マウス生殖系列細胞における生殖顆粒構造の分子機能解析

• 研究領域: 生殖系列の世代サイクルとエピゲノムネットワーク
• 研究課題/領域番号: 20062004
• 研究種目: 特定領域研究
• 配分区分: 補助金
• 審査区分: 生物系
• 研究機関: 京都大学
• 研究代表者: 中辻 憲夫 京都大学, 物質-細胞統合システム拠点, 教授 (80237312)
• 研究分担者: 中馬 新一郎 京都大学, 再生医科学研究所, 准教授 (20378889) ; 中馬 新一郎 京都大学, 再生医科学研究所, 助教 (20378839)
• 研究期間 (年度): 2008 – 2012
• 研究課題ステータス: 完了 (2012年度)
• 配分額: 100,000千円 (直接経費: 100,000千円); 2012年度: 20,000千円 (直接経費: 20,000千円); 2011年度: 20,000千円 (直接経費: 20,000千円); 2010年度: 20,000千円 (直接経費: 20,000千円); 2009年度: 20,000千円 (直接経費: 20,000千円); 2008年度: 20,000千円 (直接経費: 20,000千円)
• キーワード: 発生・分化 / 遺伝学 / 遺伝子 / 動物 / バイオテクノロジー / 発生分化

研究概要

生殖細胞に顕著に観察される構造的特徴に生殖顆粒RNP が挙げられる。同構造は世代、種を越えて広く生殖細胞に観察される事から生殖系列の特性に重要な役割を担うと考えられる。本研究計画ではマウス生殖顆粒の構成分子の解析を進めTDRD1、9 はMILI、MIWI2 と相互作用し精原細胞でpiRNA 経路を介してレトロトランスポゾンを抑制する事、TDRD6、7 は精子細胞の成熟とchromatoid body のRNP リモデリングに重要である事を明らかにすると共にtudor-piwi 経路の新たな構成分子の同定、解析を進めた。

研究成果

• [雑誌論文] GPAT2, a Mitochondrial Acyltransferase in piRNA Biogenesis in Germline Stem Cells (2013)
  • 著者名/発表者名: Shiromoto Y, Kuramochi-Miyagawa S, Daiba A, Chuma S, Katanaya A, Katsumata A, Nishimura K, Ohtaka M,Nakanishi M, Nakamura T, Yoshinaga K, Asada N, Nakamura S, Yasunaga T, Kojima-Kita K, Itou D, Kimura T, Nakano T
  • 雑誌名: RNA 巻: 19 ページ: 803-810
  • 査読あり
• [雑誌論文] GPAT2, a Mitochondrial Acyltransferase, in piRNA Biogenesis in Germline Stem Cells (2013)
  • 著者名/発表者名: Shiromoto Y, Kuramochi-Miyagawa S, Daiba A, Chuma S, Katanaya A, Katsumata A, Nishimura K, Ohtaka M, Nakanishi M, Nakamura T, Yoshinaga K, Asada N, Nakamura S, Yasunaga T, Kojima-Kita K, Itou D, Kimura T, Nakano T
  • 雑誌名: RNA 巻: (in press)
  • 査読あり
• [雑誌論文] A Role for Fkbp6 and the Chaperone Machinery in piRNA Amplification and Transposon Silencing (2012)
  • 著者名/発表者名: Xiol J, Cora E, Koglgruber R, Chuma S, Subramanian S, Hosokawa M, Reuter M, Yang Z, Berninger P, Palencia A, Benes V, Penninger J, Sachidanandam R, Pillai RS
  • 雑誌名: Mol Cell 巻: 47 ページ: 970-979
  • 査読あり
• [雑誌論文] piRNAs and their involvement in male germline development in mice (2012)
  • 著者名/発表者名: Pillai RS, Chuma S
  • 雑誌名: Dev Growth Differ 巻: 54 ページ: 78-92
  • 査読あり
• [雑誌論文] Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing (2012)
  • 著者名/発表者名: Morozumi Y, Ino R, Takaku M, Hosokawa M, Chuma S, Kurumizaka H
  • 雑誌名: Nucleic Acids Res 巻: 40 ページ: 3031-3041
  • 査読あり
• [雑誌論文] MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing (2011)
  • 著者名/発表者名: Reuter M, Berninger P, Chuma S, Shah H, Hosokawa M, Funaya C, Antony C, Sachidanandam R, Pillai RS
  • 雑誌名: Nature 巻: 480 ページ: 264-267
  • 査読あり
• [雑誌論文] Tudor domain containing 7 (Tdrd7) is essential for dynamic ribonucleoprotein (RNP) remodeling of chromatoid bodies during spermatogenesis (2011)
  • 著者名/発表者名: Tanaka T, Hosokawa M, Vagin VV, Reuter M, Hayashi E, Mochizuki AL, Kitamura K, Yamanaka H, Kondoh G, Okawa K, Kuramochi-Miyagawa S, Nakano T, Sachidanandam R, Hannon GJ, Pillai RS, Nakatsuji N, Chuma S
  • 雑誌名: Proc Natl Acad Sci USA 巻: 108 ページ: 10579-10584
  • 査読あり
• [雑誌論文] MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mousegermline (2011)
  • 著者名/発表者名: Watanabe T, Chuma S, Yamamoto Y, Kuramochi-Miyagawa S, Totoki Y, Toyoda A, Hoki Y, Fujiyama A, Shibata T, Sado T, Noce T, Nakano T, Nakatsuji N, Lin H, Sasaki H
  • 雑誌名: Dev Cell 巻: 20 ページ: 364-375
  • 査読あり
• [雑誌論文] TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice (2011)
  • 著者名/発表者名: Yabuta Y, Ohta H, Abe T, Kurimoto K, Chuma S, Saitou M
  • 雑誌名: J Cell Biol 巻: 192 ページ: 781-795
  • 査読あり
• [雑誌論文] MVH in piRNA processing and gene silencing of retrotransposons (2011)
  • 著者名/発表者名: Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Takamatsu K, Chuma S, Kojima-Kita K, Shiromoto Y, Asada N, Toyoda A, Fujiyama A, Totoki Y, Shibata T,Kimura T, Nakatsuji N, Noce T, Sasaki H, Nakano T
  • 雑誌名: Genes Dev 巻: 24 ページ: 887-892
  • 査読あり
• [雑誌論文] Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing (2011)
  • 著者名/発表者名: Morozumi Y, et al
  • 雑誌名: Nucleic Acids Res 巻: 40 号: 7 ページ: 3031-3041
  • DOI: 10.1093/nar/gkr1229
  • 査読あり
• [雑誌論文] Miwi catalysis is required for piRNA amplification-independent LINE1 transposon silencing (2011)
  • 著者名/発表者名: Reuter M, et al
  • 雑誌名: Nature 巻: 480 号: 7376 ページ: 264-267
  • DOI: 10.1038/nature10672
  • 査読あり
• [雑誌論文] MITOPLD Is a Mitochondrial Protein Essential for Nuage Formation and piRNA Biogenesis in the Mouse Germline (2011)
  • 著者名/発表者名: Watanabe T
  • 雑誌名: Dev Cell 巻: 20 ページ: 364-375
  • 査読あり
• [雑誌論文] Functional reconstruction of NANOS3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of NANOS3 (2010)
  • 著者名/発表者名: Yamaji M, Tanaka T, Shigeta M, Chuma S, Saga Y, Saitou M
  • 雑誌名: Reproduction 巻: 139 ページ: 381-393
  • 査読あり
• [雑誌論文] MVH in piRNA processing and gene silencing of retrotransposons. (2010)
  • 著者名/発表者名: Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Takamatsu K, Chuma S, Kojima-Kita K, Shiromoto Y, Asada N, Toyoda A, Fujiyama A, Totoki Y, Shibata T, Kimura T, Nakatsuji N, Noce T, Sasaki H, Nakano T
  • 雑誌名: Genes Dev. 24 ページ: 887-892
  • 査読あり
• [雑誌論文] MVH in piRNA processing and gene silencing of retrotransposons (2010)
  • 著者名/発表者名: Kuramochi-Miyagawa S, et al
  • 雑誌名: Genes Dev 巻: 24 ページ: 887-892
  • 査読あり
• [雑誌論文] Retrotransposon silencing by piRNAs: ping-pong players mark their sub-cellular boundaries (2009)
  • 著者名/発表者名: Chuma S, Pillai RS
  • 雑誌名: PLoS Genet 巻: 5
  • 査読あり
• [雑誌論文] Phenotypic plasticity of mouse spermatogonial stem cells (2009)
  • 著者名/発表者名: Morimoto H, Kanatsu-Shinohara M, Takashima S, Chuma S, Nakatsuji N, Takehashi M, Shinohara T
  • 雑誌名: PLoS One 巻: 4
  • 査読あり
• [雑誌論文] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline (2009)
  • 著者名/発表者名: Shoji M, Tanaka T, Hosokawa M, Reuter M, Stark A, Kato Y, Kondoh G, Okawa K, Chujo T, Suzuki T, Hata K, Martin SL, Noce T, Kuramochi-Miyagawa S, Nakano T, Sasaki H, Pillai RS, Nakatsuji N, Chuma S
  • 雑誌名: Dev Cell 巻: 17 ページ: 775-787
  • 査読あり
• [雑誌論文] Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells (2009)
  • 著者名/発表者名: Kojima K, Kuramochi-Miyagawa S, Chuma S, Tanaka T, Nakatsuji, N, Kimura T, Nakano T
  • 雑誌名: Genes Cells 巻: 14 ページ: 1155-1165
  • 査読あり
• [雑誌論文] Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members (2009)
  • 著者名/発表者名: Vagin VV, Wohlschlegel J, Qu J, Jonsson Z, Huang X, Chuma S, Girard A, Sachidanandam R, Hannon GJ, Aravin AA
  • 雑誌名: Genes Dev 巻: 23 ページ: 1749-1762
  • 査読あり
• [雑誌論文] Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile (2009)
  • 著者名/発表者名: Reuter M, Chuma S, Tanaka T, Franz T, Stark A, Pillai RS
  • 雑誌名: Nat Struct Mol Biol 巻: 16 ページ: 639-646
  • 査読あり
• [雑誌論文] In vitro germ cell differentiation from cynomolgus monkey embryonic stem cells (2009)
  • 著者名/発表者名: Yamauchi K, Hasegawa K, Chuma S, Nakatsuji N, Suemori H
  • 雑誌名: PLoS One 巻: 4
  • 査読あり
• [雑誌論文] Mili interacts with tudor domain-containing protein 1 in regulating spermatogenesis (2009)
  • 著者名/発表者名: Wang J, Saxe JP, Tanaka T, Chuma S, Lin H
  • 雑誌名: Curr Biol 巻: 19 ページ: 640-644
  • 査読あり
• [雑誌論文] Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects (2009)
  • 著者名/発表者名: Takashima S, Takehashi M, Lee J, Chuma S, Okano M, Hata K, Suetake I, Nakatsuji N, Miyoshi H, Tajima S, Tanaka Y, Toyokuni S, Sasaki H, Kanatsu-Shinohara M, Shinohara T
  • 雑誌名: Biol Reprod 巻: 81 ページ: 155-164
  • 査読あり
• [雑誌論文] Ultrastructural characterization of spermatogenesis and its evolutionary conservation in the germline: germinal granules in mammals (2009)
  • 著者名/発表者名: Chuma S, Hosokawa M, Tanaka T, Nakatsuji N
  • 雑誌名: Mol Cell Endocrinol 巻: 306 ページ: 17-23
  • 査読あり
• [雑誌論文] Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. (2009)
  • 著者名/発表者名: Reuter, M, Chuma, S, Tanaka, T, Franz, T, Stark, A, Pillai, RS
  • 雑誌名: Nat.Struct.Mol.Biol. 16 ページ: 639-646
  • 査読あり
• [雑誌論文] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline. (2009)
  • 著者名/発表者名: Shoji, M, Tanaka, T, Hosokawa, M, Reuter, M, Stark, A, Kato, Y, Kondoh, G, Okawa, K, Chujo, T, Suzuki, T, Hata, K, Martin, SL, Noce, T, Kuramochi-Miyagawa, S, Nakano, T, Sasaki, H, Pillai, RS, Nakatsuji, N, Chuma, S
  • 雑誌名: Dev.Cell. 17 ページ: 775-787
  • 査読あり
• [雑誌論文] Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members. (2009)
  • 著者名/発表者名: Vagin, VV, Wohlschlegel, J, Qu, J, Jonsson, Z, Huang, X, Chuma, S, Girard, A, Sachidanandam, R, Hannon, GJ, Aravin, AA
  • 雑誌名: Genes Dev. 23 ページ: 1749-1762
  • 査読あり
• [雑誌論文] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline (2009)
  • 著者名/発表者名: Shoji, M, et al
  • 雑誌名: Dev Cell 17 ページ: 775-787
  • 査読あり
• [雑誌論文] Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members (2009)
  • 著者名/発表者名: Vagin, VV, et al
  • 雑誌名: Genes Dev 23 ページ: 1749-1762
  • 査読あり
• [雑誌論文] Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile (2009)
  • 著者名/発表者名: Reuter, M, et al
  • 雑誌名: Nat Struct Mol Biol 16 ページ: 639-646
  • 査読あり
• [雑誌論文] Mili Interacts with Tudor Domain-Containing Protein 1 in Regulating Spermatogenesis (2009)
  • 著者名/発表者名: Wang, J, et.al.
  • 雑誌名: Curr Biol
  • 査読あり
• [雑誌論文] Homing of mouse spermatogonial stem cells to germline niche depends on beta1-integrin (2008)
  • 著者名/発表者名: Kanatsu-Shinohara M, Takehashi M, Takashima S, Lee J, Morimoto H, Chuma S, Raducanu A, Nakatsuji N, Fassler R, Shinohara T
  • 雑誌名: Cell Stem Cell 巻: 3 ページ: 533-542
  • 査読あり
• [雑誌論文] Production of transgenic rats via lentiviral transduction and xenogeneic transplantation of spermatogonial stem cells (2008)
  • 著者名/発表者名: Kanatsu-Shinohara M, Kato M, Takehashi M, Morimoto H, Takashima S, Chuma S, Nakatsuji N, Hirabayashi M, Shinohara T
  • 雑誌名: Biol Reprod 巻: 79 ページ: 1121-1128
  • 査読あり
• [雑誌論文] Long-term culture of male germline stem cells from hamster testes (2008)
  • 著者名/発表者名: Kanatsu-Shinohara M, Muneto T, Lee J, Takenaka M, Chuma S, Nakatsuji N, Horiuchi T, Shinohara T
  • 雑誌名: Biol Reprod 巻: 78 ページ: 611-617
  • 査読あり
• [雑誌論文] Homing of mouse spermatogonial stem cells to germline niche depends on beta1-integrin. (2008)
  • 著者名/発表者名: Kanatsu-Shinohara, M, Takehashi, M, Takashima, S, Lee, J, Morimoto, H, Chuma, S, Raducanu, A, Nakatsuji, N, Fassler, R, Shinohara, T
  • 雑誌名: Cell Stem Cell. 3 ページ: 533-542
  • 査読あり
• [雑誌論文] Ultrastructural characterization of spermatogenesis and its evolutionary conservation in the germline : Germinal granules in mammals (2008)
  • 著者名/発表者名: Chuma, S, et.al.
  • 雑誌名: Mol Cell Endocrinol
  • 査読あり
• [雑誌論文] Homing of mouse spermatogonial stem cells to germline niche depends on betal-integrin (2008)
  • 著者名/発表者名: Kanatsu-Shinohara, M, et.al.
  • 雑誌名: Cell Stem Cell 3 ページ: 533-542
  • 査読あり
• [雑誌論文] piRNA and spermatogenesis in mice
  • 著者名/発表者名: Chuma S, Nakano T
  • 雑誌名: Philos Trans R Soc Lond B Biol Sci 巻: (in press)
  • 査読あり
• [学会発表] piRNAs in the testis : mammalian tudor genes and germinal granules (2013)
  • 著者名/発表者名: Chuma S
  • 学会等名: International Congress of Andrology
  • 発表場所: Melbourne, Australia(招待講演)
  • 年月日: 2013-02-24
• [学会発表] piRNAs in the testis: mammalian tudor genes and germinal granules (2013)
  • 著者名/発表者名: Chuma S
  • 学会等名: International Congress of Andrology
  • 発表場所: Australia
• [学会発表] Mammalian tudor related genes in the male germline (2011)
  • 著者名/発表者名: Chuma S
  • 学会等名: 44th meeting of society for the study of reproduction
  • 発表場所: Portland, USA
• [学会発表] Germline tudor genes, germinal granules and spermatogenesis in mice (2011)
  • 著者名/発表者名: Chuma S
  • 学会等名: Cold spring harbor asia conference "Developmental Control of Sex, Growth and Cellular Fate"
  • 発表場所: Suzhou, China
• [学会発表] Mammalian tudor related genes in the male germline (2011)
  • 著者名/発表者名: Chuma S
  • 学会等名: 44th meeting of society for the study of reproduction
  • 発表場所: Portland, USA(招待講演)
• [学会発表] Germline tudor genes, germinal granules and spermatogenesis in mice (2010)
  • 著者名/発表者名: Shinichiro Chuma
  • 学会等名: 特定領域研究国際シンポジウム
  • 発表場所: 福岡
• [学会発表] The roles of tudor genes in mammalian spermatogenesis. (2009)
  • 著者名/発表者名: Takashi Tanaka, Mihoko Hosokawa, Norio Nakatsuji, Shinichiro Chuma
  • 学会等名: 第32回日本分子生物学会年会
  • 発表場所: 横浜
• [学会発表] The roles of tudor genes in mammalian spermatogenesis (2009)
  • 著者名/発表者名: Tanaka, T, et al
  • 学会等名: 第32回日本分子生物学会年会
  • 発表場所: 横浜
• [学会発表] Ultrastructural characteristic of spermatogenesis and its evolutionary conservation in the germline (2008)
  • 著者名/発表者名: Chuma S
  • 学会等名: 15th European testis workshop with NAFA annual meeting
  • 発表場所: Naantali, Finland
• [学会発表] Protein and RNA assembly of germinal granules/nuage in the germline (2008)
  • 著者名/発表者名: Chuma S
  • 学会等名: World congress on reproductive biology
  • 発表場所: Hawaii, USA
• [学会発表] Ultrastructural characteristic of spermatogenesis and its evolutionary conservation in the germline. (2008)
  • 著者名/発表者名: Shinichiro Chuma
  • 学会等名: 15th European testis workshop with NAFA annual meeting
  • 発表場所: Naantali, Finland
• [学会発表] Protein and RNA assembly of germinal granules/nuage in the germline. (2008)
  • 著者名/発表者名: Shinichiro Chuma
  • 学会等名: World Congress on Reproductive Biology
  • 発表場所: Hawaii, USA
• [学会発表] Mammalian germ cells and germinal granules/nuage. (2008)
  • 著者名/発表者名: Shinichiro Chuma
  • 学会等名: Germ cell-soma interactions in gonadal development and germ cell tumors (workshops " current trends in biomedicine ")
  • 発表場所: Baeza, Spain
• [学会発表] Role of TDRD9 in male meiosis and regulatory pathway of transposon RNA and DNA methylation. (2008)
  • 著者名/発表者名: Shoji, M, Tanaka, T, Hosokawa, M, Kitamura, K, Kato, Y, Kondoh, G, Okawa, K, Chujyo, T, Suzuki, T, Hata, K, Kuramochi-Miyagawa, S, Nakano, T, Sasaki, H, Chuma, S, Nakatsuji, N
  • 学会等名: Germ Cells, Cold Spring Habor Laboratory Meeting
  • 発表場所: Cold spring harbor, USA
• [学会発表] Role of TDRD9 in male meiosis and regulatory pathway of transposon RNA and DNA methylation (2008)
  • 著者名/発表者名: Shoji, M, et.al.
  • 学会等名: Germ Cells, Cold Spring Habor Laboratory Meeting
  • 発表場所: Cold spring harbor, USA
• [備考]