Gene regulatory network underlying epiblast cell determination and their derivation into various somatic cell lineages
| Project/Area Number |
26251024
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto Sangyo University |
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Principal Investigator |
KONDOH Hisato 京都産業大学, 総合生命科学部, 教授 (70127083)
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| Co-Investigator(Renkei-kenkyūsha) |
ISHII Yasuo 東京女子医科大学, 医学部, 講師 (20582430)
TAKEMOTO Tatsuya 徳島大学, 先端酵素学研究所, 教授 (30443899)
ARUGA Jun 長崎大学, 医歯薬学総合研究科, 教授 (10232076)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥40,430,000 (Direct Cost: ¥31,100,000、Indirect Cost: ¥9,330,000)
Fiscal Year 2016: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2015: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2014: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
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| Keywords | 遺伝子発現調節 / 転写因子 / エピブラスト / 幹細胞 / 体細胞系列 / 胚発生 / ノード / エピブラスト幹細胞 / マウス胚操作 |
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| Outline of Final Research Achievements |
Diverse somatic cells develop from pluripotent epiblast cells that are established after embryo implantation. However, the gene regulatory network underlying epiblast cell state determination and that involved in somatic cell derivation remains unclear. We investigated the regulatory function of major transcription factors (TFs) in the epiblast, and showed a drastic change in the gene regulatory network in the epiblast compared with the preimplantation pluripotent state. To characterize the process of somatic cell derivation, we established stem cell lines that represent early somatic lineages, e.g., neural progenitor cell lines with anteroposterior regional specificities, and characterized these intermediate somatic cell states. In addition, we investigated the regulatory function of the TF SOX2 in determining the regional specificity of endodermal tissues.
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Report
(4 results)
Research Products
(54 results)
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[Journal Article] Sip1 regulates the generation of the inner nuclear layer retinal cell lineages in mammals.2016
Author(s)
Menuchin-Lasowski Y., Oren-Giladi P., Xie Q., Ezra-Elia R., Ofri R., Peled-Hajaj S., Farhy C., Higashi Y., Van de Putte T., Kondoh H., Huylebroeck D., Cvekl A., Ashery-Padan R.
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Journal Title
Development. 143:2829-2841 (2016)
Volume: 143
Issue: 15
Pages: 829-2841
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Transcriptional repressor ZEB2 promotes terminal differentiation of CD8+ effector and memory T cell populations during infection.2015
Author(s)
Omilusik KD, Best JA, Yu B, Goossens S, Weidemann A, Nguyen JV, Seuntjens E, Stryjewska A, Zweier C, Roychoudhuri R, Gattinoni L, Bird LM, Higashi Y, Kondoh H, Huylebroeck D, Haigh J, Goldrath AW.
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Journal Title
J Exp Med.
Volume: 12
Issue: 12
Pages: 2027-2039
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Two classes of Sox2-partner factor interaction involved in developmental gene regulation, as indicated by genome-wide ChIP-seq analaysis.2015
Author(s)
Kondoh H, Matsuda, K, Mikami T, Andrabi M, Yamaguchi K, Shigenobu S.
Organizer
48th Annual meetin of the Japanese Society of Developmental Biologists.
Place of Presentation
Tsukuba Convention Center, Tsukuba, Ibaragi, Japan
Year and Date
2015-06-02
Related Report
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