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Identification of novel pathways consist of DGK isozymes using new analyzing methods and regulation of their functions

Research Project

Project/Area Number 26291017
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Functional biochemistry
Research InstitutionChiba University

Principal Investigator

Sakane Fumio  千葉大学, 理学(系)研究科(研究院), 教授 (10183815)

Co-Investigator(Renkei-kenkyūsha) SAKAI HIROMICHI  島根大学, 総合科学研究支援センター, 助教 (00375255)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords細胞情報伝達機構 / 脂質 / ジアシルグリセロール / リン酸化 / 活性制御 / ジアシルグリセロールキナーゼ / がん / 双極性障害 / 強迫性障害 / 糖尿病 / シヌクレイン / モノアシルグリセロール / ミリスチン酸 / シグナル伝達 / 神経細胞分化 / ホスファチジルイノシトール / ホスファチジルコリン / ホスホリパーゼC / 歯状回 / アポトーシス
Outline of Final Research Achievements

Diacylglycerol kinase (DGK) contains ten isozymes. We have recently found that DGK isozymes play pivotal roles in a wide variety of signal transduction pathways and are involved in pathogenesis of refractory diseases. Thus, we planned to find (1) novel pathways consist of DGK isozymes and to reveal their patho-physiological functions. We obtained interesting results concerning new metabolic pathways that are composed of DGKα, δ, η and ζ, and their patho-physiological functions (e.g., cancer, immunity, bipolar disorder, obsessive-compulsive disorder, and Parkinson’s disease).

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(72 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (20 results) (of which Peer Reviewed: 19 results,  Open Access: 18 results,  Acknowledgement Compliant: 20 results) Presentation (44 results) (of which Int'l Joint Research: 7 results,  Invited: 3 results) Book (1 results) Remarks (3 results) Patent(Industrial Property Rights) (2 results)

  • [Int'l Joint Research] Cambridge University(英国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] IGBMC(フランス)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Dioleoyl-phosphatidic acid selectively binds to α-synuclein and strongly induces its aggregation2017

    • Author(s)
      Satoru Mizuno, Hirotaka Sasai, Aiko Kume, Mamoru Satoh, Sayaka Kado, Fumio Sakane
    • Journal Title

      FEBS Lett.

      Volume: 591 Pages: 784-791

    • DOI

      10.1002/1873-3468.12592

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Development of a liquid chromatography-mass spectrometry based enzyme activity assay for phosphatidylcholine-specific phospholipase C.2017

    • Author(s)
      Murakami, C., Mizuno, S., Kado, S. and Sakane, F.
    • Journal Title

      Anal. Biochem.

      Volume: 526 Pages: 43-49

    • DOI

      10.1016/j.ab.2017.03.010

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] “Deficiency of diacylglycerol kinase h induces lithium-sensitive mania-like behavior.2016

    • Author(s)
      Isozaki T., Komenoi S., Lu Q., Usuki T., Tomokata S., Usuki T., Tomokata S., Matsutomo D., Sakai H., Bando K., Kiyonari H., Sakane F.
    • Journal Title

      J. Neurochem.

      Volume: 138 Pages: 448-456

    • DOI

      10.1111/jnc.13661

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Myristic acid enhances diacylglycerol kinase δ-dependent glucose uptake in myotubes.2016

    • Author(s)
      Wada, Y., Sakiyama, S., Sakai, H. and Sakane, F.
    • Journal Title

      Lipids

      Volume: 51 Pages: 897-903

    • DOI

      10.1007/s11745-016-4162-9

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Distinct 1- and 2-monoacylglycerol kinase activities of diacylglycerol kinase isozymes.2016

    • Author(s)
      Sato, Y., Murakami, C., Yamaki, A., Mizuno, S., Sakai, H. and Sakane, F.
    • Journal Title

      Biochim. Biophys. Acta. - Proteins Proteomics

      Volume: 1864 Pages: 1170-1176

    • DOI

      10.1016/j.bbapap.2016.06.012

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Behavioral and pharmacological phenotypes of brain-specific diacylglycerol kinase δ-knockout mice.2016

    • Author(s)
      Usuki, T., Takato, T., Lu Q., Sakai, H., Bando, K., Kiyonari, H. and Sakane, F.
    • Journal Title

      Brain Res.

      Volume: 1648 Pages: 193-201

    • DOI

      10.1016/j.brainres.2016.07.017

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Activation of conventional and novel protein kinase C isozymes by different diacylglycerol molecular species.2016

    • Author(s)
      Kamiya, Y., Mizuno, S., Komenoi, S., Sakai, H., Sakane, F.
    • Journal Title

      Biochem. Biophys. Rep.

      Volume: 7 Pages: 361-366

    • DOI

      10.1016/j.bbrep.2016.07.017

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cloning and characterization of novel testis-specific diacylglycerol kinase η splice variant 3 and 4.2016

    • Author(s)
      Murakami, E., Shionoya, T. Komenoi, S., Suzuki, Y., Sakane, F.
    • Journal Title

      PLOS ONE

      Volume: 11 Pages: 1-14

    • DOI

      10.1371/journal.pone.0162997

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Diacylglycerol kinase ζ generates dipalmitoyl-phosphatidic acid species during neuroblastoma cell differentiation2016

    • Author(s)
      Satoru Mizuno, Sayaka Kado, Kaoru Goto, Daisuke Takahashi, Fumio Sakane
    • Journal Title

      Biochem. Biophys. Rep.

      Volume: 8 Pages: 352-359

    • DOI

      10.1016/j.bbrep.2016.10.004

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Diacylglycerol Kinases as Emerging Potential Drug Targers for a Variety of Diseases: An Update.2016

    • Author(s)
      Sakane F., Mizuno S., Komenoi S.
    • Journal Title

      Front. Cell Dev. Biol.

      Volume: 4 Pages: 1-8

    • DOI

      10.3389/fcell.2016.00082

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A novel diacylglycerol kinase α-selective inhibitor, CU-3, induces cancer cell apoptosis and enhances immune response2016

    • Author(s)
      1)Lui K, Kunii N, Sakuma M, Yamaki A, Mizuno S, Sato M, Sakai H, Kado S, Kumagai K, Kojima H, Okabe T, Nagano T, Shirai Y. and Sakane F
    • Journal Title

      J Lipid Res.

      Volume: 57 Pages: 368-379

    • DOI

      10.1194/jlr.m062794

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The pleckstrin homology domain of diacylglycerol kinase η strongly and selectively binds to phosphatidylinositol 4,5-bisphosphate.2016

    • Author(s)
      Kume, A., Kawase, K., Komenoi, S., Usuki, T., Takeshita, E., Sakai, H. and Sakane, F.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 291 Pages: 8150-8161

    • DOI

      10.1074/jbc.m115.648717

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Deficiency of diacylglycerol kinase η induces lithium-sensitive mania-like behaviors.2016

    • Author(s)
      Isozaki, T., Komenoi, S., Lu Q., Usuki, T., Tomokata, S., T., Matsutomo, D., Sakai, H. Bando, K., Kiyonari, H. and Sakane, F.
    • Journal Title

      J. Neurochem.

      Volume: 137

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Diacylglycerol kinases η1 is a high affinity isozyme for diacylglycerol2015

    • Author(s)
      Komenoi, S., Takemura, F., Sakai, H. and Sakane, F.
    • Journal Title

      FEBS Lett.

      Volume: in press Pages: 1272-1277

    • DOI

      10.1016/j.febslet.2015.03.032

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Expression and localization of type II diacylglycerol kinase isozymes δ and η in developing mouse brain2015

    • Author(s)
      Usuki, T., Sakai, H., Shionoya, T., Sato, N. and Sakane, F.
    • Journal Title

      J. Histochem. Cytochem.

      Volume: 63 Pages: 57-68

    • DOI

      10.1369/0022155414559130

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Distinct expression and localization of type II diacylglycerol kinase isozymes δ, η and κ in mouse reproductive organs2015

    • Author(s)
      Shionoya, T., Usuki, T., Komenoi, S., Isozaki, T., Sakai, H. and Sakane, F.
    • Journal Title

      BMC Dev. Biol.

      Volume: 15

    • DOI

      10.1186/s12861-015-0055-z

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Both the C1 domain and a basic amino acid cluster at C-terminus are important for neurite and branch induction ability of DGKb2014

    • Author(s)
      Takuya Kano, Takeshi Kouzuki, Satoru Mizuno, Shuji Ueda, Minoru Yamanoue, Fumio Sakane, Naoaki Saito and Yasuhito Shirai
    • Journal Title

      Takuya Kano, Takeshi Kouzuki, Satoru Mizuno, Shuji Ueda, Minoru Yamanoue, Fumio Sakane, Naoaki Saito and Yasuhito Shirai

      Volume: 447 Pages: 89-94

    • DOI

      10.1016/j.bbrc.2014.03.113

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] EF-hand motifs of diacylglycerol kinase α interact intra-molecularly with its C1 domains2014

    • Author(s)
      Yamamoto, T., Sakai, H. and Sakane, F.
    • Journal Title

      FEBS Open Bio

      Volume: 4 Pages: 387-392

    • DOI

      10.1016/j.fob.2014.04.003

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Regulation of diacylglycerol kinase δ expression in C2C12 skeletal muscle cells by free fatty acids2014

    • Author(s)
      Sakiyama, S., Usuki, T., Sakai, H. and Sakane, F.
    • Journal Title

      Lipids

      Volume: 49 Pages: 633-640

    • DOI

      10.1007/s11745-014-3912-9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Diacylglycerol kinase δ phosphorylates phosphatidylcholine-specific phospholipase C-dependent, palmitic acid-containing diacylglycerol species in response to high glucose levels2014

    • Author(s)
      Sakai, H., Kado, S., Taketomi, A. and Sakane, F.
    • Journal Title

      J. Biol. Chem.

      Volume: 289 Pages: 26607-26617

    • DOI

      10.1074/jbc.m114.590950

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Roles of diacylglycerol kinase isozymes and phosphatidic acid species in neurological functions and disorders.2017

    • Author(s)
      Sakane, F.
    • Organizer
      The 7th International Symposium on Diacylglycerol Kinase
    • Place of Presentation
      Kobe, Hyogo, Japan
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Expression and purification of diacylglycerol kinase α catalytic domain for crystallographic studies.2017

    • Author(s)
      Takahashi, D., Satoh, E. and Sakane, F.
    • Organizer
      The 7th International Symposium on Diacylglycerol Kinase
    • Place of Presentation
      Kobe, Hyogo, Japan
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] New evaluation method of diacylglycerol-generating activity of phosphatidylcholine-specific phospholipase C using mass spectrometry.2017

    • Author(s)
      Murakami, C. and Sakane, F.
    • Organizer
      The 7th International Symposium on Diacylglycerol Kinase
    • Place of Presentation
      Kobe, Hyogo, Japan
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 神経分化誘導時に産生されるジアシルグリセロール及びホスファチジン酸分子種の解析2016

    • Author(s)
      水野悟,坂根郁夫
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      宮城・仙台
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] ジアシルグリセロールキナーゼηノックアウトマウスのそう様行動惹起の分子メカニズム2016

    • Author(s)
      米野井優,坂根郁夫
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      宮城・仙台
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 質量分析法を用いた新規ホスファチジルコリン特異的ホスホリパーゼC活性測定法の開発2016

    • Author(s)
      村上千明,水野悟,坂根郁夫
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      宮城・仙台
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] ジアシルグリセロールキナーゼη欠損によるリチウム感受性躁様行動の誘導2016

    • Author(s)
      陸強,磯崎丈志,米野井優,坂根郁夫
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      宮城・仙台
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] ジアシルグリセロールキナーゼηのプレクストリン相同ドメインの精製とその性質2016

    • Author(s)
      川瀬功暉,坂根郁夫
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      宮城・仙台
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] メラノーマ細胞に対するジアシルグリセロールキナーゼα選択的活性制御化合物の細胞死誘導効果2016

    • Author(s)
      八巻篤実,劉可,国井奈央子,坂根郁夫
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      宮城・仙台
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] C2C12筋芽細胞の筋分化におけるジアシルグリセロールキナーゼδの機能2016

    • Author(s)
      堺弘道,松本健一,坂根郁夫
    • Organizer
      第58回日本脂質生化学会
    • Place of Presentation
      秋田・秋田
    • Year and Date
      2016-06-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] 神経分化誘導時に産生されるジアシルグリセロール及びホスファチジン酸分子種の解析2016

    • Author(s)
      水野悟,坂根郁夫
    • Organizer
      第58回日本脂質生化学会
    • Place of Presentation
      秋田・秋田
    • Year and Date
      2016-06-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] ジアシルグリセロールキナーゼη欠損によるリチウム感受性躁様行動の誘導2016

    • Author(s)
      陸強,磯崎丈志,米野井優,坂根郁夫
    • Organizer
      第58回日本脂質生化学会
    • Place of Presentation
      秋田・秋田
    • Year and Date
      2016-06-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] Diacylglycerol kinase ζ produces 16:0-containing phosphatidic acid molecular species during neuroblastoma cell differentiation.2016

    • Author(s)
      Mizuno, S. and Sakane, F.
    • Organizer
      2016 Annual Meeting of the American Society for Biochemistry and Molecular Biology at Experimental Biology 2016
    • Place of Presentation
      San Diego, California, USA
    • Year and Date
      2016-04-02
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A novel diacylglycerol kinase α-selective inhibitor, CU-3, induces cancer cell apoptosis and enhances immune response2016

    • Author(s)
      Yamaki, A., Liu, Ke., Kunii, N., Mizuno, S. and Sakane, F.
    • Organizer
      Joint Workshop on Chirality in Chiba University and on Soft-Molecule Activation
    • Place of Presentation
      千葉大学(千葉県・千葉市)
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] インスリン抵抗性とジアシルグリセロールキナーゼδ2015

    • Author(s)
      坂根郁夫
    • Organizer
      Biochemistry and Molecular Biology 2015(第88回日本生化学会大会・第38回日本分子生物学会年会)
    • Place of Presentation
      神戸コンベンションセンター(兵庫県・神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 発達中マウスの脳におけるdiacylglycerol kinase δの発現とその脳特異的欠損マウスの表現型解析2015

    • Author(s)
      臼木貴子,高戸珠恵,堺弘道,坂根郁夫
    • Organizer
      Biochemistry and Molecular Biology 2015(第88回日本生化学会大会・第38回日本分子生物学会年会)
    • Place of Presentation
      神戸コンベンションセンター(兵庫県・神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 脂質代謝酵素ジアシルグリセロールキナーゼの多様なモノアシルグリセロールキナーゼ活性2015

    • Author(s)
      佐藤優里子,坂根郁夫
    • Organizer
      Biochemistry and Molecular Biology 2015(第88回日本生化学会大会・第38回日本分子生物学会年会)
    • Place of Presentation
      神戸コンベンションセンター(兵庫県・神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] ジアシルグリセロールキナーゼによって代謝されるジアシルグリセロール分子種のプロテインキナーゼC活性化能2015

    • Author(s)
      神谷侑那, 水野悟, 堺弘道, 坂根郁夫
    • Organizer
      Biochemistry and Molecular Biology 2015(第88回日本生化学会大会・第38回日本分子生物学会年会)
    • Place of Presentation
      神戸コンベンションセンター(兵庫県・神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] ジアシルグリセロールキナーゼηはジアシルグリセロール高親和性のアイソザイムである2015

    • Author(s)
      米野井優,堺弘道,坂根郁夫
    • Organizer
      Biochemistry and Molecular Biology 2015(第88回日本生化学会大会・第38回日本分子生物学会年会)
    • Place of Presentation
      神戸コンベンションセンター(兵庫県・神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] ミリスチン酸は筋管細胞のジアシルグリセロールキナーゼδの発現とグルコース取り込み能を亢進する2015

    • Author(s)
      和田祐子,堺弘道, 崎山静花,坂根郁夫
    • Organizer
      Biochemistry and Molecular Biology 2015(第88回日本生化学会大会・第38回日本分子生物学会年会)
    • Place of Presentation
      神戸コンベンションセンター(兵庫県・神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 神経分化時に産生されるホスファチジン酸分子種とその産生酵素の同定2015

    • Author(s)
      水野悟,坂根郁夫
    • Organizer
      Biochemistry and Molecular Biology 2015(第88回日本生化学会大会・第38回日本分子生物学会年会)
    • Place of Presentation
      神戸コンベンションセンター(兵庫県・神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 精巣に特異的に発現する新規DGKηスプライシングバリアントDGKη3遺伝子のクローニング2015

    • Author(s)
      村上絵梨,塩谷貴生,坂根郁夫
    • Organizer
      Biochemistry and Molecular Biology 2015(第88回日本生化学会大会・第38回日本分子生物学会年会)
    • Place of Presentation
      神戸コンベンションセンター(兵庫県・神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Expression and localization of diacylglycerol kinase δ in the developing mouse brain and phenotype of its brain-specific knockout mice.2015

    • Author(s)
      Usuki, T. and Sakane, F.
    • Organizer
      14th International Conference on Bioactive Lipids in Cancer, Inflammation and Related Diseases
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      2015-07-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Diacylglycerol kinase η is a high affinity isozyme for diacylglycerol.2015

    • Author(s)
      Komenoi, S., Sakai, H. and Sakane, F.
    • Organizer
      14th International Conference on Bioactive Lipids in Cancer, Inflammation and Related Diseases
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      2015-07-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ジアシルグリセロールキナーゼηはジアシルグリセロール高親和性のアイソザイムである2015

    • Author(s)
      米野井優,堺弘道,坂根郁夫
    • Organizer
      第57回日本脂質生化学会
    • Place of Presentation
      東京
    • Year and Date
      2015-05-28 – 2015-05-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 神経分化時に産生されるホスファチジン酸分子種とその産生酵素の同定2015

    • Author(s)
      水野 悟,坂根郁夫
    • Organizer
      第57回日本脂質生化学会
    • Place of Presentation
      東京
    • Year and Date
      2015-05-28 – 2015-05-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] ジアシルグリセロールキナーゼαの選択的活性阻害化合物の同定2015

    • Author(s)
      劉可,国井奈央子,佐藤麻由,白井康仁,坂根郁夫
    • Organizer
      第57回日本脂質生化学会
    • Place of Presentation
      東京
    • Year and Date
      2015-05-28 – 2015-05-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] ジアシルグリセロールキナーゼηはジアシルグリセロール高親和性のアイソザイムである2015

    • Author(s)
      米野井優,堺弘道,坂根郁夫
    • Organizer
      第57回日本脂質生化学会
    • Place of Presentation
      一橋大学一橋講堂(東京都・千代田区)
    • Year and Date
      2015-05-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 神経分化時に産生されるホスファチジン酸分子種とその産生酵素の同定2015

    • Author(s)
      水野 悟,坂根郁夫
    • Organizer
      第57回日本脂質生化学会
    • Place of Presentation
      一橋大学一橋講堂(東京都・千代田区)
    • Year and Date
      2015-05-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] ジアシルグリセロールキナーゼαの選択的活性阻害化合物の同定2015

    • Author(s)
      劉可,国井奈央子,佐藤麻由,白井康仁,坂根郁夫
    • Organizer
      第57回日本脂質生化学会
    • Place of Presentation
      一橋大学一橋講堂(東京都・千代田区)
    • Year and Date
      2015-05-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Diacylglycerol kinase α as an ideal therapeutic target for refractory cancer2015

    • Author(s)
      Sakane, F.
    • Organizer
      88th annual Meeting of the Japanese Pharmacological Society
    • Place of Presentation
      Nagoya
    • Year and Date
      2015-03-18 – 2015-03-20
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 糖尿病性腎症改善薬開発のための新規ジアシルグリセロールキナーゼα活性化剤のスクリーニング2015

    • Author(s)
      林大輝,劉可,上田修司,山之上稔,坂根郁夫,白井康仁
    • Organizer
      第88回日本薬理学会大会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-03-18 – 2015-03-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Diacylglycerol kinase δ phosphorylates phosphatidylcholine-specific phospholipase C-dependent, palmitic acid-containing diacylglycerol species in response to high glucose levels2015

    • Author(s)
      Sakai, H. and Sakane, F.
    • Organizer
      6th International Conference on PLA2 and Lipid Mediators (PLM2015)
    • Place of Presentation
      Tokyo
    • Year and Date
      2015-02-10 – 2015-02-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Expression and Localization of Type II Diacylglycerol Kinase Isozymes δ and η in the Developing Mouse Brain2015

    • Author(s)
      Usuki, T. and Sakane, F.
    • Organizer
      6th International Conference on PLA2 and Lipid Mediators (PLM2015)
    • Place of Presentation
      Tokyo
    • Year and Date
      2015-02-10 – 2015-02-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Diacylglycerol kinases η1 is a high affinity isozyme for diacylglycerol2015

    • Author(s)
      Komenoi, S. and Sakane, F.
    • Organizer
      6th International Conference on PLA2 and Lipid Mediators (PLM2015)
    • Place of Presentation
      Tokyo
    • Year and Date
      2015-02-10 – 2015-02-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 雌雄生殖器における II型ジアシルグリセロールキナーゼアイソザイム, δ, η, κの発現部位の同定と組織内発現分布の解析2014

    • Author(s)
      塩谷貴生,臼木貴子,堺弘道,坂根郁夫
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] II型ジアシルグリセロールキナーゼのプレクストリン相同ドメインとリン脂質の相互作用2014

    • Author(s)
      久米愛子,竹下依那,堺弘道,坂根郁夫
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 発達中マウスの脳におけるII型diacylglycerol kinaseアイソザイムδ及びηの発現と局在2014

    • Author(s)
      臼木貴子,堺弘道,塩谷貴生,坂根郁夫
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] ジアシルグリセロールキナーゼδはホスファチジルコリンに由来するパルミチン酸含有ジアシルグリセロール分子種を選択的にリン酸化する2014

    • Author(s)
      堺弘道,坂根郁夫
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Cold shock-trigger factor系を用いたジアシルグリセロールキナーゼα, δ, ηの発現・精製と活性測定2014

    • Author(s)
      竹村文花,米野井優,佐藤優里子,堺弘道,坂根郁夫
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 神経分化誘導時に産生されるホスファチジン酸およびリゾホスファチジン酸分子種の解析2014

    • Author(s)
      水野悟,堺弘道,坂根郁夫
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] ジアシルグリセロールキナーゼδのモノアシルグリセロールキナーゼ活性2014

    • Author(s)
      藤優理子,堺弘道,水野悟,坂根郁夫
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 脂質代謝酵素ジアシルグリセロールキナーゼδ、ηの酵素学的性質解析2014

    • Author(s)
      米野井優,堺弘道,坂根郁夫
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] ルコース刺激時におけるジアシルグリセロールキナーゼδのパルミチン酸含有ジアシルグリセロール分子種の選択的代謝2014

    • Author(s)
      堺弘道,坂根郁夫
    • Organizer
      第56回日本脂質生化学会
    • Place of Presentation
      東大阪
    • Year and Date
      2014-06-06 – 2014-06-07
    • Related Report
      2014 Annual Research Report
  • [Book] 疾患モデルの作成と利用―脂質代謝異常と関連疾患 下巻2015

    • Author(s)
      坂根郁夫
    • Total Pages
      413
    • Publisher
      エル・アイ・シー
    • Related Report
      2014 Annual Research Report
  • [Remarks] 千葉大学大学院理学研究科 基盤理学専攻 生体機能化学研究室

    • URL

      http://sakane32.wixsite.com/biofunctionchemistry

    • Related Report
      2016 Annual Research Report
  • [Remarks] 千葉大学大学院理学研究科 基盤理学専攻 生体機能化学研究室

    • URL

      http://sakane32.wix.com/biofunctionchemistry

    • Related Report
      2015 Annual Research Report
  • [Remarks] 千葉大学大学院理学研究科基盤理学専攻化学コース生体機能化学研究室

    • URL

      http://biofunction.chem.chiba-u.jp/bfc/Top_Page.html

    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] DGKδノックアウトマウス及びこれを用いた方法2017

    • Inventor(s)
      坂根郁夫
    • Industrial Property Rights Holder
      坂根郁夫
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-004729
    • Filing Date
      2017-01-13
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] DGKηノックアウトマウス及びこれを用いた方法2016

    • Inventor(s)
      坂根郁夫
    • Industrial Property Rights Holder
      坂根郁夫
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-220080
    • Filing Date
      2016-11-10
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2022-02-16  

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