Alternative polyadenylation in lung cancer
Project/Area Number |
25830098
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Tumor diagnostics
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Research Institution | The University of Tokyo |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | 肺がん / マイクロRNA / 選択的ポリアデニル化 / 転写後制御 / バイオマーカー / 非小細胞肺癌 |
Outline of Final Research Achievements |
Alternative polyadenylation (APA), which induces shortening of the 3'UTR, is emerging as an important phenomenon in gene regulation. We identified 10 genes showing significant 3'UTR shortening in lung cancer, using the package of the Bioconductor for probe-level analyses of expression microarrays. We established and evaluated the APA score by quantitative RT-PCR in clinical specimens of non-small cell lung cancer, amd found that high APA score was an independet prognostic factor. In addition, both lower expression of PABPN1 and higher expression of the proliferation markers were correlated with high APA scores and a poor prognosis. These results suggest that the loss of PABPN1, a suppressor of APA, might promote tumor aggressiveness by releasing the cancer cells from microRNA-mediated gene regulation.
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Report
(3 results)
Research Products
(6 results)
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[Journal Article] Oncogenic TPM3-ALK activation requires dimerization through the coiled-coil structure of TPM32015
Author(s)
Amano Y, Ishikawa R, Sakatani T, Ichinose J, Sunohara M, Watanabe K, Kage H, Nakajima J, Nagase T, Ohishi N, Takai D
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Journal Title
Biochem Biophys Res Commun
Volume: 13
Issue: 3
Pages: 457-60
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Histone methylation-mediated silencing of mir-139 enhances an aggressive phenotype of non-small cell lung cancer2014
Author(s)
Kousuke Watanabe, Mitsuhiro Sunohara, Yosuke Amano, Rie Ishikawa, Junji Ichinose, Jun Nakajima, Masashi Fukayama, Yutaka Yatomi, Takahide Nagase, Nobuya Ohishi, and Daiya Takai
Organizer
AACR-IASLC Joint Conference on Molecular Origins of Lung Cancer
Place of Presentation
San Diego, USA
Related Report