Development of a novel oncolytic virus by regulating the processing of virus-derived non-coding RNA
| Project/Area Number |
26293118
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied pharmacology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MIZUGUCHI HIROYUKI 大阪大学, 大学院薬学研究科, 教授 (50311387)
TACHIBANA MASASHI 大阪大学, 大学院薬学研究科, 特任准教授 (80513449)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Keywords | アデノウイルス / 非コードRNA / 腫瘍溶解性ウイルス / Dicer / 腫瘍溶解性アデノウイルス / 癌 / microRNA / プロセシング |
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| Outline of Final Research Achievements |
An adenovirus (Ad) genome encodes two non-coding RNAs, VA-RNA I and VA-RNA II. Previous studies demonstrated that VA-RNAs enhance Ad infection and that Dicer processes VA-RNAs, producing miRNA-like small RNAs (mivaRNA); however, relationship between Dicer-mediated processing of VA-RNAs and VA-RNA-mediated enhancement of Ad infection remained to be elucidated. In this study, we examined the effects of Dicer-mediated processing of VA-RNAs on Ad infection. We demonstrated that Dicer-mediated proessing of VA-RNA I resulted in loss of promotion activity of VA-RNA I for Ad infection. Based on these findings, we developed a novel oncolytic Ad showing superior antitumor effects.
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Report
(5 results)
Research Products
(52 results)
