2015 Fiscal Year Final Research Report
Development of simple synthetic method of glycoprotein besed on GlycoPEGylation
Project/Area Number |
26860019
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Chemical pharmacy
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Research Institution | The Noguchi Institute |
Principal Investigator |
Goto Kohtaro 公益財団法人野口研究所, 研究部, 研究員 (30321673)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Keywords | PEG化 / 糖転移反応 |
Outline of Final Research Achievements |
Conventional "GlycoPEGylation" is a synthetic method of glycoproteins attached PEG using glycosyltransferase. This strategy is very difficult to prepare a large amount of target compounds connected PEG owing to the use of glycosyltransferase. In order to resolve this problem, the development of "GlycoPEGylation" using a glycosylhydrolase represented by Endo-M was attempted. A result of various studies, disaccharide oxazoline derivative which was consist of mannosyl-(β1-4)-N-acetyl glucosamine residues connected two PEG chains to 3’ and 6’ positions of the mannose residue was prepared and used as a glycosyl donor. Furthermore, subsequent enzymatic transglycosylation by using Endo-M was obtained a desired transglycosylated compound.
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Free Research Field |
有機化学
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