Mechanism of Ebola viral transcription and replication

• Project/Area Number: 26892028
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Veterinary medical science
• Research Institution: Okinawa Institute of Science and Technology Graduate University
• Principal Investigator: Yukihiko Sugita 沖縄科学技術大学院大学, その他の研究科, 研究員 (00734469)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: エボラウイルス / クライオ電子顕微鏡 / ウイルス学 / 電子顕微鏡

Outline of Final Research Achievements

In this study, we aimed to further understanding of structural basis of Ebola viral transcription and replication by an cryo-electron microscopic analysis of the viral nucleocapsid core, nucleoprotein-RNA complex (NP helix). The reconstructed three-dimensional structure of the NP helix shows that it is formed by a helical assembly of a band of tightly packed NP units. The clearly resolved alpha-helical structures of NP C-terminus are located at the seam between the axial boundaries of the protein band. The structure suggests both a mechanism of self-assembly and reversible structural change of the NP helix during the Ebola viral life cycle.

Research Products

• [Journal Article] Genetic and antigenic characterization of bovine viral diarrhea viruses isolated from cattle in Hokkaido, Japan (2016)
  • Author(s): Abe Y, Tamura T, Torii S, Wakamori S, Nagai M, Mitsuhashi K, Mine J, Fujimoto Y, Nagashima N, Yoshino F, Sugita Y, Nomura T, Okamatsu M, Kida H, Sakoda Y
  • Journal Title: Journal of Veterinary Medical Science Volume: 78 Issue: 1 Pages: 61-70
  • DOI: 10.1292/jvms.15-0186
  • NAID: 130005121585
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Actin-Modulating Protein Cofilin Is Involved in the Formation of Measles Virus Ribonucleoprotein Complex at the Perinuclear Region (2015)
  • Author(s): Koga R, Sugita Y, Noda T, Yanagi Y, Ohno S.
  • Journal Title: Journal of Virology Volume: 89 Issue: 20 Pages: 10524-10531
  • DOI: 10.1128/jvi.01819-15
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Influenza virus-host interactome screen as a platform for antiviral drug development. (2014)
  • Author(s): Watanabe T, Kawakami E, Shoemaker JE, Lopes TJ, Matsuoka Y, Tomita Y, Kozuka-Hata H, Gorai T, Kuwahara T, Takeda E, Nagata A, Takano R, Kiso M, Yamashita M, Sakai-Tagawa Y, Katsura H, Nonaka N, Fujii H, Fujii K, Sugita Y, Noda T, Goto H, Fukuyama S, Watanabe S, Neumann G, Oyama M, Kitano H, Kawaoka Y.
  • Journal Title: Cell Host and Microbe Volume: 16 Issue: 6 Pages: 795-805
  • DOI: 10.1016/j.chom.2014.11.002
  • Peer Reviewed / Open Access
• [Presentation] Cryo-electron tomographic analysis of influenza viral transcription (2016)
  • Author(s): Yukihiko Sugita
  • Organizer: Three Dimensional Electron Microscopy, Gordon Research Conference
  • Place of Presentation: Hong Kong, China
  • Year and Date: 2016-06-19
  • Int'l Joint Research
• [Presentation] エボラウイルスNP helixのクライオ電子顕微鏡解析 (2016)
  • Author(s): 杉田 征彦
  • Organizer: Fifth Negative Strand Virus-Japan Symposium
  • Place of Presentation: 沖縄
  • Year and Date: 2016-01-25
• [Presentation] Cryo-electron microscopic analysis of influenza virus ribonucleoprotein complex during transcription (2015)
  • Author(s): Yukihiko Sugita
  • Organizer: East Asia Joint Symposium 2015
  • Place of Presentation: Okinawa
  • Year and Date: 2015-11-13
  • Int'l Joint Research
• [Presentation] Cryo-electron microscopic analysis of Ebola viral nucleocapcid (2015)
  • Author(s): Yukihiko Sugita
  • Organizer: 4th Negative Strand Virus-Japan Symposium
  • Place of Presentation: 沖縄ラグナガーデンホテル
  • Year and Date: 2015-01-19 – 2015-01-21