| Project/Area Number |
26293414
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Prosthodontics/ Dental materials science and
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小戝 健一郎 鹿児島大学, 医歯学域医学系, 教授 (90258418)
朝比奈 泉 長崎大学, 医歯(薬)学総合研究科, 教授 (30221039)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | 再生医療 / 間葉系幹細胞 / 骨再生 / 骨増生 / 顎骨増生 / 血管再生 / 顎堤増生 |
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| Outline of Final Research Achievements |
In the present study, we succeeded in development of a novel method to construct cell/scaffold complex using the sodium alginate. The uniformity and operativity of the cell/scaffold complex was apparently improved by using this novel method. Next, we constructed the mesenchymal stem cells (MSCs)/scaffold complex using sodium alginate, and then transplanted them on the mice calvariae. At 4 weeks after surgery, newly formed bone was confirmed in the grafts. Furthermore, neovessel and neolymphvessel formation was induced in the grafts area. VEGF-C is known to stimulate lymphangiogenesis, so, we next investigated whether VEGF-C modulates osteogenic differentiation of MSCs. Treatment with VEGF-C significantly induced the osteogenic differentiation of MSCs. In addition, we also revealed that treatment with VEGF-C significantly enhanced MSCs migration. These results indicate that not only angiogenesis but lymphangiogenesis may play an important role in bone formation process.
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