Researches for the pathogenesis and disease models of canine degenerative myelopathy, leptomeningeal histiocytic sarcoma, and multiple myositis.

• Project/Area Number: 26292157
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: The University of Tokyo
• Principal Investigator: UCHIDA Kazuyuki 東京大学, 大学院農学生命科学研究科(農学部), 准教授 (10223554)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: コーギー犬 / 組織球性肉腫 / Cell line / 樹状細胞 / マクロファージ / ランゲルハンス細胞 / 変性性脊髄症 / 筋疾患 / 炎症性筋症 / ウェルシュコーギー / 犬 / 神経原性筋疾患 / 筋原性筋疾患 / 筋炎 / イヌ / 炎症性筋疾患 / ウェルシュ・コーギー / 多発性筋炎 / 筋萎縮性側索硬化症 / SOD1

Outline of Final Research Achievements

The present study revealed the pathological features of breed specific diseases occurring in Pembroke Welsh Corgi(PWC) dogs, especially histiocytic sarcoma and degenerative myelopathy. Regarding histiocytic sarcomas, cell line derived from leptomeningealhistiocytic sarcomas in a PWC dog was established. Two additional cell lines from synovial histiocytic sarcoma of a flat-coated retriever dog and canine cutaneous Langerhans cell histiocytosis have been successfully established. These three types of cell lines will be exellent tools for the basic researches on tumoregenesis and therapeutic drug development on histiocytic sarcomas, not only in dogs but also in humans. Regarding canine degenerative myelopathy, autophagy abnormality is involved in addition to SOD1 mutation in PWC dogs.

Research Products

• [Journal Article] Immunohistochemical analysis of canine and feline muscle disorders using formalin-fixed, paraffin-embedded tissues. (2017)
  • Author(s): Shiga T, Uchida K, Chambers JK, Nakayama H.
  • Journal Title: Journal of Veterinary Diagnostic Investigation Volume: 29(6): Pages: 805-813
  • DOI: 10.1177/1040638717715287
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Variations in Histiocytic Differentiation of Cell Lines From Canine Cerebral and Articular Histiocytic Sarcomas. (2017)
  • Author(s): Thongtharb A, Uchida K, Chambers JK, Nakayama H.
  • Journal Title: Veterinary Pathology Volume: In press Pages: 395-404
  • DOI: 10.1177/0300985817690211
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Histological and immunohistochemical features of histiocytic sarcoma in four domestic ferrets (Mustela putorius furo) (2016)
  • Author(s): Thongtharb A, Uchida K, Chambers JK, Miwa Y, Murata Y, Nakayama H
  • Journal Title: J Vet Diagn Invest. Volume: 28 Pages: 165-170
  • DOI: 10.1177/1040638715626485
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Histological and immunohistochemical studies on primary intracranial canine histiocytic sarcomas (2016)
  • Author(s): Thongtharb A, Uchida K, Chambers JK, Kagawa Y, Nakayama H
  • Journal Title: J. Vet. Med. Sci. Volume: in press Issue: 4 Pages: 593-599
  • DOI: 10.1292/jvms.15-0627
  • NAID: 130007331496
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Expression of autophagy-related proteins in the spinal cord of pembroke welsh corgi dogs with canine degenerative myelopathy. (2015)
  • Author(s): Ogawa M, Uchida K, Yamato O, Mizukami K, Chambers JK, Nakayama H.
  • Journal Title: Vet Pathol Volume: 未定 Pages: 1099-1107
  • DOI: 10.1177/0300985815570070
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Expression of autophagy-related proteins in the spinal cord of Pembroke Welsh Corgi dogs with canine degenerative myelopathy. (2015)
  • Author(s): Ogawa M, Uchida K, Yamato O, Mizukami K, Chambers JK, Nakayama H.
  • Journal Title: Vet Pathol. Volume: In press
  • Peer Reviewed / Open Access
• [Journal Article] Clinical Characteristics and Prognostic Factors in Dogs with Histiocytic Sarcomas in Japan (2014)
  • Author(s): Takahashi M1, Tomiyasu H, Hotta E, Asada H, Fukushima K, Kanemoto H, Fujino Y, Ohno K, Uchida K, Nakayama H, Tsujimoto H.
  • Journal Title: J. Vet. Med. Sci. Volume: In press Issue: 5 Pages: 661-666
  • DOI: 10.1292/jvms.13-0414
  • NAID: 130003391271
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Presentation] Pathological study on primary leptomeningeal histiocytic sarcoma in dogs. (2015)
  • Author(s): A. Thongtharb, K. Uchida, J.K. Chambers, and H. Nakayama
  • Organizer: 2015 ACVP/ASVCP/STP Combined Annual Meeting
  • Place of Presentation: ミネアポリス、ミネソタ州、アメリカ
  • Year and Date: 2015-10-17
  • Int'l Joint Research