| Project/Area Number |
25462236
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | Showa University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SHIODA SEIJI 星薬科大学, 先端生命科学研究所, 特任教授 (80102375)
Rakwal Randeep 筑波大学, 生命科学研究科, 教授 (70590850)
MIYAZAKI TAKURO 昭和大学, 医学部, 講師 (80398693)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 骨髄間葉系幹細胞 / 脳虚血 / マイクログリア / マイクロアレイ / 神経細胞死 / 移植細胞治療 / 炎症性サイトカイン |
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| Outline of Final Research Achievements |
Human bone marrow derived mesenchymal stem cells (hMSCs) attract the attention of tissue repair during several diseases. The purpose of the preset study is to determine the mechanism of hMSCs on tissue repair. In particular, we focused on the relationship between hMSCs and macrophages and microglia. hMSCs suppressed inducible nitric oxide synthase (iNOS) and NO production from BV-2 microglia after interferon-γ stimulation in the cell number dependent fashion. Moreover, by the transcriptome analysis, hMSCs cultured with ischemic hippocampal homogenate increased 98 transcriptomes including chemokines. Further analysis was determined a chemokine, CCL2 from hMSCs was regulated by inflammatory cytokines induced by ischemic stress.
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