Non-invasive monitoring and biomass estimation of salmonid fish using environmental DNA
| Project/Area Number |
26640136
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservation of biological resources
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| Research Institution | Hokkaido University |
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Principal Investigator |
ARAKI Hitoshi 北海道大学, 農学研究院, 教授 (20707129)
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| Co-Investigator(Kenkyū-buntansha) |
高原 輝彦 島根大学, 生物資源科学部, 助教 (10536048)
源 利文 神戸大学, 人間発達環境学研究科, 特命助教 (50450656)
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| Co-Investigator(Renkei-kenkyūsha) |
KONDO Michio 龍谷大学, 理工学部, 教授 (30388160)
DOI Hideyuki 兵庫県立大学, 大学院シミュレーション学研究科, 准教授 (80608505)
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| Research Collaborator |
SATO Shunpei 国立研究開発法人水産研究・教育機構, 北海道区水産研究所, 主任研究員 (70425461)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 環境DNA / サケ科魚類 / モニタリング / サケ / 定量 / 生物量 / 環境DNA検出 / 飼育実験 / 野外調査 / 国際情報交換 |
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| Outline of Final Research Achievements |
Monitoring and biomass estimation of wild organisms are of central importance in conservation biology. However, aquatic organisms are difficult to monitor because of their difficulties in catch and visual observations in the wild.
In this study, we aimed to establish a new molecular method for monitoring and biomass estimation of salmonid species in the wild. We use environmental DNA, which is defined as DNA from environmental media (e.g., water for fish), in order to identify and estimate the amount of wild organisms nearby. Results from our rearing experiments, together with our trials in rivers and the ocean, suggest that environmental DNA we detected indeed reflects the presence/absence and spatio-temporal variation in salmonid species.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] A simple method for preserving environmental DNA in water samples at ambient temperature by addition of cationic surfactant2017
Author(s)
Yamanaka, H., Minamoto, T., Matsuura, J., Sakurai, S., Tsui, S., Motozawa, H., Hongo, M., Sogo, Y., Kakimi, N., Teramura, I., Sugita, M., Baba, M., Kondo, A.
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Journal Title
Limnology
Volume: 17
Issue: 2
Pages: 233-241
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Environmental DNA analysis for estimating the abundance and biomass of stream fish2017
Author(s)
Doi, H., Inui, R., Akamatsu, Y., Kanno, K., Yamanaka, H., Takahara, T., Minamoto, T.
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Journal Title
Freshwater Biology
Volume: 62
Issue: 1
Pages: 30-39
DOI
Related Report
Peer Reviewed
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[Journal Article] Environmental DNA as a ‘Snapshot’ of fish distribution: a case study of Japanese Jack Mackerel in Maizuru Bay, Sea of Japan2016
Author(s)
Yamamoto, Minami, Fukaya, Takahashi, Sawada, Murakami, Tsuji, Hashizume, Kubonaga, Horiuchi, Hongo, Nishida, Okugawa, Fujiwara, Fukuda, Hidaka, Suzuki, Miya, Araki, Yamanaka, Maruyama, Miyashita, Masuda, Minamoto, Kondoh
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Journal Title
PLoS One
Volume: 11
Issue: 3
Pages: 1-18
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] On-site filtration of water samples for environmental DNA analysis to avoid DNA degradation during transportation2016
Author(s)
Yamanaka, H., Motozawa, H., Tsuji, S., Miyazawa, R. C., Takahara, T., Minamoto, T.
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Journal Title
Ecological Research
Volume: 31
Issue: 6
Pages: 963-967
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] Marine fish surveys using environmental DNA2014
Author(s)
Minamoto, T., Masuda, R., Takahashi, K., Maruyama, A., Yamanaka, H., Kasai, A., Kondoh, M.
Organizer
Ecological Societiy of America (ESA) Annual Meeting
Place of Presentation
Sacramento, California, USA
Year and Date
2014-08-10 – 2014-08-15
Related Report
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