Elucidation of the mechanism by which virus inhibits the function of human anti-virus enzymes and molecular basis of drug discovery

• Project/Area Number: 26440026
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyoto University
• Principal Investigator: Nagata Takashi 京都大学, エネルギー理工学研究所, 准教授 (10415250)
• Co-Investigator(Renkei-kenkyūsha): TAKAORI Akifumi 京都大学, 医学部附属病院, 教授 (20324626) ; KATAHIRA Masato 京都大学, エネルギー理工学研究所, 教授 (60265717)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: Vif / APOBEC / アプタマー / NMR

Outline of Final Research Achievements

We have developed a stable-isotope labeling method of the Vif complex, which contains five proteins. Our method is capable of labeling either Vif or CBFβ, while the rest of the components are unlabeled. Obtained labeled Vif complex can be used to identify the binding sites of the anti-HIV proteins (A3F, A3G, etc.) and anti-Vif compounds on the Vif complex. We also obtained the RNA aptamers that bind to the Vif complex with high affinity. On the other hand, we investigated the influence of the DNA sequence/length and pH on deaminase activity, as well as the roles of the amino acid residues around the catalytic center of APOBEC3F. We newly found that the Vif complex inhibits the catalytic activity of the C-terminal domain of A3G. Our efforts will provide the tools for anti-Vif drug development.

Research Products

• [Int'l Joint Research] Goethe University Frankfurt am Main(Germany)
• [Int'l Joint Research] National Institute of Health/Kansas State University/UCSF(米国)
• [Int'l Joint Research] ETH Zurich(Switzerland)
• [Int'l Joint Research] Universite Laval(Canada)
• [Journal Article] Mechanism of protein-RNA recognition: analysis based on the statistical mechanics of hydration (2018)
  • Author(s): Hayashi Tomohiko, Matsuda Tomoaki, Nagata Takashi, Katahira Masato, Kinoshita Masahiro
  • Journal Title: Phys Chem Chem Phys Volume: 20 Issue: 14 Pages: 9167-9180
  • DOI: 10.1039/c8cp00155c
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of the glutathione S-transferases that belong to the GSTFuA class in Ceriporiopsis subvermispora : Implications in intracellular detoxification and metabolism of wood-derived compounds (2018)
  • Author(s): Osman Wan Hasnidah Wan、Lin Meng-I、Kondo Keiko、Nagata Takashi、Katahira Masato
  • Journal Title: Int J Biol Macromol Volume: 113 Pages: 1158-1166
  • DOI: 10.1016/j.ijbiomac.2018.03.029
  • Peer Reviewed / Open Access
• [Journal Article] Characterisation of an aptamer against the Runt domain of AML1 (RUNX1) by NMR and mutational analyses (2018)
  • Author(s): Takada Kenta、Amano Ryo、Nomura Yusuke、Tanaka Yoichiro、Sugiyama Shigeru、Nagata Takashi、Katahira Masato、Nakamura Yoshikazu、Kozu Tomoko、Sakamoto Taiichi
  • Journal Title: FEBS Open Bio Volume: 8 Issue: 2 Pages: 264-270
  • DOI: 10.1002/2211-5463.12368
  • NAID: 120006457124
  • Peer Reviewed / Open Access
• [Journal Article] Plastic roles of phenylalanine and tyrosine residues of TLS/FUS in complex formation with the G-quadruplexes of telomeric DNA and TERRA (2018)
  • Author(s): Kondo Keiko、Mashima Tsukasa、Oyoshi Takanori、Yagi Ryota、Kurokawa Riki、Kobayashi Naohiro、Nagata Takashi、Katahira Masato
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 2864-2864
  • DOI: 10.1038/s41598-018-21142-1
  • Peer Reviewed / Open Access
• [Journal Article] High yield production of fungal manganese peroxidases by E. coli through soluble expression, and examination of the activities (2018)
  • Author(s): Lin Meng-I、Nagata Takashi、Katahira Masato
  • Journal Title: Protein Expr Purif Volume: 145 Pages: 45-52
  • DOI: 10.1016/j.pep.2017.12.012
  • Peer Reviewed / Open Access
• [Journal Article] The first successful observation of in-cell NMR signals of DNA and RNA in living human cells (2018)
  • Author(s): Yamaoki Yudai、Kiyoishi Ayaka、Miyake Masayuki、Kano Fumi、Murata Masayuki、Nagata Takashi、Katahira Masato
  • Journal Title: Phys Chem Chem Phys Volume: 20 Issue: 5 Pages: 2982-2985
  • DOI: 10.1039/c7cp05188c
  • NAID: 130007557810
  • Peer Reviewed / Open Access
• [Journal Article] The C-terminal cytidine deaminase domain of APOBEC3G itself undergoes intersegmental transfer for a target search, as revealed by real-time NMR monitoring (2018)
  • Author(s): Kamba Keisuke、Nagata Takashi、Katahira Masato
  • Journal Title: Phys Chem Chem Phys Volume: 20 Issue: 5 Pages: 2976-2981
  • DOI: 10.1039/c7cp05171a
  • Peer Reviewed / Open Access
• [Journal Article] Influence of the DNA sequence/length and pH on deaminase activity, as well as the roles of the amino acid residues around the catalytic center of APOBEC3F (2018)
  • Author(s): Wan Li、Nagata Takashi、Katahira Masato
  • Journal Title: Phys Chem Chem Phys Volume: 20 Issue: 5 Pages: 3109-3117
  • DOI: 10.1039/c7cp04477a
  • Peer Reviewed / Open Access
• [Journal Article] Conjugation of two RNA aptamers improves binding affinity to AML1 Runt domain (2017)
  • Author(s): Nomura Yusuke、Yamazaki Kaori、Amano Ryo、Takada Kenta、Nagata Takashi、Kobayashi Naohiro、Tanaka Yoichiro、Fukunaga Junichi、Katahira Masato、Kozu Tomoko、Nakamura Yoshikazu、Haishima Yuji、Torigoe Hidetaka、Sakamoto Taiichi
  • Journal Title: J Biochem Volume: 162 Issue: 6 Pages: 431-436
  • DOI: 10.1093/jb/mvx049
  • NAID: 40021421327
  • Peer Reviewed / Open Access
• [Journal Article] Observation by Real-Time NMR and Interpretation of Length- and Location-Dependent Deamination Activity of APOBEC3B (2017)
  • Author(s): Wan Li、Nagata Takashi、Morishita Ryo、Takaori-Kondo Akifumi、Katahira Masato
  • Journal Title: ACS Chem Biol Volume: 12 Issue: 11 Pages: 2704-2708
  • DOI: 10.1021/acschembio.7b00662
  • Peer Reviewed / Open Access
• [Journal Article] Backbone and side chain assignments of the second RNA-binding domain of Musashi-1 in its free form and in complex with 5-mer RNA (2017)
  • Author(s): Iwaoka Ryo、Nagata Takashi、Tsuda Kengo、Imai Takao、Okano Hideyuki、Kobayashi Naohiro、Katahira Masato
  • Journal Title: Biomol NMR Assign Volume: 11 Issue: 2 Pages: 265-268
  • DOI: 10.1007/s12104-017-9760-9
  • Peer Reviewed / Open Access
• [Journal Article] Structural Insight into the Recognition of r(UAG) by Musashi-1 RBD2, and Construction of a Model of Musashi-1 RBD1-2 Bound to the Minimum Target RNA (2017)
  • Author(s): Iwaoka Ryo、Nagata Takashi、Tsuda Kengo、Imai Takao、Okano Hideyuki、Kobayashi Naohiro、Katahira Masato
  • Journal Title: Molecules Volume: 22 Issue: 7 Pages: 1207-1207
  • DOI: 10.3390/molecules22071207
  • NAID: 120006492019
  • Peer Reviewed / Open Access
• [Journal Article] Development of an RNA aptamer that acquires binding capacity against HIV-1 Tat protein via G-quadruplex formation in response to potassium ions (2017)
  • Author(s): Yamaoki, Y., Nagata, T., Mashima, T., Katahira, M.
  • Journal Title: Chemical communications Volume: 53 Issue: 52 Pages: 7056-7059
  • DOI: 10.1039/c7cc03312e
  • Peer Reviewed / Open Access
• [Journal Article] Molecular Landscape of the Ribosome Pre-initiation Complex during mRNA Scanning: Structural Role for eIF3c and Its Control by eIF5 (2017)
  • Author(s): Obayashi E, Luna RE, Nagata T, Martin-Marcos P, Hiraishi H, Singh CR, Erzberger JP, Zhang F, Arthanari H, Morris J, Pellarin R, Moore C, Harmon I, Papadopoulos E, Yoshida H, Nasr ML, Unzai S, Thompson B, Aube E, Hustak S, Stengel F, Dagraca E, Ananbandam A, Gao P, Urano T, Hinnebusch AG, Wagner G, Asano K.
  • Journal Title: Cell Report Volume: 18 Issue: 11 Pages: 2651-2663
  • DOI: 10.1016/j.celrep.2017.02.052
  • NAID: 120006358788
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structure, dynamics and interaction of p54nrb/NonO RRM1 with 5' Splice Site RNA sequence. (2016)
  • Author(s): Duvignaud JB, Bedard M, Nagata T, Muto Y, Yokoyama S, Gagne; SM, Vincent M
  • Journal Title: Biochemistry Volume: 0 Issue: 18 Pages: 0-0
  • DOI: 10.1021/acs.biochem.5b01240
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The binding specificity of translocated in liposarcoma/fused in sarcoma with lncRNA transcribed from the promoter region of cyclin D1 (2016)
  • Author(s): Yoneda, R., Suzuki, S., Mashima, T., Kondo, K., Nagata, T., Katahira, M. and Kurokawa, R.
  • Journal Title: Cell Biosci. Volume: 6(4) Issue: 1 Pages: 1-12
  • DOI: 10.1186/s13578-016-0068-8
  • NAID: 120005763383
  • Peer Reviewed / Open Access
• [Journal Article] Accurate and molecular-size-tolerant NMR quantitation of diverse components in solution (2016)
  • Author(s): Okamura, H., Nishimura, H., Nagata, T., Kigawa, T., Watanabe, T. and Katahira M.
  • Journal Title: Sci. Rep. Volume: 6(21742) Issue: 1 Pages: 1-12
  • DOI: 10.1038/srep21742
  • NAID: 120005705947
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of the deamination coupled with sliding along DNA of anti-HIV factor APOBEC3G on the basis of the pH-dependence of deamination revealed by real-time NMR monitoring (2016)
  • Author(s): Kamba K, Nagata T, Katahira M
  • Journal Title: Front. Microbiol. Volume: 0 Pages: 0-0
  • DOI: 10.3389/fmicb.2016.00587
  • NAID: 120005947002
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] K+-responsive off-to-on switching of hammerhead ribozyme through dual G-quadruplex formation requiring no heating and cooling treatment (2015)
  • Author(s): Yamaoki Y, Nagata T, Mashima T, Katahira M
  • Journal Title: Biochemical and biophysical research communications Volume: 468(1-2) Issue: 1-2 Pages: 27-31
  • DOI: 10.1016/j.bbrc.2015.10.173
  • NAID: 120005712951
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Boosting of activity enhancement of K+-responsive quadruplex hammerhead ribozyme (2015)
  • Author(s): Yudai Yamaoki, Tsukasa Mashima, Takashi Nagata and Masato Katahira
  • Journal Title: Chemical Communications Volume: 51 Issue: 27 Pages: 5898-5901
  • DOI: 10.1039/c5cc00961h
  • Peer Reviewed / Open Access
• [Journal Article] Catalytic analysis of APOBEC3G involving real-time NMR spectroscopy reveals nucleic acid determinants for deamination (2015)
  • Author(s): K. Kamba, T. Nagata, M. Katahira
  • Journal Title: PLOS ONE Volume: 10 Issue: 4 Pages: e0124142-e0124142
  • DOI: 10.1371/journal.pone.0124142
  • NAID: 120005668069
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structural basis for inhibition of the MDM2:p53 interaction by an optimized MDM2-binding peptide selected with mRNA display (2014)
  • Author(s): T. Nagata, K. Shirakawa, N. Kobayashi, H. Shiheido, N. Tabata, Y. Sakuma-Yonemura, K. Horisawa, M. Katahira, N. Doi, H. Yanagawa
  • Journal Title: PLOS ONE Volume: 9 Issue: 10 Pages: e109163-e109163
  • DOI: 10.1371/journal.pone.0109163
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] セルラーゼ糖質結合モジュールとリグニン間相互作用部位のNMRによる解析 (2018)
  • Author(s): 徳永有希、大城理志、永田崇、近藤敬子、西村裕志、片平正人、渡辺隆司
  • Organizer: 第68回日本木材学会
• [Presentation] リグノセルロースの糖－リグニン複合体とその酵素反応の解析 (2017)
  • Author(s): 西村 裕志,左近 静香, Jenny Arnling Baath, Amanda Ristinmaa, Johanna Nilsson, Gunnar Westman, Lisbeth Olsson, 永田 一真, 永田 崇, 片平 正人, 渡辺 隆司
  • Organizer: 第67回日本木材学会大会
  • Place of Presentation: 九州大学（福岡市）
  • Year and Date: 2017-03-17
• [Presentation] 安定同位体標識法を用いたセルラーゼ糖質結合モジュールとリグニン間相互作用のNMRによる解析 (2017)
  • Author(s): 徳永有希、大城理志、永田崇、近藤敬子、西村裕志、片平正人、渡辺隆司
  • Organizer: 第67回日本木材学会大会
  • Place of Presentation: 九州大学（福岡市）
  • Year and Date: 2017-03-17
• [Presentation] Characterization of Glutathione-Dependent Enzymes from White Rot Fungi (2017)
  • Author(s): Wan Hasnidah Wan Osman, Meng-I Lin, Keiko Kondo, Takashi Nagata, Masato Katahira
  • Organizer: 日本農芸化学会2017年度京都大会
  • Place of Presentation: ウェスティン都ホテル京都（京都市）
  • Year and Date: 2017-03-17
• [Presentation] Heterologous expression of the ligninolytic enzymes from wood rotting fungi and analysis of peroxidase activity, lignin model degradation, and homologous modeling (2017)
  • Author(s): Meng-I Lin, Keiko Kondo, Takashi Nagata, Masato Katahira
  • Organizer: 日本農芸化学会2017年度京都大会
  • Place of Presentation: ウェスティン都ホテル京都（京都市）
  • Year and Date: 2017-03-17
• [Presentation] Characterization of the deamination activity of APOBEC3B by real-time NMR, which is distinct from that of APOBEC3G (2017)
  • Author(s): Li Wan, Takashi Nagata, Ryo Morishita, Akifumi Takaori-Kondo, Masato Katahira
  • Organizer: 7th Asia-Pacific NMR Symposium
  • Place of Presentation: Indian Institute of Science (Bangalore)
  • Year and Date: 2017-02-16
  • Int'l Joint Research
• [Presentation] Sliding and intersegmental transfer along DNA enhance the enzymatic activity of APOBEC3G as revealed by real-time NMR monitoring methods (2017)
  • Author(s): Keisuke Kamba, Takashi Nagata, Masato Katahira
  • Organizer: 7th Asia-Pacific NMR Symposium
  • Place of Presentation: Indian Institute of Science (Bangalore)
  • Year and Date: 2017-02-16
  • Int'l Joint Research
• [Presentation] リアルタイムNMRモニタリング法による酵素反応の定量解析及びヒト抗ウイルス因子ＡＰＯＢＥＣ３Ｇの脱アミノ化機構の解明 (2017)
  • Author(s): 神庭圭佑、永田崇、片平正人
  • Organizer: 定量生物学の会年会
  • Place of Presentation: 岡崎コンファレンスセンター（岡崎）
  • Year and Date: 2017-01-08
• [Presentation] The effect of different target nucleic acids on the structural change of TLS (2017)
  • Author(s): Nesreen Hamad, Tsukasa Mashima, Hiroki Watanabe, Takayuki Uchihashi, Riki Kurokawa, Takashi Nagata, Masato Katahira
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] Modeling of the tandem RNA-binding domains of a neural and oncogenic protein, Musashi1, engaging the minimum target RNA (2017)
  • Author(s): Ryo Iwaoka, Takashi Nagata, Kengo Tsuda, Takao Imai, Hideyuki Okano, Naohiro Kobayashi, Masato Katahira
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] Studies on lytic polysaccharide monooxygenase (LPMO) from the selective white rot fungus, Ceriporiposis subvermispora (2017)
  • Author(s): Yuu Iseki, Satoshi Oshiro, Takashi Nagata, Keiko Kondo, Hiroshi Nishimura, Masato Katahira, Takashi Watanabe
  • Organizer: Humanosphere Science School 2017
  • Int'l Joint Research
• [Presentation] Analysis of Adsorption Mechanism between Carbohydrate Binding Module of Cellulase and Lignin by NMR (2017)
  • Author(s): Yuki Tokunaga, Satoshi Oshiro, Takashi Nagata, Keiko Kondo, Hiroshi Nishimura, Masato Katahira, Takashi Watanabe
  • Organizer: Humanosphere Science School 2017
  • Int'l Joint Research
• [Presentation] Analysis of Lignin-Carbohydrate Complexes in Woody Biomass by Using NMR (2017)
  • Author(s): Shizuka Sakon, Hiroshi Nishimura, Misato Yamada, Yukari Ohta, Keiko Kondo, Yudai Yamaoki, Takashi Nagata, Masato Katahira, Takashi Watanabe
  • Organizer: Humanosphere Science School 2017
  • Int'l Joint Research
• [Presentation] Characterization of the cytidine deamination properties of human APOBEC3B by real-time NMR, which are quite different from those of APOBEC3G (2017)
  • Author(s): Li Wan, Takashi Nagata, Ryo Morishita, Akifumi Takaori-Kondo, Masato Katahira
  • Organizer: The 44th International Symposium on Nucleic Acids Chemistry, The 1st Annual Meeting of Japan Society of Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] 翻訳開始前複合体において形成される翻訳開始因子間の結合ネットワークの役割 (2017)
  • Author(s): 永田崇、吉田尚志、雲財悟、浦野健、浅野桂、尾林栄治
  • Organizer: 第56回NMR討論会
• [Presentation] In-cell NMR法によるヒト細胞内DNAおよびRNAの観測 (2017)
  • Author(s): 山置 佑大, 清石 彩華, 三宅雅之, 加納 ふみ, 村田 昌之, 永田 崇, 片平 正人
  • Organizer: 第56回NMR討論
• [Presentation] RNA結合タンパク質Musashi-1のRBD2:r(GUAGU)複合体溶液構造決定と標的RNA選択メカニズムの解明 (2017)
  • Author(s): 岩岡諒，永田崇，津田健吾，今井貴雄，岡野栄之，小林直宏，片平正人
  • Organizer: 第56回NMR討論
• [Presentation] 深層学習により強化されたNMR立体構造解析 (2017)
  • Author(s): 小林直宏、永田崇、服部良一、新家粧子、Julia M. Wurz、Peter Guntert、児嶋長次郎、藤原敏道
  • Organizer: 第56回NMR討論
• [Presentation] Analysis of peroxidase activity, lignin model degradation of heterologous expressed soluble fungal ligninolytic enzymes (2017)
  • Author(s): Meng-I Lin, Keiko Kondo, Takashi Nagata, Masato Katahira
  • Organizer: 2017 Taiwan-Japan Biomedical Symposium on Magnetic Resonance
  • Int'l Joint Research
• [Presentation] Sphingombium sp. YG1由来guaiacylglycerol-β-guaiacyl ether - Cα位酸化酵素の酵素学的解析と利用 (2017)
  • Author(s): 前田亜鈴悠、宋晶、西真郎、黒澤佳奈子、市川淳子、 塩谷美夏、山置佑大、永田崇、片平正人、山田美沙登、渡辺隆司、大田ゆかり
  • Organizer: 第62回リグニン討論会
• [Presentation] フラグメント分子軌道法を活用したリグニンβ-O-4還元開裂酵素の機能改変 (2017)
  • Author(s): 嶋根康弘、黒澤佳奈子、市川淳子、前田亜鈴悠、布浦拓郎、山置佑大、永田崇、片平正人、高谷光、吉田亮太、中村正治、渡辺隆司、大田ゆかり
  • Organizer: 第62回リグニン討論会
• [Presentation] Kinetic studies of the two fungal glutathione-dependent enzymes and their potential roles in the intracellular detoxification and wood-derived compound metabolism (2017)
  • Author(s): Wan Hasnidah Wan Osman, Meng-I Lin, Keiko Kondo, Takashi Nagata, Masato Katahira
  • Organizer: 第62回リグニン討論会
• [Presentation] 18-crown-6を用いるヒドロニウム溶媒和イオン液体の基礎物性 (2017)
  • Author(s): 北田敦、竹岡駿、近都康平、深見一弘、才村正幸、永田崇、片平正人、邑瀬邦明
  • Organizer: 2017年電気化学会秋季大会
• [Presentation] Development of the artificial multi-domain enzymes immobilized on the curdlan sheet at low cost. (2017)
  • Author(s): Masataka Horiuchi, Takashi Nagata, Masato Katahira, Yoshihiro Kobashigawa, Yasuhiko Suzuki, Masanori Ochiai
  • Organizer: The 8th International Symposium of Advanced Energy Science
  • Int'l Joint Research
• [Presentation] Structure-function relationship of Epstein-Barr Virus EBNA3C NLS and transport receptor (2017)
  • Author(s): Go Matsuda, Takashi Nagata, Masato Katahira, Ken-ichi Imadome
  • Organizer: The 8th International Symposium of Advanced Energy Science
  • Int'l Joint Research
• [Presentation] Development of RNA binding peptides based on tertiary structural information (2017)
  • Author(s): Natsuki Watanabe, Tomoaki Kinase, Takashi Nagata, Masato Katahira, Kazuo Harada, and Taiichi Sakamoto
  • Organizer: The 8th International Symposium of Advanced Energy Science
  • Int'l Joint Research
• [Presentation] テロメア長短縮をもたらすTLS/FUS蛋白質とテロメアDNAおよびTERRAのグアニン四重鎖との複合体に関するNMR解析 (2017)
  • Author(s): 近藤 敬子、 真嶋 司、大吉 崇文、黒川 理樹、小林直宏、永田 崇、片平 正人
  • Organizer: 第55回生物物理学会
• [Presentation] Influences of length and concentration of the DNA substrate, as well as the location of the target sequence, on deamination by APOBEC3F (2017)
  • Author(s): Li Wan, Takashi Nagata, Masato Katahira
  • Organizer: 第55回生物物理学会
• [Presentation] An anti-prion aptamer inhibits the formation of prion protein-amyloid β oligomer complex that is related to Alzheimer's disease (2017)
  • Author(s): Mamiko IIDA, Tsukasa MASHIMA, Yudai YAMAOKI, Takashi NAGATA, Masato KATAHIRA
  • Organizer: 第55回生物物理学会
• [Presentation] NMR Analysis of Interaction Mechanism between Carbohydrate Binding Module of Cellulase and Lignin (2017)
  • Author(s): Yuki Tokunaga, Satoshi Oshiro, Keiko Kondo, Takashi Nagata, Hiroshi Nishimura, Masato Katahira, Takashi Watanabe
  • Organizer: The 2nd Asia Research Node Symposium on Humanosphere Science
  • Int'l Joint Research
• [Presentation] TLS/FUSの非コードRNAの認識機構の構造学的研究 (2017)
  • Author(s): 真嶋司, 小澤 駿介, Nesreen Hamad, 米田竜馬, 黒川 理樹, 永田崇, 片平 正人
  • Organizer: 第19回日本RNA学会年会
• [Presentation] Conformational change detection of translocated in liposarcoma, TLS, upon binding to various RNAs (2017)
  • Author(s): Nesreen Hamad, Tsukasa Mashima,Yudai Yamaoki, Keiko Kondo, Takashi Nagata, Masato Katahira
  • Organizer: 第19回日本RNA学会年会
• [Presentation] NMR法によるセルラーゼ糖質結合モジュール-リグニン間相互作用の解明 (2017)
  • Author(s): 徳永有希、大城理志、永田崇、近藤敬子、西村裕志、片平正人、渡辺隆司
  • Organizer: 第３１回セルラーゼ研究会
• [Presentation] Highly automated NMR signal assignments using peak-noise recognition assisted by Deep Neural Networks (2017)
  • Author(s): Kobayashi, N., Nagata, T., Kojima, C., and Fujiwara, T.
  • Organizer: Gordon Research Conference. Computational Aspects-Biomol NMR
  • Int'l Joint Research
• [Presentation] TLS/FUSのRNA認識機構と結合に伴うコンフォーメーション変化の研究 (2017)
  • Author(s): 真嶋 司, 小澤 駿介, Nesreen Hamad, 米田 竜馬, 黒川 理樹, 永田 崇, 片平 正人
  • Organizer: 第17回日本蛋白質科学会年会
• [Presentation] High yield production, activity analysis, and homologous modeling of soluble fungal peroxidases expressed in E. coli (2017)
  • Author(s): Meng-I Lin, Keiko Kondo, Takashi Nagata, Masato Katahira
  • Organizer: 第17回日本蛋白質科学会年会
• [Presentation] APOBEC3Gのストランド間移動の実時間NMR法検出 (2017)
  • Author(s): 神庭圭佑，永田崇，片平正人
  • Organizer: 第17回日本蛋白質科学会年会
• [Presentation] HIV-1 RRE由来のRNA断片と人工ペプチドの相互作用の解析 (2017)
  • Author(s): 渡邊 なつき，木名瀬智章，永田崇，片平正人，原田和雄，坂本泰一
  • Organizer: 平成29年度日本生化学会関東支部例会
• [Presentation] Development of K+-responsive RNA aptamer and ribozyme, and in-cell NMR of nucleic acids in human cells (2017)
  • Author(s): Y. Yamaoki, A. Kiyoishi, T. Mashima, F. Kano, M. Murata, T. Nagata, M. Katahira
  • Organizer: International Symposium for NMR of Nucleic Acids
  • Place of Presentation: Gyeongsang National Univ. (Korea)
  • Int'l Joint Research
• [Presentation] NMR study for the recognition of non-coding RNA and DNA by TLS/FUS, a key regulator of the transcriptional repression by cyclin D1 and telomere (2017)
  • Author(s): K. Kondo, T. Mashima, T. Oyoshi, R. Yoneda, R. Kurokawa, T. Nagata, M. Katahira
  • Organizer: International Symposium for NMR of Nucleic Acids
  • Place of Presentation: Gyeongsang National Univ. (Korea)
  • Int'l Joint Research
• [Presentation] 非コードRNAと結合してcyclin D1転写を抑制するTLS/FUSのRNA認識機構の構造学的基盤 (2016)
  • Author(s): 真嶋司, 小澤 駿介, 近藤 敬子, Nesreen Hamad, 米田竜馬, 黒川 理樹, 永田崇, 片平 正人
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2016-11-30
• [Presentation] プリオンタンパク質とプリオンタンパク質を標的とするRNA分子のAβ線維化への影響 (2016)
  • Author(s): 飯田 真美子, 真嶋 司, 山置 佑大, 永田 崇, 片平正人
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場（つくば市）
  • Year and Date: 2016-11-25
• [Presentation] スライディングとストランド間移動を用いたヒト抗ウイルス因子APOBEC3Gの高効率なDNA配列探索：実時間NMRによる新知見 (2016)
  • Author(s): 神庭 圭佑、永田 崇、片平 正人
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場（つくば市
  • Year and Date: 2016-11-25
• [Presentation] Characterization of the deamination activity of APOBEC3B by real-time NMR, which is distinct from that of APOBEC3G (2016)
  • Author(s): Li Wan, Takashi Nagata, Ryo Morishita, Akifumi Takaori-Kondo, Masato Katahira
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場（つくば市
  • Year and Date: 2016-11-25
• [Presentation] 深層学習に支援されたNMRシグナルの自動解析 (2016)
  • Author(s): 小林直宏、服部良一、永田崇、児嶋長次郎、藤原敏道
  • Organizer: 第55回NMR討論会
  • Place of Presentation: 広島国際会議（広島市）
  • Year and Date: 2016-11-16
• [Presentation] Cyclin D1遺伝子転写制御やテロメア長制御に関わるTLS/FUSタンパク質と非コード核酸間の相互作用のNMR法による解析 (2016)
  • Author(s): 近藤 敬子, 真嶋 司, 大吉 崇文, 米田 竜馬, 黒川 理樹, 永田 崇, 片平 正人
  • Organizer: 第55回NMR討論会
  • Place of Presentation: 広島国際会議場（広島市）
  • Year and Date: 2016-11-16
• [Presentation] 木質バイオマスにおける超分子構造とステレオ選択的な酵素反応及び核酸のin-cell NMR (2016)
  • Author(s): 山置佑大，永田一真、清石彩華、西村裕志、加納ふみ、村田昌之、大田ゆかり、渡辺隆司、永田崇、片平正人
  • Organizer: 第55回NMR討論会
  • Place of Presentation: 広島国際会議場（広島市
  • Year and Date: 2016-11-16
• [Presentation] Heterologous expression and structure-activity relationship analysis of the oxidative enzymes from wood rotting fungi that are involved in lignocellulose degradation (2016)
  • Author(s): Meng-I Lin, Keiko Kondo, Takashi Nagata, Masato Katahira
  • Organizer: 第61回リグニン討論会
  • Place of Presentation: 京都大学（宇治市）
  • Year and Date: 2016-10-27
• [Presentation] リグノセルロースバイオリファイナリーのためのリグニン・ペプチド間相互作用解析 (2016)
  • Author(s): 山口亜佐子、末富高志、大城理志、徳永有希、西村裕志、永田崇、真嶋司、 片平正人、磯﨑勝弘、高谷光、中村正治、渡辺隆司
  • Organizer: 第61回リグニン討論会
  • Place of Presentation: 京都大学（宇治市）
  • Year and Date: 2016-10-27
• [Presentation] 海洋性細菌に由来するリグニンβ-O-4還元開裂酵素GST4の解析 (2016)
  • Author(s): 嶋根康弘、前田亜鈴悠、塩谷美夏、黒澤佳奈子、市川淳子、 布浦拓郎、山置佑大、永田崇、片平正人、高谷光、 吉田亮太、中村正治、渡辺隆司、大田ゆかり
  • Organizer: 第61回リグニン討論会
  • Place of Presentation: 京都大学（宇治市）
  • Year and Date: 2016-10-27
• [Presentation] Investigation of the interactions between eukaryotic initiation factors implicated during ribosomal scanning and start codon recognition (2016)
  • Author(s): Takashi Nagata
  • Organizer: The 42nd Naito Conference on “In the Vanguard of Structural Biology: Revolutionizing Life Sciences
  • Place of Presentation: シャトレーゼガトーキングダム（札幌市）
  • Year and Date: 2016-10-04
  • Int'l Joint Research
• [Presentation] Non-coding RNA/DNA recognition by TLS/FUS that causes repression of cyclin D1 transcription and telomere elongation (2016)
  • Author(s): Kondo, K., Mashima, T., Oyoshi, T., Yoneda, R., Kurokawa, R., Nagata, T. and Katahira, M.
  • Organizer: The 43rd International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 熊本大学（熊本市）
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] カリウムイオン応答性Tat結合RNAアプタマーおよびハンマーヘッドリボザイムの創製およびin-cell NMR法による細胞内核酸の観測 (2016)
  • Author(s): 山置 佑大, 清石 彩華, 真嶋 司, 加納 ふみ, 村田 昌之, 永田 崇, 片平 正人
  • Organizer: 第10回バイオ関連化学シンポジウム、第31回生体機能関連化学シンポジウム、第19回バイオテクノロジー部会シンポジウム
  • Place of Presentation: 石川県立音楽堂（金沢市）
  • Year and Date: 2016-09-07
• [Presentation] Development of artificial RNA and artificial peptide to control gene expression by using NMR (2016)
  • Author(s): Yumi Takeda, Takashi Nagata, Ryo Amano, Yusuke Nomura, Kazuo Harada, Colin A. Smith, Masato Katahira, Taiichi Sakamoto
  • Organizer: The 7th International Symposium of Advanced Energy Science
  • Place of Presentation: 京都大学（宇治市）
  • Year and Date: 2016-09-05
  • Int'l Joint Research
• [Presentation] Low-cost degradation of azo dyes using the GRP-tagged azoreductase combined with NADPH regeneration enzymes immobilized on the curdlan sheet (2016)
  • Author(s): Masataka Horiuchi, Yasuhiko Suzuki, Masanori Ochiai, Takashi Nagata, Masato Katahira
  • Organizer: The 7th International Symposium of Advanced Energy Science
  • Place of Presentation: 京都大学（宇治市）
  • Year and Date: 2016-09-05
  • Int'l Joint Research
• [Presentation] Structural study of RNA aptamer and peptide that potently perturb the function of intrinsically disordered proteins related to disease (2016)
  • Author(s): Takashi Nagata
  • Organizer: The 5th International Symposium on Drug Discovery and Design by NMR
  • Place of Presentation: 理化学研究所横浜キャンパス（横浜市）
  • Year and Date: 2016-08-29
  • Int'l Joint Research / Invited
• [Presentation] Accurate and molecular-size-tolerant NMR quantitation of diverse compounds, and real-time monitoring of enzymatic reaction (2016)
  • Author(s): Okamura, H., Kamba, K., Nishimura, H., Kigawa, T., Watanabe, T., Nagata, T., Katahira, M.
  • Organizer: The XXVIIth International Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] K+-responsive Activation of Ribozyme System via Structural Transition from Duplex to Dual-quadruplex Requiring no Heating and Cooling Treatment (2016)
  • Author(s): Yudai Yamaoki, Takashi Nagata, Tsukasa Mashima, Masato Katahira
  • Organizer: The XXVIIth International Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] NMR analysis of the recognition of non-coding RNA and DNA by TLS/FUS which causes transcriptional repression of cyclin D1 and telomere shortening (2016)
  • Author(s): Tsukasa Mashima, Keiko Kondo, Takanori Oyoshi, Ryoma Yoneda, Riki Kurokawa, Takashi Nagata, Masato Katahira
  • Organizer: The RNA Society of Japan 18th Annual Meeting AND The 21st Annual Meeting of the RNA Society
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] Binding properties of high-affinity RNA aptamers against AML1 Runt domain (2016)
  • Author(s): Ryo Amano, Kenta Takada, Yoichiro Tanaka, Takashi Nagata, Masato Katahira, Yoshikazu Nakamura, Tomoko Kozu, Taiichi Sakamoto
  • Organizer: The RNA Society of Japan 18th Annual Meeting AND The 21st Annual Meeting of the RNA Society
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] K+-responsive activation of dual-quadruplex forming ribozyme system requiring no heating and cooling treatment (2016)
  • Author(s): Yudai Yamaoki, Tsukasa Mashima, Takashi Nagata, Masato Katahira
  • Organizer: The RNA Society of Japan 18th Annual Meeting AND The 21st Annual Meeting of the RNA Society
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] Analysis of molecular interaction of peptides with lignin for lignocellulosic biorefinery (2016)
  • Author(s): Watanabe, T., Yamaguchi, A., Oshiro, S., Suetomi, T., Nishimura, H., Nagata, T., Mashima, T., Katahira, M., Isozaki, K., Takaya, H. and Nakamura, M.
  • Organizer: 4th Symposium on Biotechnology applied to Lignocelluloses
  • Place of Presentation: CSIC downtown Campus (Madrid)
  • Year and Date: 2016-06-19
  • Int'l Joint Research
• [Presentation] プリオン蛋白質とそれを標的とする RNA 分子の A β線維化への影響 (2016)
  • Author(s): 飯田 真美子, 真嶋 司, 山置 佑大, 永田 崇, 片平正人
  • Organizer: 第16回日本蛋白質科学会年会
  • Place of Presentation: 福岡国際会議場（福岡市）
  • Year and Date: 2016-06-07
• [Presentation] Cyclin D1転写抑制を担う TLS/FUS タンパク質と非コード RNA の相互作用の NMR 法による解析 (2016)
  • Author(s): 真嶋司, 小澤 駿介, 近藤 敬子, 永田崇, 黒川 理樹, 片平 正人
  • Organizer: 第16回日本蛋白質科学会年会
  • Place of Presentation: 福岡国際会議場（福岡市）
  • Year and Date: 2016-06-07
• [Presentation] Binding properties of RNA aptamer against AML1 Runt domain (2016)
  • Author(s): R. Amano, K. Takada, T. Nagata, Y. Nomura, J. Fukunaga, Y. Tanaka, M. Katahira, Y. Nakamura, T. Kozu and T. Sakamoto
  • Organizer: Aptamer 2016
  • Place of Presentation: St Hilda's College (Oxford)
  • Year and Date: 2016-04-04
  • Int'l Joint Research
• [Presentation] Tandem GGA repeat G-quadruplex as an anti-prion protein aptamer and a K+-responsive structural/functional switching device (2016)
  • Author(s): T. Nagata, T. Mashima, Y. Yamaoki, F. Nishikawa, Y.O. Kamatari, S. Nishikawa, K. Kuwata and M. Katahira
  • Organizer: Aptamer 2016
  • Place of Presentation: St Hilda's College (Oxford)
  • Year and Date: 2016-04-04
  • Int'l Joint Research
• [Presentation] 転写因子AML1のDNA結合部位を標的とする高親和性RNAアプタマーの開発と解析 (2016)
  • Author(s): 天野亮，高田健多，田中陽一郎， 永田崇， 片平正人， 中村義一， 神津知子，坂本泰一
  • Organizer: 第5回日本生物物理学会関東支部会
  • Place of Presentation: 桐生地域地場産業振興センター (桐生市)
  • Year and Date: 2016-03-10
• [Presentation] 疾患に関わる天然変性蛋白質の昨日を阻害するRNAアプタマーと高親和性ペプチドのNMRによる構造機能相関解析 (2016)
  • Author(s): 永田 崇
  • Organizer: 第4回ネオバイオ分子研究会
  • Place of Presentation: 大阪府立大学 (大阪市)
  • Year and Date: 2016-01-29
  • Invited
• [Presentation] Binding properties of RNA aptamer that binds to the transcription factor AML1 Runt domain (2016)
  • Author(s): R. Amano, K. Takada, Y. Tanaka, T. Nagata, M. Katahira, Y. Nakamura, T. Kozu and T. Sakamoto
  • Organizer: The 8th Takeda Science Foundation Symposium on PharmaSciences
  • Place of Presentation: 武田薬品研修所 (大阪)
  • Year and Date: 2016-01-21
  • Int'l Joint Research
• [Presentation] NMR study of RNA aptamer that binds to the transcription factor AML1 Runt domain (2016)
  • Author(s): T. Sakamoto, R. Amano, Y. Nomura, T. Nagata, M. Katahira, J. Fukunaga, Y. Tanaka, Y. Nakamura and T. Kozu
  • Organizer: The 8th Takeda Science Foundation Symposium on PharmaSciences
  • Place of Presentation: 武田薬品研修所 (大阪)
  • Year and Date: 2016-01-21
  • Int'l Joint Research
• [Presentation] Invention of K+-responsive Tat-binding RNA aptamer and hammerhead ribozyme, and in-cell NMR of nucleic acids (2016)
  • Author(s): Yudai Yamaoki, Ayaka Kiyoishi, Tsukasa Mashima, Fumi Kano, Masayuki Murata, Takashi Nagata, Masato Katahira
  • Organizer: The 43rd International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 熊本大学（熊本市）
  • Int'l Joint Research
• [Presentation] 転写因子AML1 Runt domainを標的とした高親和性RNAアプタマーの特徴 (2015)
  • Author(s): 高田健多，天野亮，田中陽一郎，永田崇，片平正人，中村義一，神津知子，坂本泰一
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド (神戸市)
  • Year and Date: 2015-12-01
• [Presentation] Analysis of the biocharacteristics of APOBEC3F by real-time NMR (2015)
  • Author(s): L. Wan，K. Kamba，T. Nagata，M. Katahira
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド (神戸市)
  • Year and Date: 2015-12-01
• [Presentation] Heterologous expression and struature-activity relationship analysis of the oxidative enzymes involved in lignosellulose degradatioin from wood rotting fungi (2015)
  • Author(s): M. Lin，T. Nagata，B. Mikami，M. Katahira
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド (神戸市)
  • Year and Date: 2015-12-01
• [Presentation] NMRによる実時間酵素活性アッセイにより抗ウィルス因子APOBEC3Gの脱アミノ化機構を明らかにする (2015)
  • Author(s): 神庭圭佑，永田崇，片平正人
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド (神戸市)
  • Year and Date: 2015-12-01
• [Presentation] AML1 (RUNX1)タンパク質のRuntドメインとRNAアプタマーの相互作用のNMR解析 (2015)
  • Author(s): 天野亮，高田健多，田中陽一郎，永田崇，片平正人，野村祐介，福永淳一，中村義一，神津知子， 坂本泰一
  • Organizer: 日本核酸医薬学会第1回年会
  • Place of Presentation: 京都テルサ (京都市)
  • Year and Date: 2015-11-30
• [Presentation] リアルタイムNMR法の新たな展開ー抗HIVタンパク質APOBECG3Gの認識ヌクレチド、DNA上のスライディング及びエピジェネクスとの関連に関する新知見ー (2015)
  • Author(s): 神庭圭佑，永田崇，片平正人
  • Organizer: 第54回NMR討論会
  • Place of Presentation: 千葉工業大学 (千葉市)
  • Year and Date: 2015-11-06
• [Presentation] 転写因子AML1 Runt domainに結合する高親和性RNAアプタマーのNMR解析 (2015)
  • Author(s): 高田健太，天野亮，田中陽一郎，永田崇，片平正人，中村義一，神津知子，坂本泰一
  • Organizer: 第54回NMR討論会
  • Place of Presentation: 千葉工業大学 (千葉市)
  • Year and Date: 2015-11-06
• [Presentation] 木質バイオマスの特性を決めるリグニンー多糖結合様式の決定 (2015)
  • Author(s): 神谷明宏，西村裕志，永田一真，永田崇，渡辺隆司，片平正人
  • Organizer: 第54回NMR討論会
  • Place of Presentation: 千葉工業大学 (千葉市)
  • Year and Date: 2015-11-06
• [Presentation] 海洋性Novosphingubium属細菌に由来するリグニンモデル2量体β-O-4結合開裂酵素群の酵素学的解析 (2015)
  • Author(s): 大田ゆかり、黒澤佳奈子、永田崇、片平正人、西村裕志、渡辺隆司、長谷川良一、秦田勇二
  • Organizer: 第60回リグニン討論会
  • Place of Presentation: 筑波大学 (筑波市)
  • Year and Date: 2015-11-05
• [Presentation] NMR study of the recognition of non-coding RNA and DNA by TLS/FUS, a key regulator of cyclin D1 (2015)
  • Author(s): Kondo, K., Mashima, T., Oyoshi, T., Kurokawa, R., Nagata, T. and Katahira, M.
  • Organizer: The 42nd International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: イーグレ姫路 (姫路市)
  • Year and Date: 2015-09-23
  • Int'l Joint Research
• [Presentation] Nucleic acid determinants of deamination by human anti-HIV factor, APOBEC3G, as revealed by real-time NMR method (2015)
  • Author(s): Kamba, K., Nagata, T., and Katahira, M.
  • Organizer: The 42nd International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: イーグレ姫路 (姫路市)
  • Year and Date: 2015-09-23
  • Int'l Joint Research
• [Presentation] 効率よくウイルスDNAに変異を導入するAPOBEC3Gの脱アミノ化機構 (2015)
  • Author(s): 神庭 圭佑、永田 崇、片平 正人
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学 (金沢市)
  • Year and Date: 2015-09-13
• [Presentation] 異常型プリオン蛋白質の産生を抑制する四重鎖核酸の構造解析 (2015)
  • Author(s): 真嶋司，西川富美子，鎌足雄司，永田崇，西川諭，桑田一夫，片平正人
  • Organizer: 第９回バイオ関連化学シンポジウム
  • Place of Presentation: 熊本大学 (熊本市)
  • Year and Date: 2015-09-10
• [Presentation] カリウムイオンを認識して活性がスイッテングするTat結合RNAアプタマーおよびハンマーヘッドリボザイムの創製 (2015)
  • Author(s): 山置佑大，真嶋司，永田崇，片平正人
  • Organizer: 第９回バイオ関連化学シンポジウム
  • Place of Presentation: 熊本大学 (熊本市)
  • Year and Date: 2015-09-10
• [Presentation] Structural and biochemical studies on eIF1 with eIF3c and eIF5; rearrangement of subunit interaction for accurate recognition of AUC start codon (2015)
  • Author(s): Obayashi, E., Urano, T., Asano, K., Katahira, M., and Nagata, T.
  • Organizer: The 6th International Symposium of Advanced Energy Science～Towards the Realization of Zero-Emission Energy～
  • Place of Presentation: 京都大学 (宇治市)
  • Year and Date: 2015-09-01
  • Int'l Joint Research
• [Presentation] NMR analyses of RNA-peptide complexes for the development of biomolecules which regulate gene expression (2015)
  • Author(s): R. Amano, Y. Nomura, T. Nagata, N. Kobayashi, Y. Mori, J. Fukunaga, Y. Tanaka, M. Katahira, Y. Nakamura, T. Kozu and T. Sakamoto
  • Organizer: The 6th International Symposium of Advanced Energy Science～Towards the Realization of Zero-Emission Energy～
  • Place of Presentation: 京都大学 (宇治市)
  • Year and Date: 2015-09-01
  • Int'l Joint Research
• [Presentation] New strategy for high-throughput NMR analysis of biomolecules using the NMR database BMRB and tools for automated NMR analysis, MagRO, FLYA and CYANA (2015)
  • Author(s): N. Kobayashi, M. Yokochi, T. Iwata, BR. Sahoo, T. Nagata, JL. Markley, EL. Ulrich, E. Schmidt, P. Güntert, C. Kojima and T. Fujiwara
  • Organizer: International Society of Magnetic Resonance
  • Place of Presentation: China Executive Leadership Academy (Shanghai)
  • Year and Date: 2015-08-16
  • Int'l Joint Research
• [Presentation] Real-time NMR methods reveals deamination mechanism by human antiviral factor APOBEC3G (2015)
  • Author(s): K. Kamba, T. Nagata, M. Katahira
  • Organizer: 6th Asia-Pacific NMR Symposium
  • Place of Presentation: Hong Kong University of Science and Technology (Hong Kong)
  • Year and Date: 2015-08-13
  • Int'l Joint Research
• [Presentation] 転写抑制やテロメア短縮を引き起こすTLS/FUSと長鎖非コードRNAおよびDNA間結合の構造基盤 (2015)
  • Author(s): 近藤 敬子、 真嶋 司、大吉 崇文、黒川 理樹、永田 崇、片平 正人
  • Organizer: RNA meeting 2015
  • Place of Presentation: ホテルライフォート札幌 (札幌市)
  • Year and Date: 2015-07-15
• [Presentation] AML1タンパク質のDNA結合ドメインに対するRNAアプタマーの特性 (2015)
  • Author(s): 高田 健多，天野 亮，田中 陽一郎，永田 崇，片平 正人，中村 義一，神津 知子，坂本泰一
  • Organizer: RNA meeting 2015
  • Place of Presentation: ホテルライフォート札幌 (札幌市)
  • Year and Date: 2015-07-15
• [Presentation] AML1 タンパク質の Runt ドメインに結合する RNA アプタマーの取得と解析 (2015)
  • Author(s): 高田健多，天野亮，田中陽一郎，永田崇，片平正人，中村義一，神津知子， 坂本泰一
  • Organizer: 平成27年度日本生化学会関東支部例会
  • Place of Presentation: 新潟日報メディアシップ (新潟市)
  • Year and Date: 2015-06-20
• [Presentation] タンパク質の構造、相互作用、酵素反応のNMRによる解析 (2015)
  • Author(s): 永田 崇
  • Organizer: 第５６回 日本生化学会 中国-四国支部例会
  • Place of Presentation: 島根大学大学会館（松江市）
  • Year and Date: 2015-05-29 – 2015-05-30
  • Invited
• [Presentation] タンパク質の構造、相互作用、酵素反応のNMRによる解析 (2015)
  • Author(s): 永田 崇
  • Organizer: 第56回日本生化学会 中国-四国支部例会
  • Place of Presentation: 島根大学大学会館 (松江市)
  • Year and Date: 2015-05-29
  • Invited
• [Presentation] CCND1転写抑制とテロメア短縮をもたらすTLS/FUSによる非コードRNAとDNA認識機構のNMR法による解明 (2014)
  • Author(s): 近藤敬子，真嶋司，山置佑大，大吉崇文，黒川理樹，永田崇，片平正人
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 神経系幹細胞の未分化状態維持と発癌に関わるMusashi1タンパク質RBD2の構造機能解析 (2014)
  • Author(s): 岩岡諒，小林直宏，津田健吾，今井貴雄，岡野栄之，永田崇，片平正人
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 実時間NMR法による抗HIV因子APOBEC3Gのスライディングにカップルした脱アミノ化機構の解析 (2014)
  • Author(s): 神庭圭佑，永田崇，片平正人
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] AML1 Runt domainとRNAアプタマーの相互作用のNMR解析 (2014)
  • Author(s): 高田健多，天野亮，永田崇，片平正人，野村祐介，田中陽一郎，中村義一，神津知子，坂本泰一
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Deamination mechanism of an anti-HIV APOBEC3G coupled with sliding on single-stranded DNA as analyzed by real-time NMR monitoring method (2014)
  • Author(s): K. Kanba, A. Furukawa, K. Sugase, T. Nagata, M. Katahira
  • Organizer: The 41st International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 北九州国際会議場（北九州市）
  • Year and Date: 2014-11-05 – 2014-11-07
• [Presentation] Creation of novel Tat-binding aptamer and ribozyme whose activities switch on in response to K+ via quadruplex formation (2014)
  • Author(s): Y. Yamaoki, T. Mashima, T. Nagata, M. Katahira
  • Organizer: The 41st International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 北九州国際会議場（北九州市）
  • Year and Date: 2014-11-05 – 2014-11-07
• [Presentation] Characterization of RNA aptamer targeting the DNA binding domain of AML1 protein (2014)
  • Author(s): K. Takada, R. Amano, Y. Tanaka, T. Nagata, M. Katahira, Y. Nakamura, T. Kozu, T. Sakamoto
  • Organizer: The 41st International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 北九州国際会議場（北九州市）
  • Year and Date: 2014-11-05 – 2014-11-07
• [Presentation] 神経系幹細胞の未分化状態維持と発癌に関わるMusashi1タンパク質の構造基盤 (2014)
  • Author(s): 岩岡諒，小林直宏，津田健吾，今井貴雄，岡野栄之，永田崇，片平正人
  • Organizer: 第53回NMR討論会
  • Place of Presentation: 大阪大学コンベンションセンター（吹田市）
  • Year and Date: 2014-11-04 – 2014-11-06
• [Presentation] MagRO-NMRView、FLYAによる高度に自動化されたＮＭＲデータ解析 (2014)
  • Author(s): 小林直宏，Bikash Ranjan Sahoo，永田崇，服部良一，Elena Schmidt，Peter Güntert，児嶋長次郎，藤原敏道
  • Organizer: 第53回NMR討論会
  • Place of Presentation: 大阪大学コンベンションセンター（吹田市）
  • Year and Date: 2014-11-04 – 2014-11-06
• [Presentation] NMR study of the Interaction between MDM2 and a Peptide Selected by mRNA Display (2014)
  • Author(s): T. Nagata, K. Shirakawa, N. Kobayashi, H. Shiheido, N. Tabata, Y. Sakuma-Yonemura, K. Horisawa, M. Katahira, N. Doi, H. Yanagawa
  • Organizer: Biophysical Society Thematic Meeting Disordered Motifs and Domains in Cell Control
  • Place of Presentation: Davenport Hotel Dublin (Ireland)
  • Year and Date: 2014-10-11 – 2014-10-15
• [Presentation] NMR analysis of the interaction between an artificial RNA and a transcription factor (2014)
  • Author(s): K. Takada, R. Amano, T. Nagata, M. Katahira, Y. Nomura, Y. Tanaka, Y. Nakamura, T. Kozu, T. Sakamoto
  • Organizer: The 5th International Symposium of Advanced Energy Science -Contribution to Zero-Emission Energy-
  • Place of Presentation: 京都大学（宇治市）
  • Year and Date: 2014-09-30 – 2014-10-02
• [Presentation] Real-time NMR monitoring of enzymatic reaction of anti-HIV protein, structure of anti-prion RNA aptamer and wood biomass analysis (2014)
  • Author(s): Kanba, K., Mashima, T., Okamura, H., Furukawa, A., Sugase, K., Nishimura, H., Watanabe, T., Nagata, T., and Katahira, M.
  • Organizer: The 5th Japan-Taiwan NMR symposium
  • Place of Presentation: 北海道大学（札幌市）
  • Year and Date: 2014-09-29 – 2014-09-30
  • Invited
• [Presentation] Development of Tat-binding aptamer and ribozyme which switch their activities in response to K+ (2014)
  • Author(s): Y. Yamaoki, T. Mashima, T. Nagata, M. Katahira
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター（札幌市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Screening for novel aptamer against prion protein and its structural study (2014)
  • Author(s): T. Mashima, F. Nishikawa, Y.O. Kamatari, T. Nagata, S. Nishikawa, K. Kuwata, M. Katahira
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター（札幌市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Substrate Recognition and Sliding Properities of an Anti-HIV Factor APOBEC3G analyzed by Real-time NMR Monitoring Strategy (2014)
  • Author(s): K. Kamba, T. Nagata, M. Katahira
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター（札幌市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Functional mechanism of an anti-HIV protein, APOBEC3G, as revealed by real-time NMR monitoring of its enzymatic reaction (2014)
  • Author(s): K. Kanba, A. Furukawa, K. Sugase, R. Morishita, T. Nagata, M. Katahira
  • Organizer: XXVIth International Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: Hyatt Regency - Dallas(USA)
  • Year and Date: 2014-08-24 – 2014-08-29
• [Presentation] PROPERTIES OF RNA APTAMER BINDING TO AML1 RUNT DOMAIN (2014)
  • Author(s): R. Amano, Y. Nomura, T. Nagata, N. Kobayashi, Y. Mori, K. Takada, J. Fukunaga, Y. Tanaka, M. Katahira, Y. Nakamura, T. Kozu, T. Sakamoto
  • Organizer: XXI ROUND TABLE ON NUCLEOSIDES, NUCLEOTIDES AND NUCLEIC ACIDS
  • Place of Presentation: Conference Centre of Poznan University of Technology (Poland)
  • Year and Date: 2014-08-24 – 2014-08-28
• [Presentation] カリウムイオンを感知して自らの活性をスイッチングするTat捕捉アプタマーおよびリボザイムの創製 (2014)
  • Author(s): 山置佑大，真嶋司，永田崇，片平正人
  • Organizer: 第16回日本RNA学会年会
  • Place of Presentation: ウインクあいち（名古屋市）
  • Year and Date: 2014-07-23 – 2014-07-25
• [Presentation] Structural and functional analysis of Musashi, a neural and oncogenic RNA-binding protein (2014)
  • Author(s): R. Iwaoka, T. Nagata, T. Imai, H, Okano, M. Katahira
  • Organizer: 第16回日本RNA学会年会
  • Place of Presentation: ウインクあいち（名古屋市）
  • Year and Date: 2014-07-23 – 2014-07-25
• [Presentation] The structural and energetic basis for the high-potency inhibition of MDM2-p53 interaction by MDM2-binding peptide obtained with IVV method (2014)
  • Author(s): T. Nagata, K. Shirakawa, B.R. Sahoo, H. Shiheido, K. Horisawa, M. Katahira, N. Kobayashi, N. Doi, H. Yanagawa
  • Organizer: 第14回日本蛋白質科学会年会
  • Place of Presentation: ワークピア横浜（横浜市）
  • Year and Date: 2014-06-25 – 2014-06-27
• [Presentation] CCND1転写抑制とテロメア短縮に関与する天然変性蛋白質TLS/FUSによる非コードRNAとDNAの認識機構のNMR法による解明 (2014)
  • Author(s): 近藤敬子，真嶋司，大吉崇文，黒川理樹，永田崇，片平正人
  • Organizer: 第14回日本蛋白質科学会年会
  • Place of Presentation: ワークピア横浜（横浜市）
  • Year and Date: 2014-06-25 – 2014-06-27
• [Presentation] AML1タンパク質のRuntドメインに結合するRNAアプタマーの取得と解析 (2014)
  • Author(s): 高田健多，天野亮，永田崇，片平正人，中村義一，神津知子，坂本泰一
  • Organizer: 日本生化学会関東支部定例会
  • Place of Presentation: 茨城大学（水戸市）
  • Year and Date: 2014-06-14