A novel trial approach for gene-manipulation of tsetse fly
| Project/Area Number |
26670197
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | Kobe Tokiwa University (2015-2017)
Tokyo Medical and Dental University (2014) |
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Principal Investigator |
Suzuki Takashi 神戸常盤大学, 保健科学部, 教授 (70305530)
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| Research Collaborator |
Alexander Egyir-Yawson Ghana Atomic Energy Commission
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | ツェツェバエ / ディフェンシン / アフリカトリパノソーマ原虫 / アフリカトリパノソーマ / 遺伝子組換え / 胎生昆虫 |
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| Outline of Final Research Achievements |
Tsetse flies (Glossina spp.) transmit African trypanosomiasis. In order to effectively control the disease, development of a novel gene manipulating method is expected, which generates the tsetse fly refractory to African trypanosomes. For that purpose, Glossina insectary was firstly set up and preliminary anesthetic system of Glossina was established. Then, anti-microorganism activity of Glossina palpalis defensin（GpDef）was analyzed. The results showed that the molecule possessed a specific anti-trypnosomal activity.
Initial idea of injecting DNA molecules into G. palpalis spermatheca was not successful. Thus in order to see the feasibility of establishing future gene modification system, the following analyses were carried out: a motility and exosome-related molecule of African trypanosome was analyzed and Glossina genome was surveyed in detail for polydnavirus.
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Report
(5 results)
Research Products
(9 results)
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[Journal Article] The Role of Detoxification Enzymes in the Adaptation of the Major Malaria Vector Anopheles gambiae (Giles; Diptera: Culicidae) to Polluted Water.2017
Author(s)
King SA, Onayifeke B, Akorli J, Sibomana I, Chabi J, Manful-Gwira T, Dadzie S, Suzuki T, Wilson MD, Boakye DA, de Souza DK
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Journal Title
Journal of medical entomology
Volume: 54(6)
Issue: 6
Pages: 1674-1683
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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