Development of an innovative lymphocyte chip to establish personalized immuno-therapy for infectious diseases
Project/Area Number |
26293237
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Infectious disease medicine
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Research Institution | University of Toyama |
Principal Investigator |
MURAGUCHI Atsushi 富山大学, 大学院医学薬学研究部(医学), 教授 (20174287)
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Co-Investigator(Kenkyū-buntansha) |
岸 裕幸 富山大学, 大学院医学薬学研究部(医学), 准教授 (60186210)
小澤 龍彦 富山大学, 大学院医学薬学研究部(医学), 助教 (10432105)
浜名 洋 富山大学, 大学院医学薬学研究部(医学), 助教 (90551549)
小林 栄治 富山大学, 大学院医学薬学研究部(医学), 助教 (70459733)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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Keywords | リンパ球チップ / Tリンパ球 / T細胞受容体 / 免疫治療 / 個の免疫 / Tリンパ球 / T細胞受容体 |
Outline of Final Research Achievements |
To develop an innovative lymphocyte chip to establish personalized immuno-therapy for infectious diseases, 1) We developed a new lymphocyte chip using microwave-coated chip that were able to efficiency trap single microwave-beads coated lymphocytes. 2) We succeeded in retrieving a single antigen-specific T lymphocytes from lymphocyte chip and efficiently analyzing TCR gene sequences of alpha- and beta-TCR gene. 3) We succeeded in detection of antigen-specific CD4-positive T lymphocytes using lymphocyte chip. 4) We improved a method for verifying TCR function using various target cell lines. 5) We successfully constructed sTCR ( a dimer containing TCR V-region and TCR C-region) and scFv-TCR ( a monomer containing V-alpha gene and V-beta gene in a single polypeptide). 6) We established 4 kinds of human anti-West Nile virus monoclonal antibodies with neutralizing activities.
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Report
(4 results)
Research Products
(66 results)
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[Journal Article] Association Between High-Avidity T-Cell Receptors, Induced by α-Fetoprotein-Derived Peptides, and Anti-Tumor Effects in Patients With Hepatocellular Carcinoma2017
Author(s)
Nakagawa H, Mizukoshi E, Kobayashi E, Tamai T, Hamana H, Ozawa T, Kishi H, Kitahara M, Yamashita T, Arai K, Terashima T, Iida N, Fushimi K, Muraguchi A, Kaneko S
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Journal Title
Gastroenterology
Volume: 152
Issue: 6
Pages: 1395-1406
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Identification of an HLA class I allele closely involved in the auto-antigen presentation in acquired aplastic anemia2017
Author(s)
Zaimoku Y, Takamatsu H, Hosomichi K, Ozawa T, Nakagawa N, Imi T, Maruyama H, Katagiri T, Kishi H, Tajima A, Muraguchi A, Kashiwase K, Nakao S
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Journal Title
Blood
Volume: 印刷中
Issue: 21
Pages: 2908-2916
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Fully human monoclonal antibodies to TRAIL-R1 enhance TRAIL-induced apoptosis via activation of caspase-8 pathway.2016
Author(s)
Hao Z, Han X, Sun X, Shen M, Huang J, Li Y, Ozawa T, Pang D, Jin S, Kishi H, Muraguchi A, Jin A.
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Journal Title
Biochem Biophys Res Commun.
Volume: 475
Issue: 2
Pages: 238-44
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Identification of broadly conserved cross-species protective Leishmania antigen and its responding CD4 + T cells2015
Author(s)
Z. Mou, J. Li, T. Boussoffara, H. Kishi, H. Hamana, P. Ezzati, C. Hu, W. Yi, D. Liu, F. Khadem, I. Okwor, P. Jia, K. Shitaoka, S. Wang, M. Ndao, C. Petersen, J. Chen, S. Rafati, H. Louzir, A. Muraguchi, J. a Wilkins, and J. E. Uzonna,
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Journal Title
Science Translational Medicine
Volume: 7
Issue: 310
Pages: 1-13
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Immunological features of T cells induced by human telomerase reverse transcriptase-derived peptides in patients with hepatocellular carcinoma.2015
Author(s)
E Mizukoshi, H Nakagawa, M Kitahara, T Yamashita, K Arai, H Sunagozaka, K Fushimi, E Kobayashi, H Kishi, A Muraguchi, S Kaneko.
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Journal Title
Cancer Lett
Volume: 364
Issue: 2
Pages: 98-105
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay2015
Author(s)
Takeda H, Ogasawara T, Ozawa T, Muraguchi A, Jih PJ, Morishita R, Uchigashima M, Watanabe M, Fujimoto T, Iwasaki T, Endo Y, Sawasaki T
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Journal Title
Sci Rep
Volume: 5
Issue: 1
Pages: 11333-11333
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Monoclonal Antibody Against Citrullinated Peptides Obtained From Rheumatoid Arthritis Patients Reacts With Numerous Citrullinated Microbial and Food Proteins2015
Author(s)
Reina Tsuda, Tatsuhiko Ozawa, Eiji Kobayashi, Hiroshi Hamana, Hirofumi Taki, Kazuyuki Tobe, Eiji Sugiyama, Masahiro Iwamoto, Johji Imura, Hiroyuki Kishi, Atsushi Muraguchi
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Journal Title
Arthritis & Rheumatology
Volume: 67
Issue: 8
Pages: 2020-2031
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Cis-interaction of TCRs and antigen/MHC class I complexes on CD8+ T cells causes their activation.2016
Author(s)
Kishi H, Jin A, Hamana H, Shitaoka K, Tajiri K, Kobayashi E, Kusano S, Yokoyama S, Ozawa T, Nagai T, Obata T, Hatakeyama S, Horii M, Hu Y, Zhang F, Muraguchi A.
Organizer
2016 International Congress of Immunology
Place of Presentation
Melbourne(豪州)
Year and Date
2016-08-21
Related Report
Int'l Joint Research
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[Presentation] Comprehensive T-Cell Receptor Repertoire Analysis Using Deep Sequencing and Single Cell Cloning Reveals Extreme Oligoclonality of Ex Vivo Expanded Cytomegalovirus-Reactive Cytotoxic T-Cells.2015
Author(s)
Miyama T, Kawase T, Kitaura K, Chishaki R, Shibata M, Oshima K, Hamana H, Kishi H, Kuzushima K, Saji H, Suzuki R, Ichinohe T.
Organizer
The 57th ASH Annual Meeting and Exposition
Place of Presentation
Orlando(米国)
Year and Date
2015-12-05
Related Report
Int'l Joint Research
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