Dissecting tolerance and resistance mechanism regulating vectorial competency of pathogen-transmitting vectors

• Project/Area Number: 26293092
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Jikei University School of Medicine
• Principal Investigator: KANUKA HIROTAKA 東京慈恵会医科大学, 医学部, 教授 (50342770)
• Co-Investigator(Kenkyū-buntansha): 熊谷 正広 東京慈恵会医科大学, 医学部, 講師 (00271304) ; 案浦 健 国立感染症研究所, その他部局等, 研究員 (90407239)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000); Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: トレランス / レジスタンス / 蚊 / 感染症 / 節足動物 / ウイルス / 免疫応答 / 病原体媒介節足動物 / 感染 / コンピテンシー / 病原体 / 中間宿主 / 寄生虫 / 細菌 / 衛生動物 / 免疫

Outline of Final Research Achievements

Blood-sucking arthropods, including mosquitoes transmit numerous virus diseases during blood feeding. Using a virus infection model in Drosophila and Aedes mosquito, we uncovered genetically encoded resistance of vector mosquito to virus infection as measured by the extent to which survival rate decreased via increasing virus burden. These results suggest that anti-virus responses of vector mosquito are not restricted to its survival but rather can be applied to capacity to employ virus mutualism during the disease transmission.

Research Products

• [Int'l Joint Research] ジョセフ・キゼルボ教授記念ワガ第一大学(ブルキナファソ)
• [Int'l Joint Research] 國家衛生研究院(台湾)
• [Int'l Joint Research] ワガドゥグ大学/国立マラリア研究・研修センター(ブルキナファソ)
• [Int'l Joint Research] イバダン大学(ナイジェリア)
• [Int'l Joint Research] 国家衛生研究院(台湾)
• [Journal Article] Proboscis infection route of Beauveria bassiana triggers early death of Anopheles mosquito (2017)
  • Author(s): Minehiro Ishii, Hirotaka Kanuka, Athanase Badolo, N'Fale Sagnon, Wamdaogo Guelbeogo, Masanori Koike, and Daigo Aiuchi
  • Journal Title: Scientific Reports Volume: 印刷中
  • NAID: 120006540767
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Interaction between tapeworm and coleopteran intermediate host (2014)
  • Author(s): Ayako Hyuga, Takuya Yokoyama, Hirotaka Kanuka
  • Journal Title: Jpn. J. Vet. Parasitol. Volume: 13 Pages: 86-90
  • Peer Reviewed
• [Presentation] Generation of transgenic mosquito harboring pseudotype virus (2017)
  • Author(s): 嘉糠洋陸
  • Organizer: 2017 International conference on Dengue and Dengue prevention
  • Place of Presentation: 台湾（台南）
  • Year and Date: 2017-03-07
  • Int'l Joint Research / Invited
• [Presentation] トランスジェニック蚊を用いたウイルス生ワクチン産生の試み (2016)
  • Author(s): 嘉糠洋陸
  • Organizer: 第２4回分子寄生虫学ワークショップ／第１4回分子寄生虫・マラリア研究フォーラム合同大会
  • Place of Presentation: 帯広
  • Year and Date: 2016-08-21
• [Presentation] マダニ刺咬症と感染症のリスクを知る (2016)
  • Author(s): 嘉糠洋陸
  • Organizer: 第115回日本皮膚科学会
  • Place of Presentation: 京都
  • Year and Date: 2016-06-03
  • Invited
• [Presentation] The role of gut in pathogen-transmitting insects: barrier or gate? (2016)
  • Author(s): 嘉糠洋陸
  • Organizer: The 1st International Symposium for Infectious Diseases: Vector-borne Diseas
  • Place of Presentation: 中国（深セン）
  • Year and Date: 2016-05-31
  • Int'l Joint Research / Invited
• [Presentation] 重症熱性血小板減少症候群(SFTS)ウイルスのマダニ内伝播メカニズムの解析 (2016)
  • Author(s): 山地佳代子、下島昌幸、西條政幸、嘉糠洋陸
  • Organizer: 第68回日本衛生動物学会
  • Place of Presentation: 栃木
  • Year and Date: 2016-04-15
• [Presentation] Insect and pathogen interaction: friend or enemy? (2015)
  • Author(s): 嘉糠洋陸
  • Organizer: 第5回モデル生物丸ごと一匹学会
  • Place of Presentation: 大阪
  • Year and Date: 2015-12-19
  • Invited
• [Presentation] The role of gut in pathogen-transmitting mosquito: barrier or gate? (2015)
  • Author(s): 嘉糠洋陸
  • Organizer: 第36回台湾昆虫学会
  • Place of Presentation: 台湾
  • Year and Date: 2015-10-30
  • Int'l Joint Research / Invited
• [Presentation] 中間宿主甲虫の小形条虫に対する感染コンピテンシー (2015)
  • Author(s): 横山卓也、池田愛、浅野和仁、渡邊直煕、友安慶典、嘉糠洋陸
  • Organizer: 第２３回分子寄生虫学ワークショップ／第１３回分子寄生虫・マラリア研究フォーラム合同大会
  • Place of Presentation: 帯広
  • Year and Date: 2015-08-30
• [Presentation] Non blood-sucking vectors: Odor-based mechanical transmission of bacteria by fly feces (2015)
  • Author(s): 岡戸清、佐久間知佐子、山地佳代子、青沼宏佳、嘉糠洋陸
  • Organizer: EMBO国際会議Molecular and population biology of mosquitoes and other disease vectors
  • Place of Presentation: ギリシャ
  • Year and Date: 2015-07-24
  • Int'l Joint Research
• [Presentation] Odor-based mechanical transmission of bacteria by fly feces (2015)
  • Author(s): 岡戸清、佐久間知佐子、山地佳代子、青沼宏佳、嘉糠洋陸
  • Organizer: Keystone国際会議The Arthropod Vector: The Controller of Transmission
  • Place of Presentation: 米国
  • Year and Date: 2015-05-12
  • Int'l Joint Research
• [Presentation] Evidence of vertical transmission of severe fever with thrombocytopenia syndrome virus in field-collected ticks (2015)
  • Author(s): 山地佳代子、下島昌幸、西條政幸、青沼宏佳、嘉糠洋陸
  • Organizer: Keystone国際会議The Arthropod Vector: The Controller of Transmission
  • Place of Presentation: 米国
  • Year and Date: 2015-05-12
  • Int'l Joint Research
• [Presentation] 中間宿主甲虫の小形条虫に対する感染コンピテンシー (2015)
  • Author(s): 横山卓也、日向綾子、山地佳代子、浅野和仁、渡邊直煕、友安慶典、嘉糠洋陸
  • Organizer: 第84回日本寄生虫学会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] マダニ媒介性感染症～感染症が運ばれるリスクを知る～ (2014)
  • Author(s): 嘉糠洋陸
  • Organizer: 第63回感染症学会東日本地方会
  • Place of Presentation: 東京
  • Year and Date: 2014-10-29 – 2014-10-31
  • Invited
• [Presentation] The role of gut in pathogen-transmitting insects: barrier or gate? (2014)
  • Author(s): 嘉糠洋陸
  • Organizer: 第87回日本生化学会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
  • Invited
• [Book] 蚊はなぜ人を襲うのか (2016)
  • Author(s): 嘉糠洋陸
  • Total Pages: 128
  • Publisher: 岩波書店