| Project/Area Number |
26291056
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nagoya University |
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Principal Investigator |
Machida Yasunori 名古屋大学, 理学研究科, 研究員(名誉教授) (80175596)
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| Co-Investigator(Kenkyū-buntansha) |
伊藤 正樹 名古屋大学, 生命農学研究科, 准教授 (10242851)
高橋 広夫 金沢大学, 薬学系, 准教授 (30454367)
渡邊 雄一郎 東京大学, 大学院総合文化研究科, 教授 (60183125)
町田 千代子 中部大学, 応用生物学部, 教授 (70314060)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Keywords | Arabidopsis / leaf development / linc RNA / AS2 and AS1 / gene body methylation / DNA methylation / nucleolar bodies / epigenetics / ノンコーディングRNA / AS1-AS2 / 遺伝子発現制御 / 葉分化 / シロイヌナズナ / 発生・分化 / 遺伝子発現 / 小分子 RNA / 遺伝子発現機構 |
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| Outline of Final Research Achievements |
Focusing on long intergenic non-coding (linc) RNAs generated from the promoter region of AS2-target gene ETT, we have attempted to investigate mechanisms of development of adaxial and abaxial domains of leaves in Arabidopsis. However, we failed to amplify the linc RNA molecules by RT-PCR although a molecule was detected at the corresponding position. We also have studied mechanisms of formation of AS2 bodies, which are found at the nucleolar periphery, and supposed to include non-coding RNAs, and its function in leaf development. 1. Our results showed that the zinc-finger-like (ZFL) motif in AS2, which is similar to the zinc finger-CxxC domain in CpG binding proteins of vertebrates, is required for the formation of AS2 bodies. 2. AS2 binds the specific sequence in exon 1 of ETT and the ZFL motif is essential for the AS2 binding. 3. Exon 6 of ETT contains six CpG sites that are fully methylated. AS2 and three nucleolar proteins are involved in maintenance of these methylated CpGs.
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