Elucidation of the mechanism by which virus inhibits the function of human anti-virus enzymes and molecular basis of drug discovery
| Project/Area Number |
26440026
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
Nagata Takashi 京都大学, エネルギー理工学研究所, 准教授 (10415250)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAORI Akifumi 京都大学, 医学部附属病院, 教授 (20324626)
KATAHIRA Masato 京都大学, エネルギー理工学研究所, 教授 (60265717)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | Vif / APOBEC / アプタマー / NMR |
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| Outline of Final Research Achievements |
We have developed a stable-isotope labeling method of the Vif complex, which contains five proteins. Our method is capable of labeling either Vif or CBFβ, while the rest of the components are unlabeled. Obtained labeled Vif complex can be used to identify the binding sites of the anti-HIV proteins (A3F, A3G, etc.) and anti-Vif compounds on the Vif complex. We also obtained the RNA aptamers that bind to the Vif complex with high affinity. On the other hand, we investigated the influence of the DNA sequence/length and pH on deaminase activity, as well as the roles of the amino acid residues around the catalytic center of APOBEC3F. We newly found that the Vif complex inhibits the catalytic activity of the C-terminal domain of A3G. Our efforts will provide the tools for anti-Vif drug development.
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Report
(5 results)
Research Products
(142 results)
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[Journal Article] Molecular Landscape of the Ribosome Pre-initiation Complex during mRNA Scanning: Structural Role for eIF3c and Its Control by eIF52017
Author(s)
Obayashi E, Luna RE, Nagata T, Martin-Marcos P, Hiraishi H, Singh CR, Erzberger JP, Zhang F, Arthanari H, Morris J, Pellarin R, Moore C, Harmon I, Papadopoulos E, Yoshida H, Nasr ML, Unzai S, Thompson B, Aube E, Hustak S, Stengel F, Dagraca E, Ananbandam A, Gao P, Urano T, Hinnebusch AG, Wagner G, Asano K.
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Journal Title
Cell Report
Volume: 18
Issue: 11
Pages: 2651-2663
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] The binding specificity of translocated in liposarcoma/fused in sarcoma with lncRNA transcribed from the promoter region of cyclin D12016
Author(s)
Yoneda, R., Suzuki, S., Mashima, T., Kondo, K., Nagata, T., Katahira, M. and Kurokawa, R.
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Journal Title
Cell Biosci.
Volume: 6(4)
Issue: 1
Pages: 1-12
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] リグノセルロースの糖－リグニン複合体とその酵素反応の解析2017
Author(s)
西村 裕志,左近 静香, Jenny Arnling Baath, Amanda Ristinmaa, Johanna Nilsson, Gunnar Westman, Lisbeth Olsson, 永田 一真, 永田 崇, 片平 正人, 渡辺 隆司
Organizer
第67回日本木材学会大会
Place of Presentation
九州大学（福岡市）
Year and Date
2017-03-17
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[Presentation] Non-coding RNA/DNA recognition by TLS/FUS that causes repression of cyclin D1 transcription and telomere elongation2016
Author(s)
Kondo, K., Mashima, T., Oyoshi, T., Yoneda, R., Kurokawa, R., Nagata, T. and Katahira, M.
Organizer
The 43rd International Symposium on Nucleic Acids Chemistry
Place of Presentation
熊本大学（熊本市）
Year and Date
2016-09-27
Related Report
Int'l Joint Research
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[Presentation] Accurate and molecular-size-tolerant NMR quantitation of diverse compounds, and real-time monitoring of enzymatic reaction2016
Author(s)
Okamura, H., Kamba, K., Nishimura, H., Kigawa, T., Watanabe, T., Nagata, T., Katahira, M.
Organizer
The XXVIIth International Conference on Magnetic Resonance in Biological Systems
Place of Presentation
国立京都国際会館（京都市）
Year and Date
2016-08-21
Related Report
Int'l Joint Research
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[Presentation] Analysis of molecular interaction of peptides with lignin for lignocellulosic biorefinery2016
Author(s)
Watanabe, T., Yamaguchi, A., Oshiro, S., Suetomi, T., Nishimura, H., Nagata, T., Mashima, T., Katahira, M., Isozaki, K., Takaya, H. and Nakamura, M.
Organizer
4th Symposium on Biotechnology applied to Lignocelluloses
Place of Presentation
CSIC downtown Campus (Madrid)
Year and Date
2016-06-19
Related Report
Int'l Joint Research
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[Presentation] NMR study of the recognition of non-coding RNA and DNA by TLS/FUS, a key regulator of cyclin D12015
Author(s)
Kondo, K., Mashima, T., Oyoshi, T., Kurokawa, R., Nagata, T. and Katahira, M.
Organizer
The 42nd International Symposium on Nucleic Acids Chemistry
Place of Presentation
イーグレ姫路 (姫路市)
Year and Date
2015-09-23
Related Report
Int'l Joint Research
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[Presentation] New strategy for high-throughput NMR analysis of biomolecules using the NMR database BMRB and tools for automated NMR analysis, MagRO, FLYA and CYANA2015
Author(s)
N. Kobayashi, M. Yokochi, T. Iwata, BR. Sahoo, T. Nagata, JL. Markley, EL. Ulrich, E. Schmidt, P. Güntert, C. Kojima and T. Fujiwara
Organizer
International Society of Magnetic Resonance
Place of Presentation
China Executive Leadership Academy (Shanghai)
Year and Date
2015-08-16
Related Report
Int'l Joint Research
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[Presentation] Real-time NMR monitoring of enzymatic reaction of anti-HIV protein, structure of anti-prion RNA aptamer and wood biomass analysis2014
Author(s)
Kanba, K., Mashima, T., Okamura, H., Furukawa, A., Sugase, K., Nishimura, H., Watanabe, T., Nagata, T., and Katahira, M.
Organizer
The 5th Japan-Taiwan NMR symposium
Place of Presentation
北海道大学（札幌市）
Year and Date
2014-09-29 – 2014-09-30
Related Report
Invited
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[Presentation] PROPERTIES OF RNA APTAMER BINDING TO AML1 RUNT DOMAIN2014
Author(s)
R. Amano, Y. Nomura, T. Nagata, N. Kobayashi, Y. Mori, K. Takada, J. Fukunaga, Y. Tanaka, M. Katahira, Y. Nakamura, T. Kozu, T. Sakamoto
Organizer
XXI ROUND TABLE ON NUCLEOSIDES, NUCLEOTIDES AND NUCLEIC ACIDS
Place of Presentation
Conference Centre of Poznan University of Technology (Poland)
Year and Date
2014-08-24 – 2014-08-28
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