Function analysis of TMEM16E using TMEM16E knockout mice

• Project/Area Number: 25463085
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: MIZUTA KUNIKO 広島大学, 医歯薬保健学研究院(歯), 助教 (40432679)
• Co-Investigator(Kenkyū-buntansha): TOBIUME KEI 広島大学, 大学院医歯薬保健学研究院(歯), 准教授 (40350037)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: TMEM16E / ノックアウトマウス / 筋ジストロフィー / GDD / 顎骨骨幹異形成症

Outline of Final Research Achievements

In this study, TMEM16E knockout mice showed no phenotypic signs. The result suggested that the function of TMEM16E was compensated by other muscular dystrophy related molecules. During in vitro human myogenic cell differentiation, TMEM16E protein expression was up-regulated in mitotic myoblasts as well as myotubes. This finding suggests that TMEM16E protein expression is regulated in a cell cycle dependent manner.

Research Products

• [Journal Article] CD44(high) /ALDH1(high) head and neck squamous cell carcinoma cells exhibit mesenchymal characteristics and GSK3β-dependent cancer stem cell properties. (2016)
  • Author(s): Seino S, Shigeishi H, Hashikata M, Higashikawa K, Tobiume K, Uetsuki R, Ishida Y, Sasaki K, Naruse T, Rahman MZ, Ono S, Simasue H, Ohta K, Sugiyama M, Takechi M.
  • Journal Title: J Oral Pathol Med Volume: 45 Issue: 3 Pages: 180-188
  • DOI: 10.1111/jop.12348
  • Peer Reviewed / Open Access
• [Journal Article] Preoperative oral health care reduces postoperative inflammation and complications in oral cancer patients. (2015)
  • Author(s): Shigeishi H, Ohta K, Fujimoto S, Nakagawa T, Mizuta K, et al.
  • Journal Title: Exp Ther Med Volume: in press
  • Peer Reviewed
• [Journal Article] Expression and function of RIG-I in oral keratinocytes and fibroblasts (2014)
  • Author(s): Ohta K, Fukui A
  • Journal Title: Cell Phsiol Biochem Volume: 34 Issue: 5 Pages: 1556-1565
  • DOI: 10.1159/000366359
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Toll-like receptor (TLR) expression and TLR‑mediated interleukin-8 production by human submandibular gland epithelial cells. (2014)
  • Author(s): Ohta K, Ishida Y, Fukui A, Mizuta K, Nishi H, Takechi M, Kamata N.
  • Journal Title: Molecular medicine reports. Volume: 10（5） Issue: 5 Pages: 2377-2382
  • DOI: 10.3892/mmr.2014.2507
  • Peer Reviewed / Open Access
• [Journal Article] TMEM16E (GDD1) exhibits protein instability and distinct characteristics in chloride channel/pore forming ability. (2014)
  • Author(s): Tran TT, Tobiume K, Hirono C, Fujimoto S, Mizuta K, Kubozono K, Inoue H, Itakura M, Sugita M, Kamata N.
  • Journal Title: J Cell Physiol. Volume: 229(2) Issue: 2 Pages: 181-190
  • DOI: 10.1002/jcp.24431
  • Peer Reviewed
• [Presentation] 顎骨骨幹異形成症および肢帯型筋ジストロフィー原因遺伝子TMEM16E/GDD1の機能解析 (2014)
  • Author(s): 久保薗和美，水田邦子，藤本伸一，武知正晃
  • Organizer: 第59回(公社)日本口腔外科学会総会・学術大会
  • Place of Presentation: 幕張メッセ 国際会議場
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] 術前PET/CTによる口腔扁平上皮癌の予後予測の可能性に関する臨床統計的検討 (2014)
  • Author(s): 水田邦子，太田耕司，藤本伸一，植月亮，小川郁子，武知正晃
  • Organizer: 第59回(公社)日本口腔外科学会総会・学術大会
  • Place of Presentation: 幕張メッセ 国際会議場
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] Characterization of TMEM16E/GDD1 that causes Gnatho-Diaphyseal Dysplasia and Limb-girdle muscular dystrophy through distinct gene mutations. (2014)
  • Author(s): Kubozono K, Mizuta K, Fujimoto S, Takechi M.
  • Organizer: 96th AAOMS Annual Meeting, Scientific Session and Exhibitionin conjunction with the Japanese Society and Korean Association of Oral and Maxillofacial Surgeons
  • Place of Presentation: Hawaii Convention Center (Honolulu, USA)
  • Year and Date: 2014-09-11
• [Presentation] Study of prognostic prediction using pre-treatment PET/CT in oral cancer patients. (2014)
  • Author(s): Mizuta K, Ohta K, Fujimoto S, Uetsuki R, Takechi M.
  • Organizer: 96th AAOMS Annual Meeting, Scientific Session and Exhibitionin conjunction with the Japanese Society and Korean Association of Oral and Maxillofacial Surgeons
  • Place of Presentation: Convention Center (Honolulu, USA)
  • Year and Date: 2014-09-11
• [Presentation] Functional analysis of gene TMEM16E/GDD1 that cause Gnatho-Diaphyseal Dysplasia and Limb-girdle muscular dystrophy. (2014)
  • Author(s): Kubozono K, Mizuta K, Fujimoto S, Takechi M .
  • Organizer: 第47回広島大学歯学会総会
  • Place of Presentation: 広島大学歯学部
  • Year and Date: 2014-06-21
• [Presentation] Prognostic utility of standardized uptake value using pre-treatment PET/CT in oral cancer patients. (2014)
  • Author(s): Mizuta K, Ohta K, Ono S, Nakagawa T, Fujimoto S, Okumura T, Uetsuki R, Takechi M.
  • Organizer: 第47回広島大学歯学会総会
  • Place of Presentation: 広島大学歯学部
  • Year and Date: 2014-06-21
• [Presentation] 顎骨骨幹異形成症原因遺伝子GDD1の機能解析 (2013)
  • Author(s): 久保薗和美
  • Organizer: 日本口腔外科学会総会・学術大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡