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Identification of non-coding RNA associated with the proliferative and chondrogenic abilities of human bone marrow mesenchymal stem cells

Research Project

Project/Area Number 25893277
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionMukogawa Women's University

Principal Investigator

MERA Hisashi  武庫川女子大学, スポーツ健康科学部, 研究員 (70650381)

Project Period (FY) 2013-08-30 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords関節軟骨修復・再生 / 骨髄細胞 / 軟骨分化度マーカー / miR708-5p / 関節軟骨修復 / 軟骨分化能 / non-coding RNA
Outline of Final Research Achievements

In this study, we analyzed the bone marrow mesenchymal stem cells (BMSCs) isolated from 30 donors. We could repeat the quantitative outcomes that were previously reported for the effects of bFGF on the chondrogenic ability of the BMSCs during the expansion phase. Moreover, we could obtain independent quantitative data for each donor. This finding indicates that the sample size used here is sufficient for subsequent analysis to validate the results.
The RNA profile of each donor, generated before BMSC differentiation, was analyzed comprehensively using an RNA microarray system (EXIQON Inc. miRCURY LNA miRNA array). We selected some candidate micro RNAs as markers of chondrogenic ability of BMSCs. These markers were verified by quantitative PCR, and miR708-5p was found to suppress chondrogenic differentiation. However, the variation in miR708-5p expression between donors was large, suggesting that other factors could also be involved in regulating the chondrogenic ability of BMSCs.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report

Research Products

(9 results)

All 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (7 results) (of which Invited: 3 results)

  • [Journal Article] Potential for Tumorigenesis and Repair Osteochondral Defects by iPS cell Transplantation in Rat.2014

    • Author(s)
      Mera H., Takigami J., Tamamura Y., Itokazu M., Yamazoe M., Hashimoto Y., Endo N., Wakitani S.
    • Journal Title

      American Journal of Tissue Engineering and Stem cell.

      Volume: 1(1) Pages: 39-50

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cartilage Repair With Autologous Bone Marrow Mesenchymal Stem Cell Transplantation: Review of Preclinical and Clinical Studies.2014

    • Author(s)
      Yamasaki S., Mera H., Itokazu M., Hashimoto Y., Wakitani S.
    • Journal Title

      Cartilage

      Volume: 5(4) Pages: 196-202

    • DOI

      10.1177/1947603514534681

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] 骨髄間葉系細胞を用いた関節軟骨再生治療の研究開発2015

    • Author(s)
      目良恒、石橋宰、高木睦、脇谷滋之
    • Organizer
      第7回 日本関節鏡・膝・スポーツ整形外科学会(JOSKAS)
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2015-06-18 – 2015-06-20
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] The population of human bone marrow mesenchymal stem cells supplemented with basic fibroblast growth factor for chondrogenic differentiation.2015

    • Author(s)
      Hisashi Mera, Yoshihiro Tamamura, Maki Itokazu, Miki Asada, Shigeyuki Wakitani
    • Organizer
      Osteoarthritis Research Society International
    • Place of Presentation
      Washington State Convention Center, Seattle, WA, (USA)
    • Year and Date
      2015-04-30 – 2015-05-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] 変形性関節症と再生医療2014

    • Author(s)
      目良恒、脇谷滋之
    • Organizer
      第58回 日本リウマチ学会総会・学術集会
    • Place of Presentation
      グランドプリンスホテル新高輪(東京都・港区)
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ヒト骨髄細胞由来scaffold-free再生軟骨様細胞シ-ト作製の工夫2014

    • Author(s)
      糸数万紀、目良恒、佐藤康史、中村博亮、脇谷滋之
    • Organizer
      第27回 日本軟骨代謝学会
    • Place of Presentation
      京都府医師会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒト骨髄細胞由来scaffold-free再生軟骨様細胞シ-ト作製の工夫2014

    • Author(s)
      目良恒、糸数万紀、高木睦、脇谷滋之
    • Organizer
      第217回 新潟整形外科研究会
    • Place of Presentation
      新潟大学医学部有壬記念館
    • Related Report
      2013 Annual Research Report
  • [Presentation] 変形性関節症と再生医療2014

    • Author(s)
      目良恒、脇谷滋之
    • Organizer
      第58回 日本リウマチ学会
    • Place of Presentation
      グランドプリンスホテル新高輪
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] THE ENHANCEMENT OF HUMAN BONE MARROW DERIVED MESENCHYMAL STEM CELLS (HBMSCS) FOR SCAFFOLD-FREE CARTILAGE-LIKE CELL-SHEETS2014

    • Author(s)
      Maki Itokazu, Hisashi Mera, Yasushi Sato, Hiroaki Nakamura, Mutsumi Takagi, Shigeyuki Wakitani
    • Organizer
      2014 World congress on Osteoarturitis (OARSI)
    • Place of Presentation
      The center of New Industries and Technologies, Paris, France
    • Related Report
      2013 Annual Research Report

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Published: 2013-09-12   Modified: 2019-07-29  

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