• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of simple synthetic method of glycoprotein besed on GlycoPEGylation

Research Project

Project/Area Number 26860019
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Chemical pharmacy
Research InstitutionThe Noguchi Institute

Principal Investigator

Goto Kohtaro  公益財団法人野口研究所, 研究部, 研究員 (30321673)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsPEG化 / 糖転移反応 / PEG / Endo-M / GlycoPEGylation / 糖加水分解酵素
Outline of Final Research Achievements

Conventional "GlycoPEGylation" is a synthetic method of glycoproteins attached PEG using glycosyltransferase. This strategy is very difficult to prepare a large amount of target compounds connected PEG owing to the use of glycosyltransferase. In order to resolve this problem, the development of "GlycoPEGylation" using a glycosylhydrolase represented by Endo-M was attempted.
A result of various studies, disaccharide oxazoline derivative which was consist of mannosyl-(β1-4)-N-acetyl glucosamine residues connected two PEG chains to 3’ and 6’ positions of the mannose residue was prepared and used as a glycosyl donor. Furthermore, subsequent enzymatic transglycosylation by using Endo-M was obtained a desired transglycosylated compound.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi