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Towards genetic modification of non-structural carbohydrate composition in rice stems: Searching for regulatory factors in starch and sucrose metabolisms

Research Project

Project/Area Number 26292009
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Crop production science
Research InstitutionThe University of Tokyo

Principal Investigator

AOKI Naohiro  東京大学, 農学生命科学研究科, 助教 (70466811)

Co-Investigator(Kenkyū-buntansha) 平野 達也  名城大学, 農学部, 教授 (30319313)
廣瀬 竜郎  国立研究開発法人農業・食品産業技術総合研究機構, 中央農業総合研究センター, 主任研究員 (90355579)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywords作物(イネ) / 茎 / 非構造性炭水化物 / デンプン / ショ糖 / アイソジーン / 植物(イネ) / 炭水化物代謝
Outline of Final Research Achievements

In this research project, we tried to identify genes to control the content and composition of non-structural carbohydrates (NSC; mainly starch and sucrose) in the stem of rice plant. As the main results, (1) we identified genes for carbohydrate-related enzymes that act in rice stems: BAM2 and BAM3 for beta-amylases and ISA3 for isoamylase, both of which are starch degrading enzymes, and INV2 for a vacuolar invertase, a sucrose degrading enzyme. Also, we found that (2) the amount and composition of NSC accumulated in the stems at harvest, cannot necessarily be determined by the size of panicle (sink size), and that (3) in the stems of high-NSC cultivars, there exists peculiar mechanism for accumulating high levels of NSC.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (12 results)

All 2017 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (9 results) (of which Invited: 2 results)

  • [Journal Article] Two β-amylase genes, OsBAM2 and OsBAM3, are involved in starch remobilization in rice leaf sheaths2016

    • Author(s)
      Tatsuya Hirano, Takayuki Higuchi, Minako Hirano, Yu Sugimura, Hiroyasu Michiyama
    • Journal Title

      Plant Production Science

      Volume: 19 Issue: 2 Pages: 291-299

    • DOI

      10.1080/1343943x.2016.1140008

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Mutation of the SP1 gene is responsible for the small-panicle trait in the rice cultivar Tachisuzuka, but not necessarily for high sugar content in the stem2016

    • Author(s)
      Tatsuro Hirose, Sakurako Kadoya, Yoichi Hashida, Masaki Okamura, Ryu Ohsugi, Naohiro Aoki
    • Journal Title

      Plant Production Science

      Volume: 253 Issue: 1 Pages: 40-49

    • DOI

      10.1080/1343943x.2016.1260484

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Involvement of α-Amylase Genes in Starch Degradation in Rice Leaf Sheaths at the Post-Heading Stage2015

    • Author(s)
      Yu Sugimura, Hiroyasu Michiyama, Tatsuya Hirano
    • Journal Title

      Plant Production Science

      Volume: 18 Issue: 3 Pages: 277-283

    • DOI

      10.1626/pps.18.277

    • NAID

      130005087242

    • ISSN
      1343-943X, 1349-1008
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] イネ葉鞘において出穂期以降のデンプン分解に関与する遺伝子の探索と機能解析2017

    • Author(s)
      平野達也、杉村優有、樋口貴之
    • Organizer
      日本作物学会第243回講演会
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2017-03-29
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] イネの茎部低デンプン変異体を利用した茎部炭水化物蓄積特性の解明2017

    • Author(s)
      岡村昌樹、廣瀬竜郎、橋田庸一、大杉立、青木直大
    • Organizer
      日本作物学会第243回講演会
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2017-03-29
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] イネ葉鞘におけるデンプン含量の変化とα-グルカンホスホリラーゼ活性との関係2016

    • Author(s)
      樋口貴之、道山弘康、平野達也
    • Organizer
      日本作物学会第242回講演会
    • Place of Presentation
      龍谷大学(滋賀県大津市)
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] β-アミラーゼ遺伝子, OsBAM5の発現抑制系統はイネ葉鞘においてデンプン過剰の表現型を示す2015

    • Author(s)
      杉村優有、平野美奈子、道山弘康、深山浩、平野達也
    • Organizer
      日本作物学会第240回講演会
    • Place of Presentation
      信州大学(長野県長野市)
    • Year and Date
      2015-09-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] イネのショ糖リン酸合成酵素をコードするOsSPS1の発現が抑制された変異体の生理学的解析2015

    • Author(s)
      橋田庸一、廣瀬竜郎、青木直大、大杉立
    • Organizer
      日本作物学会第240回講演会
    • Place of Presentation
      信州大学(長野県長野市)
    • Year and Date
      2015-09-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] Characterization of vacuolar invertase genes OsINV2 and OsINV3 in rice (Oryza sativa L.)2015

    • Author(s)
      Shamitha Rao Morey、廣瀬竜郎、青木直大、大杉立
    • Organizer
      日本作物学会第240回講演会
    • Place of Presentation
      信州大学(長野県長野市)
    • Year and Date
      2015-09-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] イネ葉身でのデンプン含量の日変化におけるβ‐アミラーゼの関与2015

    • Author(s)
      杉村優有、道山弘康、平野達也
    • Organizer
      日本作物学会第239回講演会
    • Place of Presentation
      日本大学湘南キャンパス
    • Year and Date
      2015-03-27 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 茎部のデンプン合成が抑制されたイネ変異体の糖蓄積に関する研究2014

    • Author(s)
      太田黒駿、青木直大、岡村昌樹、廣瀬竜郎、大杉立
    • Organizer
      日本作物学会第238回講演会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Year and Date
      2014-09-09 – 2014-09-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] イネにおけるIsoamylase3の欠失変異体の解析2014

    • Author(s)
      辻本翔大、青木直大、廣瀬竜郎、大杉立
    • Organizer
      日本作物学会第238回講演会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Year and Date
      2014-09-09 – 2014-09-10
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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