Observation of structural dynamics of microtubules by optical second-harmonic generation
Project/Area Number |
25871144
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Biophysics
Biological physics/Chemical physics/Soft matter physics
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Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
KANESHIRO Junichi 独立行政法人理化学研究所, 生命システム研究センター, 訪問研究員 (90628471)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
|
Keywords | 微小管 / 光第二高調波発生 / 構造生物学 / 光第二高調波 |
Outline of Final Research Achievements |
Label-free single microtubules are successfully observed both in vitro and in cells with the laser-scanning optical second harmonic generation (SHG) microscopy technique. Sufficient sensitivity to detect the SHG signal from a single microtubule with high signal-to-noise ratio is achieved by simple reduction of possible noise sources such as external SHG radiation from some optical elements, environmental light, cosmic ray. Polarization dependence of SHG intensity is measured for each of GMPCPP (analogue of GTP) bound and GDP-taxol bound microtubules to analyze their SHG tensors which are related to electronic polarization states. The SHG tensors of GMPCPP- and GDP-taxol- microtubules are statistically different, and it is indicated that the angle of a dipole moment in a tubulin heterodimer which mainly consists of alpha-helices changes through hydrolysis of GTP. The result is consistent with the already reported cryo-electron microscopy study.
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Report
(3 results)
Research Products
(3 results)