Development of genetic marker for artemisinin-resistant malaria: Field-genomics analysis

• Project/Area Number: 26305015
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Juntendo University
• Principal Investigator: Mita Toshihiro 順天堂大学, 医学(系)研究科(研究院), 教授 (80318013)
• Co-Investigator(Kenkyū-buntansha): 大橋 順 東京大学, 大学院理学系研究科(理学部), 准教授 (80301141)
• Project Period (FY): 2014-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: マラリア / アルテミシニン / 薬剤耐性 / 遺伝子マーカー / 早期診断 / 分子疫学 / 耐性進化 / K13 / Kelch13 / 分子マーカー / 耐性

Outline of Final Research Achievements

The aim of this research is to develop genetic maker(s) that is useful for the containment strategy of resistant parasites. We achieved, 1) first identification of artemisinin-resistant parasites in Africa and identification of potential resistant marker, and 2) detection of malaria parasites harboring mutant PfKelch13 allele (C580Y) and elucidation of its origin and route of their spread. These achievement would greatly contribute the practical application toward the surveillance of resistant parasites.

Academic Significance and Societal Importance of the Research Achievements

マラリアは世界３大感染症の一つである。その根絶のために抗マラリア薬は不可欠であるが、容易に耐性化する。本研究でアフリカでの有用性が期待される新規候補マーカーが明らかになった。今後のさらなる検討によって、その有用性が確認されれば、今後はアフリカにおける耐性原虫の出現と拡散を簡便に評価することが可能になる。これは、耐性マラリア原虫の封じ込め対策へ大きく貢献する。

Research Products

• [Int'l Joint Research] グル大学/St Mary Hospital, Lacor(ウガンダ)
• [Int'l Joint Research] デバイン大学/ウエワク総合病院(パプアニューギニア)
• [Int'l Joint Research] オックスフォード大学/サンガー研究所(英国)
• [Int'l Joint Research] マヒドン大学(タイ)
• [Int'l Joint Research] オックスフォード大学(英国)
• [Int'l Joint Research] マヒドン大学(タイ)
• [Int'l Joint Research] グル大学/St.Mary Lacor病院(ウガンダ)
• [Int'l Joint Research] デバインワード大学(パプアニューギニア)
• [Int'l Joint Research] グル大学/Lacor病院(Uganda)
• [Int'l Joint Research] デバイン大学(Papua New Guinea)
• [Int'l Joint Research] 国際保健研究所(ミャンマー)
• [Journal Article] 【微生物と人類の闘いと共存】 薬剤耐性マラリア原虫の進化 第一選択薬アルテミシニンへの耐性メカニズムから紐解く進化と適応. (2018)
  • Author(s): 美田敏宏
  • Journal Title: 生物の科学 Volume: 遺伝: Pages: 382-8
• [Journal Article] 薬剤耐性マラリアの最新疫学知見 (2018)
  • Author(s): 美田敏宏
  • Journal Title: 病原微生物検出情報 Volume: 1 Pages: 173-4
• [Journal Article] Lack of significant recovery of chloroquine sensitivity in Plasmodium falciparum parasites following discontinuance of chloroquine use in Papua New Guinea (2018)
  • Author(s): Sekihara M, Tachibana S-I, Yamauchi M, Yatsushiro S, Tiwara S, Fukuda N, Ikeda M, Mori T, Hirai M, Hombhanje F, Mita T
  • Journal Title: Malar J. Volume: 17 Pages: 434-434
  • DOI: 10.1186/s12936-018-2585-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Human migration and the spread of malaria parasites to the New World. (2018)
  • Author(s): Rodrigues PT, et al 15/25.
  • Journal Title: Scientific reports. Volume: 8 Pages: 1993-1993
  • DOI: 10.1038/s41598-018-19554-0
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Rapid selection of sulphadoxine-resistant Plasmodium falciparum and its effect on within-population genetic diversity in Papua New Guinea. (2018)
  • Author(s): Mita T, Hombhanje F, Takahashi N, Sekihara M, Yamauchi M, Tsukahara T, Kaneko A, Endo H, Ohashi J. (Corresponding author)
  • Journal Title: Scientific Reports Volume: 8 Pages: 5565-5565
  • DOI: 10.1038/s41598-018-23811-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Artemisinin-Resistant Plasmodium falciparum with High Survival Rates, Uganda, 2014-2016 (2018)
  • Author(s): Ikeda M, Kaneko M, Tachibana S-I, Balikagala B, Sakurai-Yatsushiro M, Yatsushiro S, Takahashi N, Yamauchi M, Sekihara M, Hashimoto M, Katuro OT, Olia A, Obwoya PS, Auma MA, Anywar DA, Odongo-Aginya EI, Okello-Onen J, Hirai M, Ohashi J, Palacpac NMQ, Kataoka M, Tsuboi T, Kimura E, Horii T, Mita T.
  • Journal Title: Emerging infectious diseases Volume: 24 Pages: 718-26
  • DOI: 10.3201/eid2404.170141
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 【今、蚊を考える-蚊媒介感染症に関する最近の話題】 蚊媒介感染症に関する最近の話題 マラリア (2017)
  • Author(s): 美田敏宏
  • Journal Title: 臨床と微生物 Volume: マラリア Pages: 263-8
• [Journal Article] Absence of in vivo selection for K13 mutations after artemether-lumefantrine treatment in Uganda. (2017)
  • Author(s): Balikagala B, Mita T, Ikeda M, Sakurai M, Yatsushiro S, Takahashi N, Tachibana SI, Auma M, Ntege EH, Ito D, Takashima E, Palacpac NM, Egwang TG, Onen JO, Kataoka M, Kimura E, Horii T, Tsuboi T.
  • Journal Title: Malaria Journal Volume: 16(1) Pages: 1-23
  • DOI: 10.1186/s12936-016-1663-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Absence of in-vivo selection for K13 mutations after artemether-lumefantrine treatment in Uganda. (2017)
  • Author(s): Balikagala B, Mita T*, Ikeda M, Sakurai M, Yatsushiro S, Takahashi N, Tachibana SI, Auma M, Ntege EH, Ito D, Takashima E, Palacpac NM, Egwang TG, Ohashi J, Kataoka M, Kimura E, Horii T and Tsuboi T.
  • Journal Title: Malaria J Volume: 16 Pages: 23-23
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Honma H, Niikura M, Kobayashi F, Horii T, Mita T, Endo H and Hirai M. (2016)
  • Author(s): Honma H, Niikura M, Kobayashi F, Horii T, Mita T, Endo H and Hirai M.
  • Journal Title: Sci Rep Volume: 6 Pages: 36971-36971
  • Peer Reviewed
• [Journal Article] Household clustering of asymptomatic malaria infections in Xepon district, Savannakhet province, Lao PDR. (2016)
  • Author(s): Pongvongsa T, Nonaka D, Iwagami M, Nakatsu M, Phongmany P, Nishimoto F, Kobayashi J, Hongvanthon B, Brey PT, Moji K, Mita T, Kano S.
  • Journal Title: Malar J. Volume: 15 Pages: 508-508
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Application of a cell microarray chip system for accurate, highly sensitive, and rapid diagnosis for malaria in Uganda. (2016)
  • Author(s): Yatsushiro S, Yamamoto T, Yamamura S, Abe K, Obana E, Nogami T, Hayashi T, Sesei T, Oka H, Okello-Onen J, Odongo-Aginya EI, Alai MA, Olia A, Anywar D, Sakurai M, Palacpac NM, Mita T, Horii T, Baba Y and Kataoka M.
  • Journal Title: Scientific reports Volume: 6 Pages: 30136-30136
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Little polymorphism at the K13 propeller locus in worldwide Plasmodium falciparum populations prior to the introduction of artemisinin combination therapies. (2016)
  • Author(s): Mita T*, Culleton R, Takahashi N, Nakamura M, Tsukahara T, Hunja CW, Win ZZ, Htike WW, Marma AS, Dysoley L, Ndounga M, Dzodzomenyo M, Akhwale WS, Kobayashi J, Uemura H, Kaneko A, Hombhanje F, Ferreira MU, Bjorkman A, Endo H, Ohashi J.
  • Journal Title: Antimicrobial agents and chemotherapy Volume: 60 Pages: 3340-3347
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Plasmodium falciparum Kelch 13: A potential molecular marker for tackling artemisinin-resistant malaria parasites. (2016)
  • Author(s): Mita T*, Tachibana S, Hashimoto M, Hirai M.
  • Journal Title: Expert Rev Anti Infect Ther Volume: 14 Pages: 125-135
  • Peer Reviewed
• [Journal Article] Little polymorphism at the K13 propeller locus in worldwide Plasmodium falciparum populations prior to the introduction of artemisinin combination therapies. (2016)
  • Author(s): Mita, T, R Culleton, N Takahashi, M Nakamura, T Tsukahara, CW Hunja, ZZ Win, WW Htike, AS Marma and L Dysoley.
  • Journal Title: Antimicrobial agents and chemotherapy Volume: 1 Pages: 1-1
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Challenging malaria control: do our new genetic tools pave the way for malaria eradication? (2015)
  • Author(s): Hirai M, Mita T.
  • Journal Title: Juntendo Med J Volume: 61 Pages: 370-377
  • Peer Reviewed / Open Access
• [Journal Article] Current research topics in Tropical disease; toward the successful control and elimination; Introduction. (2015)
  • Author(s): Mita T*.
  • Journal Title: Juntendo Med J Volume: 61 Pages: 358-359
  • Peer Reviewed / Open Access
• [Journal Article] Patterns and dynamics of genetic diversity in Plasmodium falciparum: what past Human migrations tell us about malaria. (2015)
  • Author(s): Mita T*, Jombart, T.
  • Journal Title: Parasitol Int Volume: 64(3) Pages: 238-243
  • DOI: 10.1016/j.parint.2014.09.007
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Global distribution of polymorphisms associated with delayed Plasmodium falciparum parasite clearance following artemisinin treatment: genotyping of archive blood samples. (2015)
  • Author(s): Murai K, Culleton R, Hisaoka T, Endo H, Mita T*.
  • Journal Title: Parasitol Int Volume: 64(3) Pages: 267-273
  • DOI: 10.1016/j.parint.2014.11.002
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Plasmodium falciparum: Genetic diversity and complexity of infections in an isolated village in western Thailand (2015)
  • Author(s): K. Tanabe, G. Zollner, J. A. Vaughan, J. Sattabongkot, B. Khuntirat, H. Honma, T. Mita, T. Tsuboi and R. Coleman
  • Journal Title: Parasitology International Volume: pii: S1383-5769(13) Pages: 142-146
  • DOI: 10.1016/j.parint.2013.09.011
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] フィールドで何が起こっているのかをもっと知りたい-Comprehensiveな薬剤耐性研究. (2018)
  • Author(s): 美田敏宏
  • Organizer: 第87回日本寄生虫学会大会
• [Presentation] Altered gut microbiota composition in Plasmodium falciparum patients in Uganda. (2018)
  • Author(s): Taniguchi T, Miyauchi E, Olia A, Nagayasu E, Osbert K, Suzue K, Imai T, Shimokawa C, Onishi R, Odongo-Aginya E, Palacpac N, Maruyama H, Kimura E, Mita T, Ohno H, Horii T, Hisaeda H.
  • Organizer: 14th International congress of parastiology.
  • Int'l Joint Research
• [Presentation] Global issue of drug resistant malaria and population structure of malaria parasites for global malaria control. (2018)
  • Author(s): Mita T, Tachibana S, Sekihara M, Yamauchi M, Hombhanje F, Ohashi J.
  • Organizer: 14th International congress of parastiology.
  • Int'l Joint Research
• [Presentation] Detection of Artemisinin-resistant Plasmodium falciparum with High Survival Rates in Uganda. (2018)
  • Author(s): Mita T, Ikeda M, Kaneko M, Tachibana S, Balikagala B, Sakurai-Yatsushiro M, Yatsushiro S, Takahashi N, Yamauchi M, Sekihara M, Hashimoto M, Obwoya P, Auma M, Anywar D, Odongo-Aginya E, Hirai M, Ohashi J, Palacpac N, Kataoka M, Tsuboi T, Kimura E, Horii T.
  • Organizer: 14th International congress of parastiology.
  • Int'l Joint Research
• [Presentation] Detection of artemisinin-resistant Plasmodium falciparum isolates with ex-vivo ring-stage survival assay in Uganda. (2017)
  • Author(s): Mita T, Ikeda M, Kaneko M, Balikagala B, Tachibana S-I, Sakurai-Yatsushiro M, Yatsushiro S, Takahashi N, Hashimoto M, Katuro O, Olia A, Obwoya P, Auma M, Anywar D, Odongo-Aginya E, Hirai M, Palacpac N, Kataoka M, Tsuboi T, Kimura E,Horii T.
  • Organizer: Keystone Symposia Conference: Malaria: From Innovation to Eradication.
  • Place of Presentation: Speke Resort & Conference Centre, Kampala. Uganda
  • Year and Date: 2017-02-19
  • Int'l Joint Research
• [Presentation] 原虫学・寄生虫学が現代のトップサイエンスにもたらす貢献 マラリアミューテーター マラリア原虫薬剤耐性進化解明の新たな研究ツール. (2017)
  • Author(s): 平井誠, 池田美恵, 橘真一郎, 美田敏宏.
  • Organizer: 生命科学系学会合同年次大会.
• [Presentation] 人類を助ける進化学研究：進化し続ける薬剤耐性マラリアの克服. (2017)
  • Author(s): 美田敏宏
  • Organizer: 公益信託 進化学振興木村資生基金 “微生物と人類の共存”をゲノムの視点から考える.
  • Invited
• [Presentation] End malaria-マラリアのeliminationをめざして アルテミシニン耐性の評価・検出ツールは出そろったか? 流行地でのフィールド調査から見えてきた問題点. (2017)
  • Author(s): 美田敏宏
  • Organizer: 生命科学系学会合同年次大会.
• [Presentation] ウガンダにおけるin vivo薬剤耐性試験およびRSAを用いたアルテミシニン耐性疫学調査. (2017)
  • Author(s): 池田美恵, 橘真一郎, 山内祐人, Auma M, 関原誠, 森稔幸, 平井誠, Palacpac N, 木村英作, Aginya E, 堀井俊宏, 美田敏宏.
  • Organizer: 第86回日本寄生虫学会大会.
• [Presentation] ウガンダ国の熱帯熱マラリア患者において腸内細菌叢の変化が起こる. (2017)
  • Author(s): 谷口委代, 宮内栄治, OliaAlex, 長安英治, OsbertKaturo, 鈴江一友, 今井孝, 下川周子, 大西里咲, I. O-A, PalacpacNirianne, 丸山治彦, 木村英作, 美田敏宏, 大野博司, 堀井俊宏, 久枝一.
  • Organizer: グローバルヘルス合同大会プログラム・抄録集.
• [Presentation] ネズミマラリア原虫の有性生殖関連分子の機能解析. (2017)
  • Author(s): 前多晟壮, 森稔幸, 平井誠, 美田敏宏.
  • Organizer: 第86回日本寄生虫学会大会.
• [Presentation] マラリア原虫有性生殖の透視観察法の開発. (2017)
  • Author(s): 森稔幸, 平井誠, 美田敏宏
  • Organizer: 第86回日本寄生虫学会大会.
• [Presentation] ウガンダにおける熱帯熱マラリア原虫キニーネ感受性の実態. (2017)
  • Author(s): 山内祐人, 橘真一郎, 池田美恵, 関原誠, Balikagala B, 八代&櫻井美樹, 平井誠, Palacpac N, 木村英作, 堀井俊宏, 美田敏宏.
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] 線虫ミトコンドリアのプロテオーム解析 二次元電気泳動法とショットガン質量分析. (2017)
  • Author(s): 高宮信三郎, 數野彩子, 峯木礼子, 三浦芳樹, 美田敏宏.
  • Organizer: 生命科学系学会合同年次大会
• [Presentation] Ascaris suum 成虫およびCaenorhabditis elegans ミトコンドリアのプロテオーム解析: 二次元ゲル電気泳動法とショットガン質量分析法. (2017)
  • Author(s): 高宮信三郎, 数野彩子, 峯木礼子, 三浦芳樹, 美田敏宏.
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] 宿主内競争による熱帯熱マラリア原虫のクロロキン感受性の回復. (2017)
  • Author(s): 橘真一郎, 池田美恵, Balikagala B, 櫻井美樹, 八代聖基, 関原誠, 山内祐人, Auma MA, 平井誠, 森稔幸, Palacpac N, 片岡正俊, 木村英作, 堀井俊宏, 美田敏宏.
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] Epidemiology of antimalarial drug resistance in Papua New Guinea. (2017)
  • Author(s): 関原誠, 橘真一郎, 美田敏宏.
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] Prerequisite Emergence of Background Mutations For Kelch13-Related Artemisinin-Resistant Plasmodium Falciparum Isolates. (2017)
  • Author(s): Tachibana S-I, Mori T, Hirai M, Mita T,
  • Organizer: American Society of Tropical Medicine and Hygiene 66th Annual Meeting
  • Int'l Joint Research
• [Presentation] Development of See-Through Imaging Methods for Sexual Reproduction of Malaria Parasites. (2017)
  • Author(s): Mori T, Hirai M, Mita T,
  • Organizer: American Society of Tropical Medicine and Hygiene 66th Annual Meeting
  • Int'l Joint Research
• [Presentation] Detection of artemisinin-resistant Plasmodium falciparum isolates with ex-vivo ring-stage survival assay in Uganda. (2017)
  • Author(s): Mita T, Ikeda M, Kaneko M, Balikagala B, Tachibana S-I, Sakurai-Yatsushiro M, Yatsushiro S, Takahashi N, Hashimoto M, Katuro O, Olia A, Obwoya P, Auma M, Anywar D, Odongo-Aginya E, Hirai M, Palacpac N, Kataoka M, Tsuboi T, Kimura E, Horii T.
  • Organizer: Keystone Symposia Conference: Malaria
  • Int'l Joint Research
• [Presentation] A mutator Plasmodium berghei model to investigate antimalarial drug resistance. (2017)
  • Author(s): Mita T,
  • Organizer: American Society of Tropical Medicine and Hygiene 66th Annual Meeting
  • Int'l Joint Research / Invited
• [Presentation] Pb102, A Novel Gene Essential for Female Fertility or Ookinete Maturation of Murine Malaria Parasite, Plasmodium Berghei. (2017)
  • Author(s): Maeta A, Hirai M, Mori T, Mita T
  • Organizer: American Society of Tropical Medicine and Hygiene 66th Annual Meeting
  • Int'l Joint Research
• [Presentation] Application of a cell microarray chip system for accurate, highly sensitive, and rapid diagnosis for malaria. (2017)
  • Author(s): Kataoka M, Yatsushiro S, Yamamoto T, Oka H, Okello-Onen J, Odongo-Aginya E, Palacpac N, Mita T, Baba Y, Horii T.
  • Organizer: Keystone Symposia Conference: Malaria:
  • Int'l Joint Research
• [Presentation] Isolation of Piperaquine Resistant Rodent Malaria Parasite from Mutator Malaria. (2017)
  • Author(s): Hirai M, Ikeda M, Tachibana S-I, Mita T,
  • Organizer: American Society of Tropical Medicine and Hygiene 66th Annual Meeting
  • Int'l Joint Research
• [Presentation] マラリアの脅威とそれに立ち向かう新規技術の開発. (2016)
  • Author(s): 美田敏宏
  • Organizer: 第57回日本熱帯医学会総会市民講座「蚊がもたらす感染症から身を守る」
  • Place of Presentation: 一橋大学一橋講堂、東京.
  • Year and Date: 2016-11-06
  • Invited
• [Presentation] 新規ex-vivo薬剤耐性検出法を用いた高度感染地域におけるアルテミシニン耐性熱帯熱マラリア原虫の検出 (2016)
  • Author(s): 美田敏宏
  • Organizer: 第15回分子寄生虫マラリア研究フォーラム
  • Place of Presentation: 帯広畜産大学、帯広
  • Year and Date: 2016-08-22
• [Presentation] Detection of artemisinin-resistant Plasmodium falciparum isolates using novel ex-vivo assay in Uganda. (2016)
  • Author(s): Mita T.
  • Organizer: TICAD VI Pre-event; The 3rd International Symposium for the Promotion of Science and Technology Innovation Cooperation between Africa and Japan. -Life Innovation and Green Innovation.
  • Place of Presentation: JICA Ichigaya Building, Tokyo
  • Year and Date: 2016-07-13
  • Invited
• [Presentation] 細胞チップを応用した超高感度マラリア診断装置の開発 (2016)
  • Author(s): 美田敏宏
  • Organizer: 臨床研究情報センターセミナー. 先端医療振興財団
  • Place of Presentation: 臨床研究情報センター、神戸
  • Year and Date: 2016-05-24
  • Invited
• [Presentation] アフリカ大陸におけるアルテミシニン耐性マラリアの出現 (2016)
  • Author(s): 池田 美恵 、金子 恵、橘 真一郎、Mawagali Betty Balikagala、櫻井 美樹 、八代 聖基、 橋本 宗明 、Alex Olia 、Anywar Dennis 、Auma Mary 、Palacpac Nirianne M. Q. 、平井 誠 、 片岡 正俊 、木村 英作 、Aginya Emmanuel 、坪井 敬文 、堀井 俊宏 、美田 敏宏
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ
  • Year and Date: 2016-03-18
• [Presentation] ミューテーターマラリア原虫ライブラリーから薬剤耐性原虫の単離 (2016)
  • Author(s): 平井 誠、池田 美恵、橘 真一郎、美田 敏宏
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ
  • Year and Date: 2016-03-18
• [Presentation] 全世界の熱帯熱マラリア原虫株を用いたアルテミシニン耐性進化の集団遺伝学的解析 (2016)
  • Author(s): 橘 真一郎、平井 誠、美田 敏宏
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ
  • Year and Date: 2016-03-18
• [Presentation] ウガンダにおけるマラリア対策を見据えた包括的熱帯熱マラリア薬剤耐性研究 (2015)
  • Author(s): 美田敏宏
  • Organizer: 日本熱帯医学会総会シンポジウム
  • Place of Presentation: 大阪大学
  • Year and Date: 2015-12-04
  • Invited
• [Presentation] Comprehensive analysis of Artemether-Lumefantrine efficacy among children with uncomplicated malaria in Northern Uganda. (2015)
  • Author(s): Betty Balikagala1, Miki Sakurai2, Mie Ikeda3, Yatsushiro Shouki5, Nobuyuki Takahashi 2, Mary Auma6, Edward H. Ntege1, Daisuke Ito1, Eizo Takashima, Nirianne Marie Q Palacpac4, Joseph Okello Onen7, Masatoshi Kataoka 5, Kimura Eisaku4, Toshihiro Horii4, Toshihiro Mita3, Takafumi Tsuboi1
  • Organizer: 64th Annual Meeting American Society of Tropical Medicine and Hygiene
  • Place of Presentation: Philadelphia
  • Year and Date: 2015-10-25
• [Presentation] マラリアミューテーターを用いたピペラキン耐性原虫の迅速単離と耐性機構の解明 (2015)
  • Author(s): 池田美恵、平井誠、美田敏宏
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 東京、杏林大学 三鷹キャンパス
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] ウガンダにおけるex vivo薬剤感受性試験を用いた熱帯熱マラリア原虫のクロロキン感受性の回復 (2015)
  • Author(s): 櫻井美樹、Mawagali Betty Balikagala、矢代聖基、池田美恵、Ntege Edward、佐伯亜美、本間一、Palacpac Nirianne M.Q.、片岡正俊、坪井敬文、木村英作、遠藤弘良、堀井俊宏、美田敏宏
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 東京、杏林大学 三鷹キャンパス
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] Temporal patterns of molecular marker associated with artemisinin resistance before and after the implementation of artemisinin derivatives in Myanmar (2015)
  • Author(s): Win Zin Zayar、上村春樹、Wah Win Htike、Khin San San、北潔、美田敏宏
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 東京、杏林大学 三鷹キャンパス
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] アルテミシニン耐性熱帯熱マラリア原虫出現以前の大規模検体を用いた耐性候補遺伝子Ｋ１３-propellerのベースライン多型解析 (2015)
  • Author(s): 美田敏宏、高橋延之、遠藤弘良、大橋順
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 東京、杏林大学 三鷹キャンパス
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] ウガンダにおけるRing Stage Survival Assayを用いたアルテミシニン感受性低下熱帯熱マラリア原虫のスクリーニング (2015)
  • Author(s): 金子恵、池田美恵、Mawagali Betty Balikagala、櫻井美樹、矢代聖基、加納永将、佐伯亜美、Palacpac Nirianne M.Q.、片岡正俊、坪井敬文、木村英作、堀井俊宏、美田敏宏
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 東京、杏林大学 三鷹キャンパス
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] ミューテーターマラリア原虫を用いた突然変異蓄積パターンの解析 (2015)
  • Author(s): 本間一、平井誠、新倉保、美田敏宏、小林富美恵、堀井俊宏、遠藤弘良
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 東京、杏林大学 三鷹キャンパス
  • Year and Date: 2015-03-21 – 2015-03-22