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Development of core technologies for improving existence of bacteria released to the environment to attain an efficient bio-augmentation

Research Project

Project/Area Number 26289184
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Civil and environmental engineering
Research InstitutionTohoku Gakuin University

Principal Investigator

Nakamura Kanji  東北学院大学, 工学部, 教授 (90382655)

Co-Investigator(Kenkyū-buntansha) 宮内 啓介  東北学院大学, 工学部, 教授 (20324014)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywordsバイオオーグメンテーション / 環境浄化細菌 / 捕食 / トリクロロエチレン / ビオラセイン / 原生動物 / 鞭毛虫 / バイオオーグメンテーション / 蛍光タンパク質 / トランスポゾン / トリプトファン / 被食 / べん毛虫
Outline of Final Research Achievements

Development of core technologies for efficient bio-augmentation treatment was examined. As a main technology, introduction of violacein-producing genes, vioABCDE, to two types of Cupriavidus sp. bacteria, that had strong TCE degradability, were examined to confer antiprotozoal property. The genes of vioABCDE were integrated into chromosome DNA of Cupriavidus sp. bacteria by using a Tn5 transposon vector. Colonies of transconjugants showed violet color due to violacein production. Transconjugants that showed deeper violet color were selected among transconjugants. Two selected transconjugants that derived from each parent strain were shown to avoid predation by a flagellate, Spumella. These transconjugants also maintained their original TCE degradability derived from their parent strain.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (5 results)

All 2017 2016 2015 2014

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 4 results,  Open Access: 1 results) Presentation (1 results)

  • [Journal Article] INTRODUCTION OF VIOLACEIN PRODUCING GENES INTO TRICHLOROETHENE-DEGRADING BACTERIA TO AVOID PROTOZOAN PREDATION2017

    • Author(s)
      中村 寛治,加藤 俊明,石川 廣大,柴崎 純一
    • Journal Title

      Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research)

      Volume: 73 Issue: 2 Pages: 39-46

    • DOI

      10.2208/jscejer.73.39

    • NAID

      130006904165

    • ISSN
      2185-6648
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] ANALYSIS OF TRICHLOROETHENE DEGRADATION BY PHENOL HYDROXYLASE USING FLUORESCENT PROTEIN AS A REPORTER2016

    • Author(s)
      中村 寛治,渡辺 健幸
    • Journal Title

      Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research)

      Volume: 72 Issue: 7 Pages: III_275-III_283

    • DOI

      10.2208/jscejer.72.III_275

    • NAID

      130005530494

    • ISSN
      2185-6648
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 原生動物の捕食挙動解析を目的とした被食細菌への蛍光タンパク質遺伝子の導入2015

    • Author(s)
      中村 寛治,渡辺 健幸
    • Journal Title

      環境工学研究論文集G(環境)

      Volume: 71 Pages: 143-151

    • NAID

      130005114800

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 原生動物の捕食回避を目的としたビオラセイン合成遺伝子群の取得および発現2014

    • Author(s)
      中村 寛治、渡邉 暁、成田 賢人
    • Journal Title

      環境工学研究論文集G(環境)

      Volume: 70 Pages: 39-46

    • NAID

      130004962652

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Cupriavidus nector KT1によるビオラセイン合成遺伝子の発現および原生動物による捕食の回避2016

    • Author(s)
      加藤 俊明,中村 寛治
    • Organizer
      土木学会,環境工学研究フォーラム
    • Place of Presentation
      北九州国際会議場(福岡県・北九州市)
    • Year and Date
      2016-12-06
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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