Development of highly efficient labelling reagents for protein
| Project/Area Number |
25670058
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Drug development chemistry
|
|---|
| Research Institution | The University of Tokushima |
|---|
Principal Investigator |
OTAKA Akira 徳島大学, ヘルスバイオサイエンス研究部, 教授 (20201973)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | タンパク質 / ラベル化 / 標的タンパク質 / N-Sアシル転移 / タンパク質ラベル化 / チオエステル / ケミカルバイオロジー |
|---|
| Outline of Final Research Achievements |
In cell labeling of proteins is an essential technology for elucidating the function of proteins in living cells. Among various strategies, an affinity labeling with the use of protein ligands and electrophilic moieties has been widely used. Achievement of an affinity labeling requires the tuning of reactivity of an electrophilic moiety to prevent non-specific labeling. Recently, we reported the N-sulfanylethylanilide (SEAlide) as a tunable electrophilic moiety. Although the SEAlide, an anilide compound, almost remains intact, addition of phosphate salts as acid-based catalyst induces conversion of the stable anilide linkage to the the electrophilic thioester via N-S acyl transfer. On the basis of this interesting feature of the SEAlide, we developed highly efficient labelling reagnets for proteins with practical application of labelling of model proteins using SEAlide based reagents.
|
Report
(3 results)
Research Products
(32 results)
-
-
-
[Journal Article] Development of a fluoride-responsive amide bond cleavage device that is potentially applicable to a traceable linker2014
Author(s)
J. Yamamoto, N. Maeda, C. Komiya, T. Tanaka, M. Denda, K. Ebisuno, W. Nomura, H. Tamamura, Y. Sato, A. Yamauchi, A. Shigenaga, and A. Otaka
-
Journal Title
Tetrahedron
Volume: 70
Issue: 34
Pages: 5122-5127
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
[Journal Article] Development of a traceable linker containing a thiol-responsive amino acid for the enrichment and selective labelling of target proteins2014
Author(s)
J. Yamamoto, M. Denda, N. Maeda, M. Kita, C. Komiya, T. Tanaka, W. Nomura, H. Tamamura, Y. Sato, A. Yamauchi, A. Shigenaga, and A. Otaka
-
Journal Title
Org. Biomol. Chem.
Volume: 12
Issue: 23
Pages: 3821-3826
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
[Presentation] M6P修飾型GM2APの合成検討2015
Author(s)
成瀬 公人, 佐藤 浩平, 坂本 健, 猪熊 翼, 重永 章, 大髙 章
Organizer
日本薬学会第135年会
Place of Presentation
神戸学院大学（兵庫県神戸市）
Year and Date
2015-03-25 – 2015-03-28
Related Report
-
-
-
-
-
[Presentation] ペプチドチオエステル調製のためのクマリン型補助基の開発2014
Author(s)
江藤 三弘, 傳田 将也, 佐藤 浩平, 坂本 健, 猪熊 翼, 重永 章, 大髙 章
Organizer
第53回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
Place of Presentation
広島国際会議場（広島県広島市）
Year and Date
2014-11-08 – 2014-11-09
Related Report
-
[Presentation] 新規タンパク質チオエステル調製法の開発2014
Author(s)
津田 雄介, 重永 章, 傳田 将也, 佐藤 浩平, 北風 圭介, 中村 太寛, 猪熊 翼, 伊藤 孝司, 大髙 章
Organizer
第53回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
Place of Presentation
広島国際会議場（広島県広島市）
Year and Date
2014-11-08 – 2014-11-09
Related Report
-
-
-
-
-
-
-
-
-
[Presentation] 過酸化水素応答型ペプチド結合切断デバイスの開発研究2014
Author(s)
北 未来, 山本 純, 戎野 紘司, 小宮 千明, 宮本 理人, 土屋 浩一郎, 重永 章, 大髙 章
Organizer
日本ケミカルバイオロジー学会第9回年会
Place of Presentation
大阪大学（大阪府豊中市）
Year and Date
2014-06-11 – 2014-06-13
Related Report
-
-
-
-
-
-
