|Budget Amount *help
¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
|Outline of Final Research Achievements
Upon administration of 0.1% BBN for 20 weeks after inducing two types of TP53 point mutations (R172H, R270H) in the urothelial cells expressing Krt5 or Upk2 by using the lox - stop - lox system, bladder cancer tissues originating from those mutant cells were obtained. Mouse bladder cancer tissues of each lineage were compared by pathological evaluation and expression microarray, and as a result, when TP 53 point mutation was induced in Krt5 expressing cells, muscle invasive bladder cancer with squamous differentiation, similar to human bladder cancer belonging to TCGA cluster 3, was generated most efficiently.