Promoting differentiation of osteoblasts from induced pluripotent stem (iPS) cells using scaffolds recreating the bone tissue microenvironment.

• Project/Area Number: 25670859
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: MIYAMOTO Youji 徳島大学, ヘルスバイオサイエンス研究部, 教授 (20200214)
• Co-Investigator(Kenkyū-buntansha): NAGAI Hirokazu 徳島大学, 大学院ヘルスバイオサイエンス研究部, 准教授 (50282190) ; TAMATANI Tetsuya 徳島大学, 病院, 講師 (30274236) ; UCHIDA Daisuke 獨協医科大学, 医学部, 准教授 (20335798)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 再生医学 / iPS細胞 / 骨再生 / スキャフォールド / 骨芽細胞

Outline of Final Research Achievements

In this study, we try to promote osteoblastic differentiation of human induced pluripotent stem (iPS) cells using scaffolds recreating the bone tissue microenvironment.
As a result, it was revealed that 1) carbonate apatite (CAp) foam was fabricated based on compositional conversion reaction using α-tricalcium phosphate foam as a precursor, 2) low-crystalline CAp foam with 93%～96% porosity and fully interconnected porous structure was fabricated, 3) the differentiation of human iPS cells to mesenchymal stem cell-like cells was induced, 4) CAp was resorbed by osteoclast in vivo, 5) mesenchymal stem cells induced from iPS cells cultured on carbonate apatite differentiated into osteoblastic cells, 6) CAp granules combined with BMP-2 stimulated the ectopic new bone formation by promoting osteoblastic differentiation of mesenchymal cells in the surrounding tissue.

Research Products

• [Journal Article] Effects of low crystalline carbonate apatite on proliferation and osteoblastic differentiation of human bone marrow cells (2015)
  • Author(s): Hirokazu Nagai, Masako Kobayashi-Fujioka, Kenji Fujisawa, Go Ohe, Natsumi Takamaru, Kanae Hara, Daisuke Uchida, Tetsuya Tamatani, Kunio Ishikawa, Youji Miyamoto
  • Journal Title: J Mater Sci: Mater Med Volume: 26 Issue: 2 Pages: 99-99
  • DOI: 10.1007/s10856-015-5431-5
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 炭酸アパタイト被覆炭酸カルシウムを用いたハイブリッド型骨補填材料による骨再生の試み (2014)
  • Author(s): 小林真左子、永井宏和、藤澤健司、都留寛治、石川邦夫、山中克之、熊谷知弘、宮本洋二
  • Organizer: 第36回日本バイオマテリアル学会大会
  • Place of Presentation: タワーホール船堀(東京都江戸川区）
  • Year and Date: 2014-11-17 – 2014-11-18
• [Presentation] 炭酸アパタイト被膜炭酸カルシウムを用いた新規骨置換材料による骨再生の試み (2014)
  • Author(s): 小林真左子、永井宏和、原 香苗、鎌田久美子、工藤隆治、筋澤健司、宮本洋二
  • Organizer: 第59回（公社）日本口腔外科学会総会・学術大会
  • Place of Presentation: 幕張メッセ（千葉県千葉市）
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] Nobel Bone Regeneration System Using Carbonate Apatite-coated Carbonate Calcium in Vivo (2014)
  • Author(s): Masako Kobayashi, Hirokazu Nagai, Kanae Hara, Kenji Fujisawa, Daisuke Uchida, Tetsuya Tamatani, Youji Miyamoto
  • Organizer: American association of Oral Maxillofacial Surgeons(aaoms) 96th Annual Meeting, scientific Sessions & Exhibition
  • Place of Presentation: Hawaii Convention center ホノルル（USA)
  • Year and Date: 2014-09-08 – 2014-09-13
• [Presentation] Application of Low Crystalline Carbonate Apatite Combined With BMP-2 to Bone Reconstruction (2014)
  • Author(s): Hirokazu Nagai , Kanae Hara , Masako Kobayashi , Go Ohe , Tetsuya Tamatani, Kenji Fujisawa, Youji Miyamoto
  • Organizer: American association of Oral Maxillofacial Surgeons(aaoms) 96th Annual Meeting, scientific Sessions & Exhibition
  • Place of Presentation: Hawaii Convention center ホノルル（USA)
  • Year and Date: 2014-09-08 – 2014-09-13
• [Presentation] 低結晶性炭酸アパタイトの顎骨再建への応用に関する基礎的研究 第6報 BMP-2との併用効果 (2013)
  • Author(s): 永井宏和、原香苗、小林真左子、藤澤健司、都留寛治、山本克史、石川邦夫、宮本洋二
  • Organizer: 第35回日本バイオマテリアル学会大会
  • Place of Presentation: タワーホール船堀（東京都）