Limit break of photosynthesis by improvement of RuBisCO functions
| Project/Area Number |
26292193
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kobe University |
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Principal Investigator |
Ashida Hiroki 神戸大学, 人間発達環境学研究科, 准教授 (50362851)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Keywords | 光合成 / CO2固定 / ルビスコ / アーキア / シアノバクテリア / 進化 / ルビスコアクチベース / 植物 |
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| Outline of Final Research Achievements |
We found methanogenic archaeal RuBisCO functions in the novel CO2 fixation pathway which has some different steps from the photosynthetic Calvin cycle, suggesting that this novel pathway should be the ancient Calvin cycle. Enzymatic analysis of hybrid RuBisCO created by swap of large and small subunits between thermophilic cyanobacteria RuBisCO with high CO2 specificity and mesophilic cyanobacteria RuBisCO revealed that small subunit was involved in CO2 specificity. Transplastomic tobacco with over expression of RuBisCO activase tends to be enhanced a photosynthetic CO2 fixation rate.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] A RuBisCO-mediated carbon metabolic pathway in methanogenic archaea.2017
Author(s)
T. Kono, S. Mehrotra, C. Endo, N. Kizu, M. Matsuda, H. Kimura, E. Mizohata, T. Inoue, T. Hasunuma, A. Yokota, H. Matsumura, H. Ashida
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Journal Title
Nat Commun
Volume: 8
Pages: 14007-14007
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] 多彩な戦略で挑むシアノバクテリア由来の燃料生産2017
Author(s)
日原由香子, 朝山宗彦, 蘆田弘樹, 天尾豊, 新井宗仁, 粟井光一郎, 得平茂樹, 小山内崇, 鞆達也, 成川礼, 蓮沼誠久, 増川一
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Journal Title
化学と生物
Volume: 55
Pages: 88-97
Related Report
Peer Reviewed
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[Presentation] 光合成機能の研究と応用2016
Author(s)
蘆田弘樹
Organizer
日本品質管理学会
Place of Presentation
大阪大学中之島センター（大阪府・大阪市）
Year and Date
2016-09-02
Related Report
Invited
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