 445-451
  • DOI: 10.1002/bmc.3295
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A novel approach for LC-MS/MS-based chiral metabolomics fingerprinting and chiral metabolomics extraction using a pair of enantiomers of chiral derivatization reagents (2015)
  • Author(s): Takahiro Takayama, Toshiki Mochizuki, Kenichiro Todoroki, Jun Zhe Min,Hajime Mizuno, Koichi Inoue, Hiroyasu Akatsu, Ichiro Noge, Toshimasa Toyo'oka
  • Journal Title: Analytica Chimica Acta Volume: 898 Pages: 73-84
  • DOI: 10.1016/j.aca.2015.10.010
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Metabolomics approach of infant formula for the evaluation of contamination and degradation using hydrophilic interaction liquid chromatography coupled with mass spectrometry. (2015)
  • Author(s): Inoue K, Tanada C, Sakamoto T, Tsutsui H, Akiba T, Min JZ, Todoroki K, Yamano Y, Toyo'oka T.
  • Journal Title: Food Chem Volume: 181 Pages: 318-324
  • DOI: 10.1016/j.foodchem.2015.02.117
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Towards the chiral metabolomics: Liquid chromatography-mass spectrometry based DL-amino acid analysis after labeling with a new chiral reagent, (S)-2,5-dioxopyrrolidin-1-yl-1-(4,6-dimethoxy-1,3,5-triazin-2-yl)pyrrolidine-2-carboxylate, and the application to saliva of healthy volunteers (2015)
  • Author(s): Toshiki Mochizuki, Takahiro Takayama, Kenichiro Todoroki, Koichi Inoue, Jun Zhe Min, Toshimasa Toyo'oka
  • Journal Title: Analytica Chimica Acta Volume: 875 Pages: 73-82
  • DOI: 10.1016/j.aca.2015.02.054
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Profiling of chiral and achiral carboxylic acid metabolomics: Synthesis and evaluation of triazine-type chiral derivatization reagents for carboxylic acids by LC-ESI-MS/MS and the application to saliva of healthy volunteers and diabetic patients (2015)
  • Author(s): Takahiro Takayama, Tomohiro Kuwabara, Toshio Maeda, Ichiro Noge, Yutaka Kitagawa, Koichi Inoue, Kenichiro Todoroki, Jun Zhe Min, Toshimasa Toyo'oka
  • Journal Title: Analytical and Bioanalytical Chemistry Volume: 407 Issue: 3 Pages: 1003-1014
  • DOI: 10.1007/s00216-014-8275-9
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Evaluation of a Novel Positively-Charged Pyrrolidine-Based Chiral Derivatization Reagent for the Enantioseparation of Carboxylic Acids by LC-ESI-MS/MS (2015)
  • Author(s): K. Uno, T. Takayama, K. Tosoroki, K. Inoue, J.Z. Min, H. Mizuno, T. Toyo’oka
  • Journal Title: CHROMATOGRAPHY Volume: 36 Issue: 2 Pages: 57-60
  • DOI: 10.15583/jpchrom.2015.023
  • NAID: 130005098793
  • ISSN: 1342-8284, 1348-3315
  • Peer Reviewed
• [Journal Article] First observation of N-acetyl leucine and N-acetyl isoleucine in diabetic patient hair and quantitative analysis by UPLC-ESI-MS/MS (2015)
  • Author(s): Jun Zhe Min, Yuki Tomiyasu, Takashi Morotomi, Ying-Zi Jiang, Gao Li, Qing Shi, Hai-fu Yu, Koichi Inoue, Kenichiro Todoroki, Toshimasa Toyo'oka
  • Journal Title: Clinica Chimica Acta Volume: 444 Pages: 143-148
  • DOI: 10.1016/j.cca.2015.02.006
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Human nails metabolite analysis: A rapid and simple method for quantification of uric acid in human fingernail by high-performanceliquid chromatography with UV-detection (2015)
  • Author(s): Xi-Ling Li, Gao Li, Ying-Zi Jiang, Dongzhou Kang, Cheng Hua Jin, Qing Shi,Toufeng Jin, Koichi Inoue, Kenichiro Todoroki, Toshimasa Toyo'oka, Jun Zhe Min
  • Journal Title: Journal of Chromatography B Volume: 1002 Pages: 394-398
  • DOI: 10.1016/j.jchromb.2015.08.044
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride as an enantioseparation enhancer for fluorescence chiral derivatization–liquid chromatographic analysis of DL-lactic acid. (2014)
  • Author(s): K. Todoroki, K. Goto, T. Nakano, Y. Ishii, J.-Z. Min, K. Inoue, T. Toyo'oka
  • Journal Title: J. Chromatogr. A Volume: 1360 Pages: 188-195
  • DOI: 10.1016/j.chroma.2014.07.077
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Simple and sensitive analysis of histamine and tyramine in Japanese soy sauces and their intermediates using the stable isotope dilution HILIC–MS/MS method. (2014)
  • Author(s): Todoroki, K., Ishii, Y., Miyauchi, C., Kitagawa, S., Min, J. Z., Inoue, K., Yamanaka, T., Suzuki, K., Yoshikawa, Y., Ohashi, N. and Toyo'Oka, T.
  • Journal Title: Journal of Agricultual Food Chemistry Volume: 62 Issue: 26 Pages: 6206-6211
  • DOI: 10.1021/jf500767p
  • Peer Reviewed
• [Journal Article] Computational Prediction of Diastereomeric Separation Behavior of Fluorescent <i>o</i>-Phthalaldehyde Derivatives of Amino Acids (2014)
  • Author(s): K. Todoroki, T. Nakano, H. Watanabe, J.-Z. Min, K. Inoue, Y. Ishikawa, T. Toyo'oka
  • Journal Title: Analytical Sciences Volume: 30 Issue: 9 Pages: 865-870
  • DOI: 10.2116/analsci.30.865
  • NAID: 130004687412
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Assessment of the Efficacy of Anticancer Drugs by Amino Acid Metabolomics Using Fluorescence Derivatization-HPLC (2014)
  • Author(s): Ryoko Tomita, Kenichiro Todoroki, Kazuyuki Machida, Sho Nishida, Hiroshi Maruoka, Hideyuki Yoshida, Toshihiro Fujioka, Manabu Nakashima, Masatoshi Yamaguchi, Hitoshi Nohta
  • Journal Title: Analytical Sciences Volume: 30 Issue: 7 Pages: 751-758
  • DOI: 10.2116/analsci.30.751
  • NAID: 130004442316
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed / Open Access
• [Journal Article] Determination of dicyandiamide in infant formula by stable isotope dilution hydrophilic interaction liquid chromatography with tandem mass spectrometry. (2014)
  • Author(s): K. Inoue, T. Sakamoto, J.-Z. Min, K. Todoroki, T. Toyo'oka
  • Journal Title: Food Chem. Volume: 156 Pages: 390-393
  • DOI: 10.1016/j.foodchem.2014.01.124
  • Peer Reviewed
• [Journal Article] Simultaneous microdetermination of bosentan, ambrisentan, sildenafil, and tadalafil in plasma using liquid chromatography/tandem mass spectrometry for pediatric patients with pulmonary arterial hypertension. (2014)
  • Author(s): Y. Yokoyama, M. Tomatsuri, H. Hayashi, K. Hirai, Y. Ono, Y. Yamada, K. Todoroki, T. Toyo’oka, H. Yamada, K. Itoh
  • Journal Title: J. Pharm. Biomed. Anal. Volume: 89 Pages: 227-232
  • DOI: 10.1016/j.jpba.2013.11.007
  • Peer Reviewed
• [Journal Article] Determination of acetone in saliva by reversed-phase liquid chromatography with fluorescence detection and the monitoring of diabetes mellitus patients with ketoacidosis. (2014)
  • Author(s): S. Fujii, T. Maeda, I. Noge, Y. Kitagawa, K. Todoroki, K. Inoue, J.-Z. Min, T. Toyo'oka
  • Journal Title: Clin. Chim. Acta Volume: 430 Pages: 140-144
  • DOI: 10.1016/j.cca.2014.01.006
  • Peer Reviewed
• [Journal Article] Isotopic variants of light and heavy L-pyroglutamic acid succinimidyl esters as the derivatization reagents for DL-amino acid chiral metabolomics identification by liquid chromatography and electrospray ionization mass spectrometry (2014)
  • Author(s): Toshiki Mochizuki, Kenichiro Todoroki, Koichi Inoue, Jun Zhe Min, Toshimasa Toyo'oka
  • Journal Title: Analytica Chimica Acta Volume: 811 Pages: 51-59
  • DOI: 10.1016/j.aca.2013.12.016
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Evaluation of a series of prolylamidepyridine as the chiral derivatization reagents for enantioseparation of carboxylic acids by LC-ESI-MS/MS and the application to human saliva (2014)
  • Author(s): Tomohiro Kuwabara, Takahiro Takayama, Kenichiro Todoroki, Koichi Inoue, Jun Zhe Min, Toshimasa Toyo'oka
  • Journal Title: Analytical and Bioanalytical Chemistry Volume: 406 Issue: 11 Pages: 2641-2649
  • DOI: 10.1007/s00216-014-7637-7
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A quantitative analysis of the polyamine in lung cancer patient fingernails by LC-ESI-MS/MS. (2014)
  • Author(s): J.-Z. Min, A. Matsumoto, G. Li, Y.-Z. Jiang, H.-F. Yu, K. Todoroki, K. Inoue, T. Toyo'oka
  • Journal Title: Biomed. Chromatogr. Volume: 28 Issue: 4 Pages: 492-499
  • DOI: 10.1002/bmc.3059
  • Peer Reviewed / Open Access
• [Journal Article] UPLC/ESI-MS/MS-based determination of metabolism of several new illicit drugs, ADB-FUBINACA, AB-FUBINACA, AB-PINACA, QUPIC, 5F-QUPIC and α-PVT, by human liver microsome. (2014)
  • Author(s): T. Takayama, M. Suzuki, K. Todoroki, K. Inoue, J.-Z. Min, R. Kikura-Hanajiri, Y. Goda, T. Toyo'oka
  • Journal Title: Biomed. Chromatogr. Volume: 28 Issue: 6 Pages: 831-838
  • DOI: 10.1002/bmc.3155
  • Peer Reviewed
• [Journal Article] In vivo imaging of reactive oxygen species in mouse brain by using [3H]Hydromethidine as a potential radical trapping radiotracer. (2014)
  • Author(s): K. Abe, N. Takai, K. Fukumoto, N. Imamoto, M. Tonomura, M. Ito, N. Kanegawa, K. Sakai, K. Morimoto, K. Todoroki, O. Inoue
  • Journal Title: J. Cereb. Blood Flow Metab. Volume: 34 Issue: 12 Pages: 1907-1913
  • DOI: 10.1038/jcbfm.2014.160
  • Peer Reviewed
• [Journal Article] Liquid Chromatography-tandem Mass Spectrometry with Fluorous Derivatization Method for Selective Analysis of Sialyl Oligosaccharides (2014)
  • Author(s): Yohei Sakaguchi, Tadashi Hayama, Hideyuki Yoshida, Miki Itoyama, Kenichiro Todoroki, Masatoshi Yamaguchi, Hitoshi Nohta
  • Journal Title: Rapid Communication in Mass Spectrometry Volume: 28 (23) Issue: 23 Pages: 2481-2489
  • DOI: 10.1002/rcm.7042
  • Peer Reviewed
• [Journal Article] Rapid and sensitive determination of diacetylpolyamines in human fingernail by ultraperformance liquid chromatography coupled with electrospray ionization tandem mass spectrometry. (2014)
  • Author(s): J.-Z. Min, Y. Morota, Y.-Z. Jiang, G. Li, D. Kang, H.-F. Yu, K. Inoue, K. Todoroki, T. Toyo'oka
  • Journal Title: Eur. J. Mass Spectrom. Volume: 20 Issue: 6 Pages: 477-486
  • DOI: 10.1255/ejms.1301
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of gardenia yellow using crocetin from alkaline hydrolysis based on ultra high performance liquid chromatography and high-speed countercurrent. (2014)
  • Author(s): K. Inoue, C. Tanada, H. Nishikawa, S. Matsuda, A. Tada, Y. Ito, J.-Z. Min, K. Todoroki, N. Sugimoto, T. Toyo'oka, H. Akiyama
  • Journal Title: J. Sep. Sci. Volume: 37 Issue: 24 Pages: 3619-3624
  • DOI: 10.1002/jssc.201400793
  • Peer Reviewed
• [Journal Article] Dress-Up Chiral Columns for the Enantioseparation of Amino Acids Based on Fluorous Separation (2013)
  • Author(s): Todoroki, Kenichiro; Ishii, Yasuhiro; Toyoda, Koji; Ikawa, Takashi; Min, Jun Zhe; Inoue, Koichi; Akai, Shuji; Toyo’oka, Toshimasa
  • Journal Title: Anal. Bioanal. Chem. Volume: - Issue: 25 Pages: 8121-8129
  • DOI: 10.1007/s00216-013-7207-4
  • Peer Reviewed
• [Journal Article] Fully automated reagent peak-free liquid chromatography fluorescence analysis of highly polar carboxylic acids using a column-switching system and fluorous scavenging derivatization (2013)
  • Author(s): K. Todoroki, H. Hashimoto, K. Machida, M. Itoyama, T. Hayama, H. Yoshida, H. Nohta, M. Nakashima, M. Yamaguchi
  • Journal Title: Journal of Separation science Volume: 36 Issue: 2 Pages: 232-238
  • DOI: 10.1002/jssc.201200692
  • Peer Reviewed
• [Journal Article] Development of a stable isotope dilution UPLC-MS/MS method for quantification of dexmedetomidine in a small amount of human plasma (2013)
  • Author(s): Inoue K, Sakamoto T, Fujita Y, Yoshizawa S, Tomita M, Min JZ, Todoroki K, Sobue K, Toyo'oka T
  • Journal Title: Biomed Chromatogr Volume: 27 Issue: 7 Pages: 853-8
  • DOI: 10.1002/bmc.2870
  • Peer Reviewed
• [Journal Article] High-throughput LC-MS/MS based simultaneous determination of polyamines including N-acetvlated forms in human saliva and the diagnostic approach (2013)
  • Author(s): Tsutsui H, Mochizuki T, Inoue K, Toyama T. Yoshimoto N. Endo Y.
  • Journal Title: analytical chemistry Volume: 85 Issue: 24 Pages: 11835-11842
  • DOI: 10.1021/ac402526c
  • Peer Reviewed
• [Journal Article] A novel derivatization reagent possessing a bromoquinolinium structure for biological carboxylic acids in HPLC-ESI-MS/MS (2013)
  • Author(s): Yuko Mochizuki, Shinsuke Inagaki, Jun Zhe Min, Koichi Inoue, Kenichiro Todoroki, Toshimasa Toyo’oka
  • Journal Title: Journal of Separation Science Volume: 36 Issue: 12 Pages: 1883-1889
  • DOI: 10.1002/jssc.201300083
  • Peer Reviewed
• [Journal Article] Novel chiral derivatization reagents possessing a pyridyl thiourea structure for enantiospecific determination of amines and carboxylic (2013)
  • Author(s): Nagao R, Tsutsui H, Mochizuki T, Takayama T, Kuwabara T, Min JZ
  • Journal Title: Journal of Chromatography A Volume: 1,296 Pages: 111-118
  • DOI: 10.1016/j.chroma.2013.03.019
  • Peer Reviewed
• [Journal Article] Relative quantification of enantiomers of chiral amines by high-throughout LC-ESI-MS/MS using isotopic variants of light and heavy L- (2013)
  • Author(s): Mochizuki T, Taniguchi S, Tsutsui H, Min TZ, Inoue K
  • Journal Title: Analytica Chimica Acta Volume: 773 Pages: 76-82
  • DOI: 10.1016/j.aca.2013.02.026
  • Peer Reviewed
• [Presentation] 抗体医薬のイムノアフィニティー精製-高温逆相LC分析の高感度化を実現する各種蛍光標識法の検討 (2015)
  • Author(s): 中野達基、江田康拓、轟木堅一郎、水野 初、閔 俊哲、豊岡利正
  • Organizer: 新アミノ酸分析研究会第5回学術講演会
  • Place of Presentation: 東京大学武田先端知ビル
  • Year and Date: 2015-12-07
• [Presentation] フルオラス科学を応用した生体成分分析 (2015)
  • Author(s): 轟木堅一郎
  • Organizer: 第9回日本化学会東海支部若手研究者フォーラム～分析化学の最前線～
  • Place of Presentation: 愛知工業大学
  • Year and Date: 2015-12-03
  • Invited
• [Presentation] 抗体医薬の新規血中薬物濃度分析法の開発研究 (2015)
  • Author(s): 轟木堅一郎
  • Organizer: 日本薬物動態学会第30回年会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2015-11-14
  • Invited
• [Presentation] LCを基盤とした抗体医薬の血中薬物濃度分析法の開発 (2015)
  • Author(s): 轟木堅一郎
  • Organizer: 第26回クロマトグラフィー科学会議
  • Place of Presentation: 九州大学医系キャンパス
  • Year and Date: 2015-11-13
  • Invited
• [Presentation] イムノアフィニティー精製-高温逆相LCにおける抗体医薬の高感度検出化に関する検討 (2015)
  • Author(s): 中野達基、江田康拓、轟木堅一郎、水野 初、閔 俊哲、豊岡利正
  • Organizer: 第26回クロマトグラフィー科学会議
  • Place of Presentation: 九州大学医系キャンパス
  • Year and Date: 2015-11-12
• [Presentation] Detection of anti-VEGF antibody bevacizumab using DNA aptamer. (2015)
  • Author(s): T. Saito, K. Abe, Y. Yamamoto, N. Savory, K. Todoroki, K. Ikebukuro
  • Organizer: The 42nd International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: I-messae hall, Himeji
  • Year and Date: 2015-09-23
  • Int'l Joint Research
• [Presentation] Bioanalysis for therapeutic monoclonal antibodies using affinity purification-high temperature reversed phase chromatography. (2015)
  • Author(s): T. Nakano, Y. Eda, K. Todoroki, H. Mizuno, J.Z. Min, K. Inoue, T. Toyo’oka
  • Organizer: RSC International Conference 2015
  • Place of Presentation: 幕張メッセ国際会議場
  • Year and Date: 2015-09-03
  • Int'l Joint Research
• [Presentation] 抗体医薬品の血中分析法について (2015)
  • Author(s): 轟木堅一郎
  • Organizer: 島津バイオ医薬セミナー2015
  • Place of Presentation: 島津製作所東京支社
  • Year and Date: 2015-05-26
  • Invited
• [Presentation] 再現性の高い固定相の着脱を可能とするドレスアップキラルカラムの開発 (2014)
  • Author(s): 石井裕大、轟木堅一郎、井出貴文、閔 俊哲、井之上浩一、濱島義隆、豊岡利正
  • Organizer: 第25回クロマトグラフィー科学会議（京都）
  • Place of Presentation: 京都大学船井哲良記念講堂
  • Year and Date: 2014-12-12
• [Presentation] イムノアフィニティー磁気ビーズと高温逆相LCを組み合わせた抗体医薬の血中薬物濃度分析 (2014)
  • Author(s): 中野達基、江田康拓、轟木堅一郎、閔 俊哲、井之上浩一、豊岡利正
  • Organizer: 第25回クロマトグラフィー科学会議（京都）
  • Place of Presentation: 京都大学船井哲良記念講堂
  • Year and Date: 2014-12-12
• [Presentation] イムノアフィニティー精製-高温逆相LCによる抗体医薬品の血中薬物濃度分析法の開発 (2014)
  • Author(s): 江田康拓、中野達基、轟木堅一郎、閔 俊哲、井之上浩一、豊岡利正
  • Organizer: 新アミノ酸分析研究会第4回学術講演会（東京）
  • Place of Presentation: 東京大学武田先端知ビル
  • Year and Date: 2014-11-17
• [Presentation] トリアジン構造を光学分離向上剤として用いるアミノ酸のHPLC分離分析法の開発 (2014)
  • Author(s): 中村美鈴、佐藤雄飛、後藤かの子、中野達基、轟木堅一郎、閔 俊哲、井之上浩一、豊岡利正
  • Organizer: 新アミノ酸分析研究会第4回学術講演会（東京）
  • Place of Presentation: 東京大学武田先端知ビル
  • Year and Date: 2014-11-17
• [Presentation] 遠隔位不斉識別試薬を用いるアミノ酸およびペプチド類のHPLC光学分離 (2014)
  • Author(s): 佐藤雄飛、中野達基、轟木堅一郎、井口和明、閔 俊哲、井之上浩一、武田厚司、豊岡利正：
  • Organizer: 新アミノ酸分析研究会第4回学術講演会（東京）
  • Place of Presentation: 東京大学武田先端知ビル
  • Year and Date: 2014-11-17
• [Presentation] イムノアフィニティー精製-高温逆相LCによる抗体医薬品の血漿中薬物濃度分析法の開発 (2014)
  • Author(s): 江田康拓、中野達基、轟木堅一郎、閔 俊哲、井之上浩一、豊岡利正
  • Organizer: 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2014
  • Place of Presentation: 静岡県立大学
  • Year and Date: 2014-11-09
• [Presentation] 遠隔位不斉識別試薬を用いるアミノ酸およびペプチド類のHPLC光学分離分析 (2014)
  • Author(s): 佐藤雄飛、中野達基、轟木堅一郎、井口和明、閔 俊哲、井之上浩一、武田厚司、豊岡利正
  • Organizer: 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2014
  • Place of Presentation: 静岡県立大学
  • Year and Date: 2014-11-09
• [Presentation] Simple and sensitive analysis of histamine and tyramine in Japanese soy sauces and their intermediates using the stable isotope dilution HILIC–MS/MS method. (2014)
  • Author(s): Yasuhiro Ishii, Kenichiro Todoroki, Chiemi Miyauchi, Sachiyo Kitagawa, Jun Zhe Min, Koichi Inoue, Kuniaki Suzuki, Yuko Yoshikawa, Norio Ohashi, Toshimasa Toyo’oka
  • Organizer: 第19回静岡健康・長寿学術フォーラム（沼津）
  • Place of Presentation: ふじのくに千本松フォーラム
  • Year and Date: 2014-11-07
• [Presentation] 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride as an enantioseparation enhancer for fluorescence chiral derivatization - Liquid Chromatographic analysis of DL-lactic acid. (2014)
  • Author(s): Kanoko Goto, Tatsuki Nakano, Yasuhiro Ishii, Kenichiro Todoroki, Jun Zhe Min, Koichi Inoue, Toshimasa Toyo’oka
  • Organizer: 第19回静岡健康・長寿学術フォーラム（沼津）
  • Place of Presentation: ふじのくに千本松フォーラム
  • Year and Date: 2014-11-07
• [Presentation] 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride as an enantioseparation enhancer for fluorescence chiral derivatization - Liquid Chromatographic analysis of DL-lactic acid. (2014)
  • Author(s): Kenichiro Todoroki, Kanoko Goto, Tatsuki Nakano, Yasuhiro Ishii, Jun Zhe Min, Koichi Inoue, Toshimasa Toyo’oka
  • Organizer: 11th Japan-China International Symposium on Health Sciences (Shizuoka)
  • Place of Presentation: 静岡県立大学
  • Year and Date: 2014-11-06
• [Presentation] フルオラス分離技術を基盤とする新規分離デバイスDress-upキラルカラムの開発とカルボン酸分離への応用 (2014)
  • Author(s): 石井裕大、轟木堅一郎、井出貴文、閔 俊哲、井之上浩一、濱島義隆、豊岡利正
  • Organizer: フルオラス科学研究会第7回シンポジウム（札幌）
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09
• [Presentation] 選択的な捕集と検出を可能とするフルオラスプロテオミクス解析試薬の開発 (2014)
  • Author(s): 石井裕大、轟木堅一郎、閔 俊哲、井之上浩一、豊岡利正
  • Organizer: フルオラス科学研究会第7回シンポジウム（札幌）
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09
• [Presentation] Dress-up chiral column: a new separation device for chiral compounds. (2014)
  • Author(s): Yasuhiro Ishii, Kenichiro Todoroki, Takafumi Ide, Jun Zhe Min, Koichi Inoue, Yoshitaka Hamashima, Toshimasa Toyo’oka
  • Organizer: RSC International Conference 2014
  • Place of Presentation: 幕張メッセ国際会議場
  • Year and Date: 2014-09-05
• [Presentation] 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride as an enantioseparation enhancer for fluorescence chiral derivatization - liquid chromatographic analysis of DL-lactic acid. (2014)
  • Author(s): Kanoko Goto, Tatsuki Nakano, Yasuhiro Ishii, Kenichiro Todoroki, Jun Zhe Min, Koichi Inoue, Toshimasa Toyo’oka
  • Organizer: RSC International Conference 2014
  • Place of Presentation: 幕張メッセ国際会議場
  • Year and Date: 2014-09-04
• [Presentation] キラル化合物の新規分離デバイス Dress-upキラルカラムのカルボン酸分離への応用 (2014)
  • Author(s): 石井裕大、轟木堅一郎、井出貴文、閔 俊哲、井之上浩一、濱島義隆、豊岡利正
  • Organizer: 第12回次世代を担う若手のためのフィジカル・ファーマフォーラム2014（箱根)
  • Place of Presentation: 箱根高原ホテル
  • Year and Date: 2014-07-15
• [Presentation] イムノアフィニティー精製-高温逆相LCによる抗体医薬品の血漿中薬物濃度分析法の開発 (2014)
  • Author(s): 中野達基、江田康拓、轟木堅一郎、閔 俊哲、井之上浩一、豊岡利正
  • Organizer: 第12回次世代を担う若手のためのフィジカル・ファーマフォーラム2014（箱根)
  • Place of Presentation: 箱根高原ホテル
  • Year and Date: 2014-07-14
• [Presentation] LCMS-8040を用いた医薬品および食品関連物質の簡便かつ高感度な分析法の開発 (2014)
  • Author(s): 轟木堅一郎
  • Organizer: 島津食品分析セミナーin静岡（静岡）
  • Place of Presentation: 静岡駅パルシェ会議室
  • Year and Date: 2014-07-08
  • Invited
• [Presentation] DMT-MMを光学分離向上剤として用いるアミノ酸のHPLC分離分析法の開発 (2014)
  • Author(s): 中村美鈴、佐藤雄飛、後藤かの子、中野達基、轟木堅一郎、閔 俊哲、井之上浩一、豊岡利正
  • Organizer: 第60回日本薬学会東海支部大会（鈴鹿）
  • Place of Presentation: 鈴鹿医療科学大学薬学部
  • Year and Date: 2014-07-05
• [Presentation] 遠隔位不斉識別試薬を用いるアミノ酸およびペプチド類のHPLC光学分離分析法の開発 (2014)
  • Author(s): 佐藤雄飛、中村美鈴、中野達基、後藤かの子、轟木堅一郎、閔 俊哲、井之上浩一、豊岡利正
  • Organizer: 第60回日本薬学会東海支部大会（鈴鹿）
  • Place of Presentation: 鈴鹿医療科学大学薬学部
  • Year and Date: 2014-07-05
• [Presentation] カルボン酸光学分離用Cinchona alkaloid型dress-upキラルカラムの開発 (2014)
  • Author(s): 石井裕大、轟木堅一郎、井出貴文、閔 俊哲、井之上浩一、濱島義隆、豊岡利正
  • Organizer: 第21回クロマトグラフィーシンポジウム（名古屋）
  • Place of Presentation: 名古屋市工業研究所
  • Year and Date: 2014-06-06
• [Presentation] 量子化学計算に基づくアミノ酸ジアステレオマーの光学分離メカニズムの考察 (2014)
  • Author(s): 中野 達基、轟木 堅一郎、石川 吉伸、閔 俊哲、井之上 浩一、豊岡 利正
  • Organizer: 第74回分析化学討論会（郡山）
  • Place of Presentation: 日本大学郡山キャンパス
  • Year and Date: 2014-05-24
• [Presentation] フルオラス分離技術を利用したdress-upキラルカラムの開発とアミノ酸光学分離への適用 (2013)
  • Author(s): 石井裕大、轟木堅一郎、豊田耕司、閔 俊哲、井之上浩一、豊岡利正
  • Organizer: 第20回クロマトグラフィーシンポジウム
  • Place of Presentation: 神戸大学
• [Presentation] 抗イディオタイプリコンビナントFabを用いた生物学的製剤の血中濃度測定法の構築 (2013)
  • Author(s): 大鹿雄美、林 秀樹、轟木堅一郎、坪井声示、松山耐至、辻 大樹、井上和幸、豊岡利正、山田 浩、伊藤邦彦
  • Organizer: 第59回 日本薬学会東海支部総会・大会
  • Place of Presentation: 名城大学薬学部
• [Presentation] トリプシン消化LC/MS/MSによる抗体医薬品の血漿薬物濃度分析法の開発 (2013)
  • Author(s): 豊田耕司、轟木堅一郎、林 秀樹、坪井声示、松山耐至、閔 俊哲、井之上浩一、伊藤邦彦、豊岡利正
  • Organizer: 第24回クロマトグラフィー科学会議
  • Place of Presentation: 東京大学
• [Presentation] トリプシン消化LC/MS/MSによる血漿中抗体医薬品の分析 (2013)
  • Author(s): 豊田耕司、轟木堅一郎、林 秀樹、坪井声示、松山耐至、閔 俊哲、井之上浩一、伊藤邦彦、豊岡利正
  • Organizer: 新アミノ酸分析研究会 第3回学術講演会
  • Place of Presentation: 東京大学
• [Presentation] Dress-Up Chiral Columns for the Enantioseparation of Amino Acids Based on Fluorous Separation (2013)
  • Author(s): Kenichiro Todoroki, Yasuhiro Ishii, Koji Toyoda, Jun Zhe Min, Koichi Inoue, Shuji Akai, Toshimasa Toyo'oka
  • Organizer: The 39th International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: Amsterdam
• [Presentation] Dress-up chiral columns for the enantioseparation of amino acids based on fluorous separation. (2013)
  • Author(s): Yasuhiro Ishii, Kenichiro Todoroki, Koji Toyoda, Jun Zhe Min, Koichi Inoue, Toshimasa Toyo’oka
  • Organizer: RSC International Conference 2013
  • Place of Presentation: 幕張メッセ
• [Presentation] Separation behavior characterization of therapeutic monoclonal antibody preparations by high-temperature reversed-phase LC using wide pore core-shell column. (2013)
  • Author(s): Kenichiro Todoroki, Atsuko Nakane, Koji Toyoda, Yuki Sato, Hideki Hayashi, Tsutomu Kai, Jun Zhe Min, Koichi Inoue, Shinobu Kudoh, Kunihiko Itoh, Toshimasa Toyo’oka:
  • Organizer: The 40th International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: Tasmania
• [Presentation] High Sensitivity Glycan Profiling in Antibody Drugs Using the RF-20Axs Fluorescence Detector (2013)
  • Author(s): 64. Kenichiro Tanaka, William Hedgepeth, Ayako Nomura, Tadayuki Yamaguchi, Yuki Sato, Kenichiro Todoroki
  • Organizer: Eastern Analytical Symposium & Exposition
  • Place of Presentation: New Jersey, USA
• [Remarks] 静岡県立大学薬学部生体機能分子分析学分野
  • URL: http://w3pharm.u-shizuoka-ken.ac.jp/~analchem/
• [Remarks] 所属研究室（静岡県立大学薬学部生体機能分子分析学分野）のHP
  • URL: http://w3pharm.u-shizuoka-ken.ac.jp/~analchem/