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Evolutionary analysis of deep-sea microorganisms incomplete in nuclear membrane structure inhabiting the Wednesday Seamount

Research Project

Project/Area Number 26660176
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Aquatic life science
Research InstitutionTokyo Metropolitan Industrial Technology Research Institute (2016-2017)
Tokyo Medical University (2014-2015)

Principal Investigator

Hachiya Naomi  地方独立行政法人東京都立産業技術研究センター, 開発本部開発第二部バイオ応用技術グループ, 主任研究員 (30408075)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsレーザーマイクロダイセクション法 / 深海微生物 / 蛋白質可溶化 / レーザーマイクロダイセクション / ミトコンドリア / 電子顕微鏡 / 微細構造観察 / 深海
Outline of Final Research Achievements

Since no organisms linking prokaryotes and eukaryotes have been found, it is likely that they have already been culled in the process of evolution or live in places difficult to collect. In this research, we anticipate the possibility of the latter, find out the organisms using microorganisms collected from the deep-sea floor of the Izu-Ogasawara offshore Seamount. Through comprehensive screening by transmission electron microscopy, we found the possibility that microorganisms attached to deep sea weed beetle collected are such organisms. Furthermore, for the experimental analysis, we have developed an advanced system of the laser microdissection system (Advanced-LMD) which easily cut microscopic region of less than 1 μm in diameter. In addition, we constructed a system to recover a large number of samples at once by the capillary phenomenon of the glass capillary.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (33 results)

All 2018 2017 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (9 results) (of which Peer Reviewed: 7 results,  Open Access: 7 results,  Acknowledgement Compliant: 1 results) Presentation (21 results) (of which Int'l Joint Research: 5 results,  Invited: 15 results) Patent(Industrial Property Rights) (2 results)

  • [Int'l Joint Research] Wroclaw Medical University(Poland)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Development of Advanced-Laser Micro Dissection System for Protein Aggregation Diseases2018

    • Author(s)
      Hachiya N and Kozuka Y
    • Journal Title

      Current Trends in Biomedical Engineering & Biosciences

      Volume: 12 Pages: 55584-55591

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comprehensive proteomic profiles of mouse AApoAII amyloid fibrils provide insights into the involvement of lipoproteins in the pathology of amyloidosis2018

    • Author(s)
      Miyahara H, Sawashita J, Ishikawa E, Yang M, Ding X, Liu Y, Hachiya N, Kametani F, Yazaki M, Mori M, Higuchi K
    • Journal Title

      J Proteomics

      Volume: 172 Pages: 111-121

    • DOI

      10.1016/j.jprot.2017.10.003

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Unfoldin, A novel Tool for the Analysis of Protein misfolding or Neurodegerative Diseases2017

    • Author(s)
      Hachiya NS
    • Journal Title

      Journal of Neurology and Neurosurgery

      Volume: 6

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Existence of Unprocessed a Mitochondrial Enzyme: YDL178wp in the Membrane Fraction as an Oligomeric Formation with a Protein-Unfolding Activity2017

    • Author(s)
      Monkawa A., Gessei T., Hachiya N
    • Journal Title

      MOJ Cell Science & Report

      Volume: 4 Pages: 82-86

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] TRIM32-Cytoplasmic-Body Formation Is an ATP-Consuming Process Stimulated by HSP70 in Cells.2017

    • Author(s)
      Kawaguchi Y, Taoka M, Takekiyo T, Uekita T, Shoji I, Hachiya N, Ichimura T.
    • Journal Title

      PLoS One

      Volume: 12(1) Issue: 1 Pages: 1-5

    • DOI

      10.1371/journal.pone.0169436

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The first pure form of Ostertag-type amyloidosis in Japan: a sporadic case of hereditary fibrinogen Aα-chain amyloidosis associated with a novel frameshift variant2017

    • Author(s)
      Yazaki M., Yoshinaga T., Sekijima Y., Nishio S., Kanizawa Y., Kametani F., Miyashita K., Hachiya N., Higuchi K., Ikeda S.
    • Journal Title

      Amyloid

      Volume: 22 Pages: 142-144

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ミスフォールド蛋白解析技術の革新-改良型レーザーマイクロダイセクションシステムの開発2017

    • Author(s)
      紋川亮,八谷如美
    • Journal Title

      別冊医学のあゆみ

      Volume: 1 Pages: 148-149

    • Related Report
      2017 Annual Research Report
  • [Journal Article] ミスフォールド蛋白解析技術の革新-改良型レーザーマイクロダイセクションシステムの開発2017

    • Author(s)
      紋川亮,八谷如美
    • Journal Title

      医学のあゆみ

      Volume: 258 Pages: 748-749

    • Related Report
      2017 Annual Research Report
  • [Journal Article] The pathological and biochemical identification of possible seed-lesions of transmitted transthyretin amyloidosis after domino liver transplantaion.2015

    • Author(s)
      Yoshinaga, T, Yazaki M, Sekijima Y, Kametani F, Miyashita K, Hachiya N, Tanaka T, Koaudo N, Higuchi K, Ikeda S.
    • Journal Title

      J Path: Clin Res 2015

      Volume: 1 Issue: 2 Pages: 1-8

    • DOI

      10.1002/cjp2.36

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Paradigm shift of analytical methods for protein aggregates2017

    • Author(s)
      Hachiya N
    • Organizer
      The Congress of Neurodegeration in Wroclaw Medical University
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 蛋白質凝集疾患研究における改良型レーザーマイクロダイセクション(Advenced-LMD)法の開発と応用2017

    • Author(s)
      八谷如美
    • Organizer
      第56回日本白内障学会総会・第43回水晶体研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 認知症をもっと知る2017

    • Author(s)
      八谷如美
    • Organizer
      東京都健康福祉研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Solubilization of the aggregated-prion protein with the robust protein-unfolding activity if an oligomeric form of YDL178wp/Unfoldin2016

    • Author(s)
      Naomi Hachiya, Miki Fukushima, Kana Miyashita
    • Organizer
      PRION2016
    • Place of Presentation
      一橋ホール
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] HSP70によるTRIM32封入体の形成促進2016

    • Author(s)
      市村徹、河口祐樹、八谷如美
    • Organizer
      平成28年度日本生化学会関東支部例会
    • Place of Presentation
      自治医科大学 医学部 教育・研究棟
    • Related Report
      2016 Research-status Report
  • [Presentation] 分子シャペロンHSP70はアグリゲート形成を促進する2016

    • Author(s)
      市村徹、河口祐樹、八谷如美
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター
    • Related Report
      2016 Research-status Report
  • [Presentation] 改良型 レ ー ザ ー マイクロダイセクションに よるタンパク質解析の新手 法2016

    • Author(s)
      八谷如美
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 生命科学研究の新手法の開発とタンパク質凝集疾患への挑戦2016

    • Author(s)
      八谷如美
    • Organizer
      都医学研セミナー
    • Place of Presentation
      東京都医学総合研究所
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] アミロイド再考2015

    • Author(s)
      八谷如美
    • Organizer
      BMB2015ワークショップ
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Laser microdissection-LC-MS/MSを用いたプロテオーム解析によるアミロイドーシス病型診断の有効性と病早期のアミロイド線維形成機序の解明2015

    • Author(s)
      矢崎正英、亀谷富由樹、八谷如美、池田修一
    • Organizer
      BMB2015ワークショップ
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 八谷如美2015

    • Author(s)
      改良型ダイセクターALMDによるドミノ移植後アミロイドーシス
    • Organizer
      第8回京都大学原子炉実験所セミナー
    • Place of Presentation
      大阪
    • Year and Date
      2015-11-19
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 改良型LMDによるアミロイド解析と新しい生命科学研究手法への展開2015

    • Author(s)
      八谷如美
    • Organizer
      第3回日本アミロイドーシス学術集会
    • Place of Presentation
      東京
    • Year and Date
      2015-08-25
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Adjuster for repeatable targeting of local part of cell at stage of microscope for biochemical analysis.2015

    • Author(s)
      Hashimoto S, Yaguchi Y, Takahashi Y, Hino, H, Miyashita K, Hachiya N
    • Organizer
      The 19th world multiconference on systemics, cybanetics and informatics 2015
    • Place of Presentation
      Orlando, Florida, USA
    • Year and Date
      2015-07-12
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] The first pathological and biochemical identification of seed-lesions of transmitted transthyretin amyloidosis after domino liver transplantation2015

    • Author(s)
      Yoshinaga T, yazaki M, Sekijima Y, Kametani F, Hachiya N, Higuchi K, Ikeda S.
    • Organizer
      第15回International Society of Amyloidosis Symposium
    • Place of Presentation
      Uppsala, Sweden
    • Year and Date
      2015-07-03
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Innovation of Laser Micro Dissection System for the Psychogeriatric study.2015

    • Author(s)
      Naomi Hachiya
    • Organizer
      WPA REGIONAL CONGRESS OSAKA Japan 2015
    • Place of Presentation
      大阪
    • Year and Date
      2015-06-04
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ダイレクトバイオロジーの創成2015

    • Author(s)
      八谷如美
    • Organizer
      第1回ダイレクトバイオロジー研究会
    • Place of Presentation
      東京
    • Year and Date
      2015-02-13
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 認知症克服に向けて2014

    • Author(s)
      八谷如美
    • Organizer
      日本生化学会第87回大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Early Identification and Efficient Therapy of Alzheimer’s Disease2014

    • Author(s)
      Naomi Hachiya
    • Organizer
      ICGP Congress2014
    • Place of Presentation
      つくば
    • Year and Date
      2014-10-01 – 2014-10-04
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] D-amino acid suppresses PrPSc replication in prion-infected neuro2a cells.2014

    • Author(s)
      Kana Miyashita, Kana Nishijima, Naomi Hachiya
    • Organizer
      Asian Pacific Prion Symposium2014
    • Place of Presentation
      Korea
    • Year and Date
      2014-07-06 – 2014-07-07
    • Related Report
      2014 Research-status Report
  • [Presentation] Mitochondrial targeting of prion protein via MSF-Tom70 pathway2014

    • Author(s)
      Hiroki Kato, Naomi Hachiya
    • Organizer
      Asian Pacific Prion Symposium2014
    • Place of Presentation
      Korea
    • Year and Date
      2014-07-06 – 2014-07-07
    • Related Report
      2014 Research-status Report
  • [Presentation] 細胞内異常凝集体の直接解析2014

    • Author(s)
      八谷如美
    • Organizer
      第14回蛋白質科学会
    • Place of Presentation
      横浜
    • Year and Date
      2014-06-25 – 2014-06-27
    • Related Report
      2014 Research-status Report
    • Invited
  • [Patent(Industrial Property Rights)] 生体物質中の構造物等の単離および採取方法2016

    • Inventor(s)
      八谷如美、紋川亮
    • Industrial Property Rights Holder
      東京医科大学、東京都立産業技術研究センター
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-008946
    • Filing Date
      2016-01-20
    • Related Report
      2015 Research-status Report
  • [Patent(Industrial Property Rights)] ラマンイメージングダイセクター2016

    • Inventor(s)
      八谷如美、紋川亮
    • Industrial Property Rights Holder
      東京医科大学、東京都立産業技術研究センター
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-008947
    • Filing Date
      2016-01-20
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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