性ホルモンの軟骨代謝への関与の解析、及びその組織修復への応用

• 研究課題/領域番号: 22791392
• 研究種目: 若手研究(B)
• 配分区分: 補助金
• 研究分野: 整形外科学
• 研究機関: 大阪市立大学
• 研究代表者: 箕田 行秀 大阪市立大学, 大学院・医学研究科・整形外科学, 講師 (90453133)
• 研究期間 (年度): 2009 – 2011
• 研究課題ステータス: 完了 (2011年度)
• 配分額: 3,900千円 (直接経費: 3,000千円、間接経費: 900千円); 2011年度: 2,210千円 (直接経費: 1,700千円、間接経費: 510千円); 2010年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
• キーワード: エストロゲン / 軟骨代謝 / ノックアウトマウス / cre-loxPシステム / ATDC5

研究概要

エストロゲンの軟骨への影響を調べるためには全身性の受容体KOマウスでは不十分であり、4次骨特異的KOマウスの作成が重要であると考え、cre-loxPシステムを用いて、Coloo-Creマウスと、ER-Floxマウスを掛け介わせ、type XI collagenの発現する組織(軟骨)でERが除去されるマウス(軟骨特異的ERa-KOマウス)を作成した。同時に、軟骨前駆細胞殊であるATDC-5や正常マウスの肋軟骨から軟骨細胞を分離し、培養する、そして、それらの細胞の軟骨分化に対するエストロゲンの影響を調査したところ、エストロゲンの濃度依存性に影響を及ぼしている可能性が示唆された。

研究成果

• [雑誌論文] Conventional or navigated total knee arthroplasty affects sagittal component alignment (2012)
  • 著者名/発表者名: Sugama R, Minoda Y, Kobayashi A, Iwaki H, Ikebuchi M, Takaoka K, Nakamura H.
  • 雑誌名: Knee Surg Sports Traumatol Arthrosc
  • 査読あり
• [雑誌論文] Early biological fixation of porous implant coated with paste-retaining recombinant bone morphogenetic protein 2 (2012)
  • 著者名/発表者名: Fukunaga K, Minoda Y, Iwakiri K, Iwaki H, Nakamura H, Takaoka K.
  • 雑誌名: J Arthroplasty 巻: 27 ページ: 143-149
  • 査読あり
• [雑誌論文] Mid-term Mid-term clinical results of alumina medial pivot total knee arthroplasty. pivot total knee arthroplasty (2011)
  • 著者名/発表者名: Iida T, Minoda Y, Kadoya Y, Matsui Y, Kobayashi A, Iwaki H, Ikebuchi M, Yoshida T, Nakamura H.
  • 雑誌名: Knee Surg Sports Traumatol Arthrosc.
  • 査読あり
• [雑誌論文] Sagittal alignment of the lower extremity while standing in female (2011)
  • 著者名/発表者名: Sugama R, Minoda Y, Kobayashi A, lwaki H, Ikebuchi M, Hashimoto Y, Takaoka K, Nakamura H.
  • 雑誌名: Knee Surg Sports Traumatol Arthrosc. 巻: 19 ページ: 74-9
  • 査読あり
• [雑誌論文] Mid-term clinical results of alumina medial pivot total knee arthroplasty (2011)
  • 著者名/発表者名: Iida T, Minoda Y., et al
  • 雑誌名: Knee Sure Sports Traumatol Arthrosc 巻: Nov(Epub ahead of print) 号: 8 ページ: 1514-1519
  • DOI: 10.1007/s00167-011-1734-3
  • 査読あり
• [雑誌論文] Sagittal alignment of the lower extremity while standing in female (2011)
  • 著者名/発表者名: Sugama R, Minoda Y, et al
  • 雑誌名: Knee Surg Sports Traumatol Arthrosc 巻: 19 号: 1 ページ: 74-79
  • DOI: 10.1007/s00167-010-1137-x
  • 査読あり
• [雑誌論文] A cemented mobile-bearing total knee prosthesis prevents peri-prosthetic bone mineral density loss around the femoral component-a matched cohort study (2010)
  • 著者名/発表者名: Minoda Y, Ikebuchi M, Kobayashi A, Iwaki H, Inori F, Nakamura H.
  • 雑誌名: J Bone Joint Surg Br. 巻: 92 ページ: 794-8
  • 査読あり
• [雑誌論文] Comparison of the bone mineral density between porous tantalum and cemented tibial components after total knee arthroplasty. (2010)
  • 著者名/発表者名: Minoda Y, Kobayashi A, Iwaki H, Ikebuchi M, Inori F, Takaoka K.
  • 雑誌名: J Bone Joint Surg Am. 巻: 92 ページ: 700-6
  • 査読あり
• [雑誌論文] Analysis of sex and race and the size and shape of the distal femur using virtual surgery and archived computed tomography images. Barnes CL (2010)
  • 著者名/発表者名: Iwaki H, Minoda Y.
  • 雑誌名: J Surg Orthop Adv. 巻: 19(4) ページ: 200-8
  • 査読あり
• [雑誌論文] Are acetabular component alignment guides for total hip arthroplasty accurate? (2010)
  • 著者名/発表者名: Minoda Y, Ohzono K, Aihara M, Umeda N, Tomita M, Hayakawa K.
  • 雑誌名: J Arthroplasty. 巻: 25 ページ: 986-9
  • 査読あり
• [雑誌論文] The risk of notching the anterior femoral cortex with the use of navigation systems in total knee arthroplasty. (2010)
  • 著者名/発表者名: Minoda Y, Kobayashi A, Iwaki H, Mitsuhiko I, Kadoya Y, Ohashi H, Takaoka K, Nakamura H.
  • 雑誌名: Knee Surg Sports Traumatol Arthrosc. 巻: 18 ページ: 718-22
  • 査読あり
• [雑誌論文] A cemented mobile-bearing total knee replacement prevents periprosthetic loss of bone mineral density around the femoral component : a matched cohort study (2010)
  • 著者名/発表者名: Yukihide Minoda
  • 雑誌名: J Bone Joint Surg Br. 巻: 92 ページ: 794-798
  • 査読あり
• [雑誌論文] Comparison of bone mineral density between porous tantalum and cemented tibial total knee arthroplasty components. (2010)
  • 著者名/発表者名: Yukihide Minoda
  • 雑誌名: J Bone Joint Surg Am. 巻: 92 ページ: 700-706
  • 査読あり
• [雑誌論文] Are acetabular component alignment guides for total hip arthroplasty accurate? (2010)
  • 著者名/発表者名: Yukihide Minoda
  • 雑誌名: J Arthroplasty 巻: 25 ページ: 986-989
  • 査読あり
• [雑誌論文] Analysis of sex and race and the size and shape of the distal femur using virtual surgery and archived computed tomography images. (2010)
  • 著者名/発表者名: Barnes CL, Iwaki H, Minoda Y,
  • 雑誌名: J Surg Orthop Adv. 巻: 19 ページ: 200-208
  • 査読あり
• [雑誌論文] The risk of notching the anterior femoral cortex with the use of navigation systems in total knee arthroplasty. (2010)
  • 著者名/発表者名: Yukihide Minoda
  • 雑誌名: Knee Surg Sports Traumatol Arthrosc. 巻: 18 ページ: 718-722
  • 査読あり
• [雑誌論文] In vivo polyethylene wear particle analysis of improved material and design in TKA (2009)
  • 著者名/発表者名: Minoda Y, Kobayashi A, Iwaki H, Iwakiri K, Inori F, Sugama R, Ikebuchi M, Kadoya Y, Takaoka K.
  • 雑誌名: J Bone Joint Surg Am. 巻: 91 Suppl 6 ページ: 67-73
  • 査読あり
• [雑誌論文] In vivo comparison of wear particles between highly crosslinked polyethylene and conventional polyethylene in the same design of total knee arthroplasties. (2009)
  • 著者名/発表者名: Iwakiri K, Minoda Y, Kobayashi A, Sugama R, Iwaki H, Inori F, Hashimoto Y, Ohashi H, Ohta Y, Fukunaga K, Takaoka K.
  • 雑誌名: J Biomed Mater Res B Appl Biomater. 巻: 91(2) ページ: 799-804
  • 査読あり
• [雑誌論文] Positive nasal culture of methicillin-resistant Staphylococcus aureus is a risk factor for surgical infection in orthopaedic surgeries. (2009)
  • 著者名/発表者名: Yano K. Minoda Y, Sakawa A, Kuwano Y, Kondo K, Fukushima W, Tada K.
  • 雑誌名: Acta Orthop. 巻: 80 ページ: 486-90
  • 査読あり
• [雑誌論文] High incidence of patellar clunk syndrome in a recently designed mobile bearing posterior stabilized total knee prosthesis. (2009)
  • 著者名/発表者名: Fukunaga K, Kobayashi A, Minoda Y, Iwaki H, Hashimoto Y, Takaoka K.
  • 雑誌名: J Bone Joint Surg Br. 巻: 91(4) ページ: 463-8
  • 査読あり
• [雑誌論文] Comparison between highly cross-linked and conventional polyethylene in total knee arthroplasty. (2009)
  • 著者名/発表者名: Minoda Y, Aihara M, Sakawa A, Fukuoka S, Hayakawa K, Tomita M, Umeda N, Ohzono K.
  • 雑誌名: Knee. Knee. 巻: 16(5) ページ: 348-51
  • 査読あり
• [雑誌論文] TKA sagittal alignment with navigation systems and conventional techniques vary only a few degrees. (2009)
  • 著者名/発表者名: Minoda Y, Kobayashi A, Iwaki H, Ohashi H, Takaoka K.
  • 雑誌名: Clin Orthop Relat Res. 巻: 467(4) ページ: 1000-6
  • 査読あり
• [雑誌論文] Range of Motion of Standard and High-Flexion Cruciate Retaining Total Knee Prostheses. (2009)
  • 著者名/発表者名: Minoda Y, Aihara M, Sakawa A, Fukuoka S, Hayakawa K, Ohzono K.
  • 雑誌名: J Arthroplasty. 巻: 24(5) ページ: 674-80
  • 査読あり
• [雑誌論文] Acetabular component orientation in intra-and postoperative positions in total hip arthroplasty. (2009)
  • 著者名/発表者名: Hayakawa K, Minoda Y, Aihara M, Sakawa A, Ohzono K, Tada K.
  • 雑誌名: Arch Orthop Trauma Surg. 巻: 129(9) ページ: 1151-6
  • 査読あり
• [学会発表] Intra-Operative Assessment Of Mid-Flexion Instability In Total Knee Arthropalsty (2012)
  • 著者名/発表者名: Minoda Y, Nakagawa S, Kadoya Y, Kobayashi A, Tomari H, Kondo M, lwaki H, Ikebuchi M, Yoshida T, Matsui Y, Sumitomo A, Sugama R, Mizokawa S, Higuma Y, Noguchi T, Hirakawa H, Nakamura H.
  • 学会等名: Annual meeting of Orthopaedic Reserch Society(ORS)
  • 発表場所: San Francisco, CA, USA.
  • 年月日: 2012-02-04
• [学会発表] The Extension Gap Becomes Tight and Valgus After Implantation of Mobile-bearing Total Knee Prosthesis. (2012)
  • 著者名/発表者名: Minoda Y Nakagawa S, Kadoya Y, Kobayashi A, Tomari H, Kondo M, Iwaki H, Ikebuchi M, Yoshida T, Matsui Y, Sumitomo A, Sugama R, Mizokawa S, Higuma Y, Noguchi T, Hirakawa H, Nakamura H.
  • 学会等名: Annual meeting of Orthopaedic Reserch Society(ORS)
  • 発表場所: San Francisco, CA, USA.
  • 年月日: 2012-02-04
• [学会発表] Operative Technique Affected The Intra-operative Joint Kinematics Of Total Knee Arhtroplasty. (2012)
  • 著者名/発表者名: Minoda Y. Nakagawa S, Kadoya Y, Kobayashi A, Tomari H, Kondo M, Iwaki H, Ikebuchi M, Yoshida T, Matsui Y, Sumitomo A, Sugama R, Mizokawa S, Higuma Y, Noguchi T, Hirakawa H, Nakamura H.
  • 学会等名: Annual meeting of Orthopaedic Reserch Society(ORS)
  • 発表場所: San Francisco, CA, USA.
  • 年月日: 2012-02-04
• [学会発表] In Vivo Wear Particles of Annealed Highly Cross-Linked Polyethylene in Total Hip Arthropalsty. (2012)
  • 著者名/発表者名: Minoda Y Hata K, Iwaki H, Ikebuchi M, Yoshida T, Miyazaki N, Miyake Y, Nakamura H.
  • 学会等名: Annual meeting of Orthopaedic Reserch Society(ORS)
  • 発表場所: San Francisco, CA, USA.
  • 年月日: 2012-02-04
• [学会発表] Mobile-bearing Prosthesis Or Proper Gap Balancing Were Not Predictors For Better Knee Fiexion (2012)
  • 著者名/発表者名: Minoda Y
  • 学会等名: Annual meeting of American Academy of Orthopaedic Surgeons
  • 発表場所: Moscone Convention Center, San Francisco, CA
• [学会発表] Intra-Operative Assessment Of Mid-Flexion Instability In Total Knee Arthropalsty (2012)
  • 著者名/発表者名: Minoda Y
  • 学会等名: 58^<th> Annual meeting of Orthopaedic Reserch Society
  • 発表場所: Moscone Convention Center, San Francisco, CA
• [学会発表] The Extension Gap Becomes Tight and Valgus After Implantation of Mobile-bearing Total Knee Prosthesis (2012)
  • 著者名/発表者名: Minoda Y
  • 学会等名: 58^<th> Annual meeting of Orthopaedic Reserch Society
  • 発表場所: Moscone Convention Center, San Francisco, CA
• [学会発表] Operative Technique Affected The Intra-operative Joint Kinematics Of Total Knee Arhtroplasty (2012)
  • 著者名/発表者名: Minoda Y
  • 学会等名: 58^<th> Annual meeting of Orthopaedic Reserch Society
  • 発表場所: Moscone Convention Center, San Francisco, CA
• [学会発表] In Vivo Wear Particles of Annealed Highly Cross-Linked Polyethylene in Total Hip Arthropalsty (2012)
  • 著者名/発表者名: Minoda Y
  • 学会等名: 58^<th> Annual meeting of Orthopaedic Reserch Society
  • 発表場所: Moscone Convention Center, San Francisco, CA
• [学会発表] In Vivo Wear Particles of Remelted Highly Cross-Linked Polyethylene in Total Hip Arthropalsty (2012)
  • 著者名/発表者名: Minoda Y
  • 学会等名: 58^<th> Annual meeting of Orthopaedic Reserch Society
  • 発表場所: Moscone Convention Center, San Francisco, CA
• [学会発表] Invited lecture ; Future expectation of TKA. (2011)
  • 著者名/発表者名: Minoda Y, Kadoya Y, Kobayashi A, Iwaki H, Iwakiri K, lida T, Matusi Y, Ikebuchi M, Yoshida T, Nakamura H.
  • 学会等名: 24th Annual meeting of the International Society for Technology in Arthroplasty.
  • 発表場所: Brues, Belgium.
  • 年月日: 2011-09-20
• [学会発表] BMD Change Under Bisphosphonate Therapy-Comparison Between Mobile and Fixed Bearing TKA Prostheses-. (2011)
  • 著者名/発表者名: Minoda Y, Hiroyoshi I, Ikebuchi M, Yoshida T, Nakamura H.
  • 学会等名: 24th Annual meeting of the International Society for Technology in Arthroplasty.
  • 発表場所: Brues, Belgium.
  • 年月日: 2011-09-20
• [学会発表] High incidence of anterior femoral cortex notching using navigation system in TKA. (2011)
  • 著者名/発表者名: Minoda Y, Watanabe K, Iwaki H, Ikebuchi M, Yoshida T, lida T, Ikawa T, Hashimoto Y, Nakamura H.
  • 学会等名: Annual meeting of American Association of Orthopaedic Surgeons.
  • 発表場所: San Diego, CA, USA.
  • 年月日: 2011-02-15
• [学会発表] (2011)
  • 著者名/発表者名: Minoda Y, Watanabe K, Iwaki H, Lkebuchi M, Yoshida T, Lida T, Ikawa T, Hashimoto Y, Nakamura H.
  • 学会等名: Annual meeting of American Academy of Orthopaedic Surgeons(AAOS)
  • 発表場所: San Diego, CA, USA.
  • 年月日: 2011-02-07
• [学会発表] Comparison of BMD around the Knee between Trabecular Metal and Cemented Tibial Component ; Four-year Follow-Up Study. (2011)
  • 著者名/発表者名: Minoda Y, Kobayashi A, Ikebuchi M, Iwaki H, Inori F, Hashimoto Y, Yoshida T, lida T, Ikawa T, Yamasaki S, Nakamura H.
  • 学会等名: Annual Meeting of Orthopaedic Research Society.
  • 発表場所: Long Beach, CA, USA.
  • 年月日: 2011-01-13
• [学会発表] Flexion Gap Preparation Opens Both the Extension and Flexion Gap during Total Knee Arthroplasty. (2011)
  • 著者名/発表者名: Minoda Y, Iwaki H, Ikebuchi M, Yoshida T, Iida T, Ikawa T, Yamasaki S, Hirokai N.
  • 学会等名: Annual Meeting of Orthopaedic Research Society.
  • 発表場所: Long Beach, CA, LISA.
  • 年月日: 2011-01-13
• [学会発表] A Cemented Mobile-Bearing Total Knee Arthroplasty Prevents Periprosthetic Loss of Bone Mineral Density around the Femoral Component ; Four-year Follow-Up Study. (2011)
  • 著者名/発表者名: Minoda Y. Kobayashi A, M, Iwaki H. Inori F. Hashimoto Y. Yoshida T, Iida T, Ikawa T, Yamasaki S, Nakamura H.
  • 学会等名: Annual Meeting of Orthopaedic Research Society.
  • 発表場所: Long Beach, CA, USA.
  • 年月日: 2011-01-13
• [学会発表] Future expectation of TKA (2011)
  • 著者名/発表者名: Minoda Y
  • 学会等名: 24^<th> Annual meeting of the International Society for Technology in Arthroplasty, Invited Lecture
  • 発表場所: Concert Building, Brues, Belgium(招待講演)
• [学会発表] BMD Change Under Bisphosphonate Therapy-Comparison Between Mobile and Fixed Bearing TKA Prostheses- (2011)
  • 著者名/発表者名: Minoda Y
  • 学会等名: 24^<th> Annual meeting of the International Society for Technology in Arthroplasty
  • 発表場所: Concert Building, Braes, Belgium
• [学会発表] Mobile Bearing TKA Increased the Peri-Prosthetic Bone Mineral Density Around the Femoral Component. (2010)
  • 著者名/発表者名: Yukihide Minoda
  • 学会等名: 3^<th> Annual meeting of the International Society for Technology in Arthroplasty (ISTA)
  • 発表場所: Dubai