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Structure and dynamics of intermediate filament proteins

Research Project

Project/Area Number 26293038
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Terada Sumio  東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (00262022)

Co-Investigator(Kenkyū-buntansha) 川岸 将彦  東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (60323606)
齊藤 健太  東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (60374659)
佐藤 啓介  東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (60644044)
Co-Investigator(Renkei-kenkyūsha) FUKUMA Takeshi  金沢大学, 理工学部, 教授 (90452094)
UESUGI Motonari  京都大学, 物質-細胞統合システム拠点, 教授 (10402926)
Research Collaborator TANI Tomomi  米国ウッズホール海洋生物学研究所, 研究員
SATO Fumiya  東京医科歯科大学, 大学院・医歯学総合研究科, 大学院学生
TAGUCHI Mie  東京医科歯科大学, 大学院・医歯学総合研究科, 医療技術職員
YUASA Mari  東京医科歯科大学, 生体材料工学研究所, 特任助教
KASAMA Kenji  東京医科歯科大学, 医歯学研究支援センター, 准教授
KOHDA Takashi  東京医科歯科大学, 難治疾患研究所, 准教授
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Keywords細胞骨格 / 中間径フィラメント / 細胞学
Outline of Final Research Achievements

In this study, we applied the modified ‘cells on glass sandwich’ method to exteriorize intracellular neurofilaments, reducing the risk of causing artefacts through sample preparation. The observed thin filaments, considered to retain native structures of the neurofilaments, exhibited an approximate periodicity of 50-60 nm along their length. Our success of semi- in situ atomic force microscopy of exposed bona fide assembled neurofilaments through separating the sandwich suggests that it can be an effective and alternative method for investigating cytoplasmic intermediate filaments under physiological conditions by atomic force microscopy. In addition, we could develop new methods to label cytoskeletal proteins for fluorescent polarization microscopic analysis.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (10 results)

All 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (1 results)

  • [Int'l Joint Research] ウッズホール海洋生物学研究所(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] ウッズホール海洋生物学研究所(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Semi-in situ atomic force microscopy imaging of intracellular neurofilaments under physiological conditions through the ‘sandwich’ method2016

    • Author(s)
      Fumiya Sato, Hitoshi Asakawa, Takeshi Fukuma, and Sumio Terada
    • Journal Title

      Microscopy (Oxf)

      Volume: - Issue: 4 Pages: 316-324

    • DOI

      10.1093/jmicro/dfw006

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] PANDIA:a novel marker for fluorescence polarization microscopy of actin dynamics in living cells2017

    • Author(s)
      佐藤文哉ほか
    • Organizer
      第122回日本解剖学会全国学術集会
    • Place of Presentation
      長崎大学医学部(長崎県長崎市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Semi-in-situ Atomic Force Microscopy Imaging of Intracellular Neurofilaments.2016

    • Author(s)
      Fumiya Sato,Hitoshi Asakawa,Takeshi Fukuma,Sumio Terada.
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] Semi- in situ atomic force microscopy imaging of intracellular neurofilaments under physiological conditions through the ‘sandwich’ method2016

    • Author(s)
      佐藤文哉ほか
    • Organizer
      第:39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-07-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] Semi- in situ atomic force microscopy imaging of intracellular neurofilaments under physiological conditions through the ‘sandwich’ method2016

    • Author(s)
      佐藤文哉ほか
    • Organizer
      第121回日本解剖学会全国学術集会
    • Place of Presentation
      ビッグパレットふくしま(福島県郡山市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Close-up pictures of cytoskeletal dynamics in neurons.2016

    • Author(s)
      Terada S.
    • Organizer
      The 7th Asia Pacific International Congress of Anatomists
    • Place of Presentation
      Singapore (Singapore)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Microscopic and spectroscopic dissections of cytoskeletal protein dynamics in neurons.2016

    • Author(s)
      Terada S.
    • Organizer
      Seminar, CAM-SU Genomic Research Center, Soochow University, China
    • Place of Presentation
      Soochow (China)
    • Year and Date
      2016-01-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Remarks] 研究室紹介ウェッブページ

    • URL

      http://www.tmd.ac.jp/cgi-bin/nana/index.cgi

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report

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Published: 2014-04-04   Modified: 2022-06-09  

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