Development of label-free and quantitative imaging of cell adhesion
| Project/Area Number |
26650046
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Saitama University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SAZAKI Gen 北海道大学, 低温科学研究所, 教授 (60261509)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Keywords | 光イメージング法 / 細胞接着 / 光干渉 / 原子間力顕微鏡 / 細胞培養基板設計 / がん細胞 / 定量計測 / 細胞・組織 / 生物物理 / 表面・界面 / バイオテクノロジー / 応用光学・量子光工学 / 光学顕微法 |
|---|
| Outline of Final Research Achievements |
In this work, a high-contrast and quantitative imaging method of cell adhesion has been developed by improving instruments and analysis of optical interferometry. In addition, the new method was applied to the study of the quantitative correlation between adhesion and functions of various cells. In particular, the method provided the first quantitative insights into the correlation between adhesion and metastatic ability of cancer cells, which clearly shows the potential of this method for the applications to label-free screening of cell adhesion.
|
Report
(3 results)
Research Products
(8 results)
-
[Journal Article] Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates: An experimental study on the anti-adhesion effect of green-tea catechins2016
Author(s)
Rumi Sakamoto, Eisuke Kakinuma, Kentaro Masuda, Yuko Takeuchi, Kosaku Ito, Kentaro Iketaki, Takahisa Matsuzaki, Seiichiro Nakabayashi, Hiroshi Y. Yoshikawa, Hideaki Yamamoto, Yuko Sato, Takashi Tanii
-
Journal Title
In Vitro Cellular & Developmental Biology -Animal
Volume: 未定
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] Quantitative Evaluation of Cancer Cell Adhesion to Self-Assembled Monolayer-Patterned Substrates by Reflection Interference Contrast Microscopy2016
Author(s)
4)T. Matsuzaki, K. Ito, K. Masuda, E. Kakinuma, R. Sakamoto, K. Iketaki, H. Yamamoto, M. Suganuma, N. Kobayashi, S. Nakabayashi, T. Tanii, H. Y. Yoshikawa
-
Journal Title
Journal of Physical Chemistry B
Volume: 120
Issue: 7
Pages: 1221-1227
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
