The role of Sonic hedgehog in intestinal injury via stem cell
Project/Area Number |
25461951
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General surgery
|
Research Institution | The University of Tokushima |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
BANDO Yoshimi 徳島大学, 病院, 准教授 (00238239)
KURITA Nobuhiro 徳島大学, 病院, 特任教授 (30335814)
SATO Hirohiko 徳島大学, 病院, 特任助教 (80609694)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 腸管修復 / Sonic hedgehog / 幹細胞 / 腸管障害 / Shh pathway / Cancer stem cell |
Outline of Final Research Achievements |
This study focused on the relationship between the intestinal injury and Sonic hedgehog pathway. In intestinal injury models, Shh pathway was downregulated. HCT-116 sphere-derived cells showed significantly increased expression of stem cell markers, Shh downstream genes and epithelial-mesenchymal transition (EMT) markers compared to parental cells grown in conventional culture. The expression of stemness markers, Shh downstream genes and EMT markers were higher in cancer spheres than the parental cell line and downregulated by cyclopamine treatment in a dose-dependent manner. Shh pathway is related with cancer stem cell and may have an important role in intestinal injury.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Effect of Daikenchuto, a Traditional Japanese Herbal Medicine, after Total Gastrectomy for Gastric Cancer: A Multicenter, Randomized, Double-Blind, Placebo-Controlled, Phase II Trial.2015
Author(s)
Yoshikawa K, Shimada M, Wakabayashi G, Ishida K, Kaiho T, Kitagawa Y
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Journal Title
J Am Coll Surg
Volume: 221
Pages: 571-578
DOI
Related Report
Peer Reviewed
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[Journal Article] Characteristics of internal hernia after gastrectomy with Roux-en-Y reconstruction for gastric cancer.2014
Author(s)
Yoshikawa K, Shimada M, Kurita N, Sato H, Iwata T, Higashijima J, Chikakiyo M, Nishi M, Kashihara H, Takasu C, Matsumoto N, Eto S
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Journal Title
Surg Endosc
Volume: 28
Pages: 1774-1778
DOI
Related Report
Peer Reviewed
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[Journal Article] Hybrid technique for laparoscopic incisional ventral hernia repair combining laparoscopic primary closure and mesh repair.2014
Author(s)
Yoshikawa K, Shimada M, Kurita N, Sato H, Iwata T, Higashijima J, Chikakiyo M, Nishi M, Kashihara H, Takasu C, Matsumoto N, Eto S
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Journal Title
Hybrid technique for laparoscopic incisional ventral hernia repair combining laparoscopic primary closure and mesh repair.
Volume: 7
Pages: 282-285
DOI
Related Report
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[Presentation] 脂肪由来幹細胞の傷害肝へのホーミング効果―癌幹細胞転移メカニズムへの応用―2013
Author(s)
齋藤裕, 島田光生, 宇都宮徹, 池本哲也, 山田眞一郎, 森根裕二, 居村暁, 森大樹, 荒川悠佑, 金本真美, 岩橋衆一, 髙須千絵,
Organizer
第72回 日本癌学会学術総会
Place of Presentation
パシフィコ横浜(神奈川県)
Year and Date
20131003-20131005
Related Report
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[Presentation] ヒト脂肪由来幹細胞による肝傷害軽減効果に関する基礎的検討2013
Author(s)
齋藤裕, 島田光生, 宇都宮徹, 池本哲也, 山田眞一郎, 石川大地, 岩橋衆一, 金本真美, 荒川悠佑, 森大樹, 森根裕二, 居村暁
Organizer
第49回 日本移植学会総会
Place of Presentation
国立京都国際会館(京都府)
Year and Date
20130905-20130907
Related Report