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Verification of the insular adaptation of Protobothrops flavoviridis (Hon habu) distributed in the southwestern islands of Japan.

Research Project

Project/Area Number 26340095
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental and ecological symbiosis
Research InstitutionSojo University

Principal Investigator

CHIJIWA TAKAHITO  崇城大学, 生物生命学部, 教授 (30331060)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords生物多様性 / 適応
Outline of Final Research Achievements

The composition and the primary structures of the venom isozymes, mainly phospholipase A2s (PLA2), of Okinawa Protobothrops flavoviridis were compared among the snakes captured in different places. The places were Kunigami village and Itoman city, whose environment such as soil are independent each other. As a result, the composition and primary structures of the PLA2 isozymes were common as inherent to P. flavoviridis of Okinawa district, but also the area-specific subtypes of PLA2 isozymes were included. In this study, the structural analysis of the proteins and the genes demonstrates the plasticity of individuals, the diversity of small groups specific to the area, and the dynamics where such specimens converge to a large group phenotype.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (17 results)

All 2016 2015 2014

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (15 results) (of which Invited: 2 results)

  • [Journal Article] The taxonomic position and the unexpected divergence of the Habu viper, Protobothrops among Japanese subtropical islands.2016

    • Author(s)
      Shibata H, Chijiwa T, Hattori S, Terada S, Ohno M, Fukumaki Y
    • Journal Title

      Mol. Phylogenet. Evol. (Epub ahead of print)

      Volume: Epub ahead of print Pages: 91-100

    • DOI

      10.1016/j.ympev.2016.04.027

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Interisland variegation of venom [Lys49]phospholipase A2 isozyme genes in Protobothrops genus snakes in the southwestern islands of Japan2015

    • Author(s)
      Yamaguchi Y, Chijiwa T, Yamamura T, Ikeda N, Yatsui T, Hayama S, Hattori S, Oda-Ueda N, Ohno M
    • Journal Title

      Toxicon

      Volume: 107 Pages: 210-216

    • DOI

      10.1016/j.toxicon.2015.08.024

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] クサリヘビ科ヘビの分泌型ホスホリパーゼA2(PLA2)をコードする遺伝子クラスター領域の解析2016

    • Author(s)
      稲丸賢人、千々岩崇仁、他6名
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、神奈川県、横浜市
    • Year and Date
      2016-12-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] 毒蛇ハブ(Protobothrops flavoviridis)の全ゲノム配列決定から明らかになった毒液関連遺伝子群の多重化および加速進化と染色体構造との関係2016

    • Author(s)
      柴田弘紀、千々岩崇仁、他20名
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、神奈川県、横浜市
    • Year and Date
      2016-12-01
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ハブ血清に含まれる新規ハブ毒活性中和因子の単離・特徴づけ及び、ハブ毒阻害血清タンパク質の混合画分の調整2016

    • Author(s)
      平江敦
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、神奈川県、横浜市
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 日本固有の毒蛇ハブ(Protobothrops flavoviridis )の全ゲノム配列決定と遺伝子モデルの作製2016

    • Author(s)
      柴田弘紀、千々岩崇仁、他18名
    • Organizer
      第63回トキシンシンポジウム
    • Place of Presentation
      天童温泉、山形県、天童市
    • Year and Date
      2016-07-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒメハブ分泌型ホスリパーゼA2(PLA2)をコードする遺伝子クラスター領域の解読と解析2016

    • Author(s)
      稲丸賢人、千々岩崇仁、他7名
    • Organizer
      第63回トキシンシンポジウム
    • Place of Presentation
      天童温泉、山形県、天童市
    • Year and Date
      2016-07-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 毒蛇ハブ幼蛇と成蛇の毒成分の比較解析2016

    • Author(s)
      中村仁美、千々岩崇仁、他4名
    • Organizer
      第63回トキシンシンポジウム
    • Place of Presentation
      天童温泉、山形県、天童市
    • Year and Date
      2016-07-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 日本固有の毒蛇ハブ(Protobothrops flavoviridis )の全ゲノム配列決定と遺伝子モデルの作製2015

    • Author(s)
      柴田弘紀
    • Organizer
      BMB2105
    • Place of Presentation
      兵庫県、神戸市中央区、神戸ポートアイランド
    • Year and Date
      2015-12-03
    • Related Report
      2015 Research-status Report
  • [Presentation] ハブ毒腺における幼蛇特異的ホスホリパーゼA2アイソザイム遺伝子の発現2015

    • Author(s)
      中村仁美
    • Organizer
      BMB2105
    • Place of Presentation
      兵庫県、神戸市中央区、神戸ポートアイランド
    • Year and Date
      2015-12-02
    • Related Report
      2015 Research-status Report
  • [Presentation] ハブ毒由来不活性型セリンプロテアーゼ様タンパク質の構造と機能2015

    • Author(s)
      清水安奈
    • Organizer
      BMB2105
    • Place of Presentation
      兵庫県、神戸市中央区、神戸ポートアイランド
    • Year and Date
      2015-12-02
    • Related Report
      2015 Research-status Report
  • [Presentation] 日本南西諸島クサリヘビ科ヘビの毒[Lys49]ホスホリパーゼA2アイソザイム遺伝子の島嶼多様性2015

    • Author(s)
      千々岩崇仁
    • Organizer
      BMB2015
    • Place of Presentation
      兵庫県、神戸市中央区、神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] クサリヘビ科ヘビの毒ホスホリパーゼA2遺伝子の形成と起源2015

    • Author(s)
      山口和晃
    • Organizer
      BMB2015
    • Place of Presentation
      兵庫県、神戸市中央区、神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Whole genome sequencing of a Japanese endemic pit viper, habu, Protobothrops flavoviridis and whole genome annotation.2015

    • Author(s)
      柴田弘紀
    • Organizer
      日本遺伝学会第87回大会
    • Place of Presentation
      宮城県、仙台市青葉区、東北大学
    • Year and Date
      2015-09-26
    • Related Report
      2015 Research-status Report
  • [Presentation] 毒腺で発現する転写因子ESE-3はハブ毒PLA2遺伝子のプロモーターを活性化する2015

    • Author(s)
      中村仁美
    • Organizer
      第62回トキシンシンポジウム
    • Place of Presentation
      三重県、志摩市、エクシード合歓の郷
    • Year and Date
      2015-07-10
    • Related Report
      2015 Research-status Report
  • [Presentation] クサリヘビ科ヘビの筋壊死活性ホスホリパーゼA2 ([Lys49]PLA2) 遺伝子の分子進化2014

    • Author(s)
      千々岩崇仁
    • Organizer
      第37回 日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、神奈川県、横浜市
    • Year and Date
      2014-11-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 日本固有の毒蛇ハブ(Protobothrops flavoviridis)の全ゲノム配列決定の現状と展望2014

    • Author(s)
      柴田弘紀
    • Organizer
      第51回 沖縄生物学会
    • Place of Presentation
      琉球大学、沖縄県、中頭郡
    • Year and Date
      2014-05-25
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-12-27  

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