| Project/Area Number |
25461951
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
BANDO Yoshimi 徳島大学, 病院, 准教授 (00238239)
KURITA Nobuhiro 徳島大学, 病院, 特任教授 (30335814)
SATO Hirohiko 徳島大学, 病院, 特任助教 (80609694)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 腸管修復 / Sonic hedgehog / 幹細胞 / 腸管障害 / Shh pathway / Cancer stem cell |
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| Outline of Final Research Achievements |
This study focused on the relationship between the intestinal injury and Sonic hedgehog pathway. In intestinal injury models, Shh pathway was downregulated. HCT-116 sphere-derived cells showed significantly increased expression of stem cell markers, Shh downstream genes and epithelial-mesenchymal transition (EMT) markers compared to parental cells grown in conventional culture. The expression of stemness markers, Shh downstream genes and EMT markers were higher in cancer spheres than the parental cell line and downregulated by cyclopamine treatment in a dose-dependent manner. Shh pathway is related with cancer stem cell and may have an important role in intestinal injury.
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