Preparation of the first antibody against carotenoids and elucidation of their target molecules in tissues and cells by visualization
Project/Area Number |
26660094
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Bioorganic chemistry
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Research Institution | Tohoku University |
Principal Investigator |
Yamashita Mari 東北大学, (連合)農学研究科(研究院), 教授 (50192430)
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Co-Investigator(Renkei-kenkyūsha) |
KONOKI KEIICHI 東北大学, 大学院農学研究科, 准教授 (40292825)
CHO YUKO 東北大学, 大学院農学研究科, 助教 (60323086)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | カロテノイド / 抗体 / フコキサンチン / レチノイン酸 / 免疫染色 / フコキサンチノール |
Outline of Final Research Achievements |
We prepared antibodies against carotenoids to quantify them and to elucidate their target molecules by visualization of them in tissues and cells. The anti-fucoxanthin and anti-retinoic acid antibodies were prepared by immunization of rabbits by their protein conjugates. These antibodies individually showed specific reactivities to fucoxanthin or retinoic acid. The localization of retinoic acid in mouse neuroblastoma cell line was visualized using this anti-retionoic acid antibody. Retinoic acid was specifically detected in cytoplasm by this antibody when the neuroblastoma cell was treated with retinoic acid to promote neurite outgrowth.
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Report
(3 results)
Research Products
(77 results)
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[Journal Article] Identification of okadaic acid binding protein 2 in reconstituted sponge cell clusters from Halichondria okadai and its contribution to the detoxification of okadaic acid.2015
Author(s)
Keiichi Konoki*, Kayo Okada, Mami Kohama, Hiroki Matsuura, Kaori Saito, Yuko Cho, Goh Nishitani, Tomofumi Miyamoto, Seketsu Fukuzawa, Kazuo Tachibana, Mari Yotsu-Yamashita
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Journal Title
Toxicon
Volume: 108
Pages: 38-45
DOI
Related Report
Peer Reviewed
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[Journal Article] Confirmation of the absence of tetrodotoxin and its analogues in the juveniles of the Japanese fire-bellied newt, Cynops pyrrhogaster, captive-reared from eggs in the laboratory using HILIC-LC-MS.2015
Author(s)
Kudo, Y., Chiba, C., Konoki, K., Cho, Y. and Yotsu-Yamashita, M.
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Journal Title
Toxicon
Volume: 101
Pages: 101-105
DOI
Related Report
Peer Reviewed
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[Journal Article] A high-throughput screen for inhibitors of the prolyl isomerase, Pin1, identifies a seaweed polyphenol that reduces adipose cell differentiation.2014
Author(s)
Mori T, Hidaka M, Ikuji H, Yoshizawa I, Toyohara H, Okuda T, Uchida C, Asano T, Yotsu-Yamashita M, Uchida T
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Journal Title
Biosci. Biotechnol. Biochem.
Volume: 78
Issue: 5
Pages: 832-838
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Analysis of polycavernoside A in the red alga Gracilaria edulis that caused previous fatal food poisonings in Guam and Philippines, The 16th International conference on Harmful Algae2014
Author(s)
○Mari Yotsu-Yamashita, Kosuke Maeta, Yuki Taya, Eri Omizu, Takahide Endo, Yuko Cho, Keiichi Konoki, Mirriam A. Cayme, Yasuo Fukuyo, Sherwood Hall, Takeshi Yasumoto
Organizer
16th International conference on Harmful Algae
Place of Presentation
Wellington, New Zealand
Year and Date
2014-10-27 – 2014-10-31
Related Report
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