無染色生体組織内での磁気微粒子局在化のＡＤＦ－ＳＴＥＭ解析

• 研究課題/領域番号: 23310075
• 研究種目: 基盤研究(B)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: ナノ材料・ナノバイオサイエンス
• 研究機関: 京都大学
• 研究代表者: 磯田 正二 京都大学, 物質-細胞統合システム拠点, 研究員 (00168288)
• 研究分担者: 高野 幹夫 京都大学, 物質-細胞統合システム拠点 (70068138)
• 研究期間 (年度): 2011-11-18 – 2014-03-31
• 研究課題ステータス: 完了 (2013年度)
• 配分額: 3,120千円 (直接経費: 2,400千円、間接経費: 720千円); 2013年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円); 2012年度: 1,820千円 (直接経費: 1,400千円、間接経費: 420千円)
• キーワード: 無染色可視化 / 暗視野電子顕微鏡 / ソフトマテリアル / メゾスコピック系 / 可視化 / 走査型透過電子顕微鏡 / メゾスコピック / 磁性粒子 / 細胞・組織

研究概要

生体への外部粒子の伝達機構を考える上で、その伝達経路と最終集積部位の同定は生体と粒子系の相互作用の理解に不可欠である。本研究の目的は伝達経路の直接的な空間分布解析を実現する生体試料の高分解能電子顕微鏡解析の方法論を提案することにある。
そのために、軽元素からなる試料でもコントラスト形成可能で、厚い試料でも観察可能である走査型透過電子顕微鏡暗視野法 による無染色解析方法を提案した。結果、 磁性粒子を導入した細胞試料の無染色電顕観察により、微粒子の細胞内での局在化を解析することができた。

研究成果

• [雑誌論文] Quantitative Imaging of Tg in Block Copolymers by Low-Angle Annular Dark-Field Scanning Transmission Electron Microscopy (2013)
  • 著者名/発表者名: Ryotaro Aso, Hiroki Kurata, Takeshi Namikoshi, Tamotsu Hashimoto, Shiao-Wei Kuo, Feng-Chih Chang, Hirokazu Hasegawa, Masahiko Tsujimoto, Mikio Takano, and Seiji Isoda
  • 雑誌名: Macromolecules 巻: 46(21) ページ: 8589-8595
  • 査読あり
• [雑誌論文] Integration of porous coordination polymers and gold nano-rods into core-shell mesoscopic composites towards light-induced molecular release (2013)
  • 著者名/発表者名: Kira Khaletskaya, Julien Reboul, Mikhail Meilikhov, Masashi Nakahama, Stephane Diring, Masahiko Tsujimoto, Seiji Isoda, Franklin Kim, Ken-ichiro Kamei, Roland A. Fischer, Susumu Kitagawa and Shuhei Furukawa
  • 雑誌名: J. American Chemical Society 巻: 135(30) ページ: 10998-11005
  • 査読あり
• [雑誌論文] Highly ordered alignment of polymer chains by host-guest cross-polymerization (2013)
  • 著者名/発表者名: Gaetano Distefano, Takashi Uemura Hirohito Suzuki, Masahiko Tsujimoto, Seiji Isoda, Silvia Bracco, Angiolina Comotti, Piero Sozzani and Susumu Kitagawa
  • 雑誌名: Nature Chem. 巻: 5(4) ページ: 335-341
  • 査読あり
• [雑誌論文] Role of Adsorption Structures of Zn-Porphyrin on TiO2 in Dye-Sensitized Solar Cells Studied by Sum Frequency Generation Vibrational Spectroscopy and Ultrafast Spectroscopy (2013)
  • 著者名/発表者名: Shen Ye, Arunkumar Kathiravan, Hironobu Hayashi, Yujin Tong, Yingyot Infahsaeng, Pavel Chabera, Torbjorn Pascher, Arkady P. Yartsev, Seiji Isoda, Hiroshi Imahori and Villy Sundstrom
  • 雑誌名: J. Physical Chemistry, C. 巻: 117 ページ: 6066-6080
  • 査読あり
• [雑誌論文] Shape Memory Nanopores Induced in Coordination Frameworks by Crystal Downsizing (2013)
  • 著者名/発表者名: Yoko Sakata, Shuhei Furukawa, Mio Kondo, Kenji Hirai, Nao Horike, Yohei Takashima, Hiromitsu Uehara, Nicolas Louvain, Mikhail Meilikhov, Takaaki Tsuruoka, Seiji Isoda, Wataru Kosaka, Osami Sakata, and Susumu Kitagawa
  • 雑誌名: Science 巻: 339(6116) ページ: 193-196
  • 査読あり
• [雑誌論文] Anionic Complexes of MWCNT with Supergiant Cyanobacterial Polyanions (2013)
  • 著者名/発表者名: Okajima, MK.; Maiko K.; Kumar, A.; Fujiwara, A.; Mitsumata, T.; Kaneko, D.; Ogawa, T.; Kurata, H.; Isoda, S.: Kaneko, T.
  • 雑誌名: Biopolymers 巻: 99(1) ページ: 1-9
  • 査読あり
• [雑誌論文] Integration of porous coordination polymers and gold nano-rods into core-shell mesoscopic composites towards light-induced molecular release (2013)
  • 著者名/発表者名: Kira Khaletskaya, Julien Reboul, Mikhail Meilikhov, Masashi Nakahama, Stéphane Diring, Masahiko Tsujimoto, Seiji Isoda, Franklin Kim, Ken-ichiro Kamei, Roland A. Fischer, Susumu Kitagawa and Shuhei Furukawa
  • 雑誌名: Journal of the American Chemical Society 巻: 135 号: 30 ページ: 10998-11005
  • DOI: 10.1021/ja403108x
  • 査読あり
• [雑誌論文] Shape-Memory Nanopores Induced in Coordination Frameworks by Crystal Downsizing (2013)
  • 著者名/発表者名: Yoko Sakata, Shuhei Furukawa, Kenji Hirai, Susuau Kitagawa, et al.
  • 雑誌名: Science 巻: 339 号: 6116 ページ: 193-196
  • DOI: 10.1126/science.1231451
  • 査読あり
• [雑誌論文] Anionic Complexes of MWCNT with Supergiant Cyanobacterial Polyanions (2013)
  • 著者名/発表者名: Okajima, MK.; Maiko K.; Kumar, A.; Fujiwara, A.; Mitsumata, T.; Kaneko, D.; Ogawa, T.; Kurata, H.; Isoda, S.: Kaneko, T.
  • 雑誌名: Biopolymers 巻: 99 号: 1 ページ: 109-109
  • DOI: 10.1002/bip.22129
  • 査読あり
• [雑誌論文] Photodynamic and Photothermal Effects of Semiconducting and Metallic-Enriched Single-Walled Carbon Nanotubes (2012)
  • 著者名/発表者名: Murakami, Tatsuya; Nakatsuji, Hirotaka; Inada, Mami; Matoba, Yoshinori; Umeyama, Tomokazu; Tsujimoto, Masahiko; Isoda, Seiji; Hashida, Mitsuru; Imahori, Hiroshi
  • 雑誌名: J. Am. Chem. Soc. 巻: 134(43) ページ: 17862-17865
  • 査読あり
• [雑誌論文] Modular Design of Domain Assembly in Porous Coordination Polymer Crystals via Reactivity-Directed Crystallization Process (2012)
  • 著者名/発表者名: Fukushima, Tomohiro; Horike, Satoshi; Kobayashi, Hirokazu; Tsujimoto, Masahiko; Isoda, Seiji; Foo, Maw Lin; Kubota, Yoshiki; Takata, Masaki; Kitagawa, Susumu
  • 雑誌名: J. Am. Chem. Soc. 巻: 134(32) ページ: 13341-13347
  • 査読あり
• [雑誌論文] Nanotubes Assisted by Self-assembled Peptides Designed in Conjunction with β-Sheet Formation (2012)
  • 著者名/発表者名: Yasuhiko Hashida, tomokazu Umeyama, Jyunya Mihara, Hiroshi Imahori, Masahimo Tsujimoto, Seiji Isoda and Mikio Takano
  • 雑誌名: J. Pharmaceutical Sciences 巻: 101(9) ページ: 3398-3412
  • 査読あり
• [雑誌論文] Highly photoconducting .-Stacked Polymer accommodated in coordination nanochannels (2012)
  • 著者名/発表者名: Takashi Uemura, Noriyuki Uchida, Atsushi Asano, Akinori Saeki, Shu Seki, Masahiko Tsujimoto, Seiji Isoda, and Susumu Kitagawa
  • 雑誌名: JACS 巻: 134 (20) ページ: 8360-8363
  • 査読あり
• [雑誌論文] Guest-to-Host Structural Transmission for Stimuli-Responsive Adsorption Property (2012)
  • 著者名/発表者名: Nobuhiro Yanai, Takashi Uemura, Masafumi Inoue, Ryotaro Matsuda, Tomohiro Fukushima, Masahiko Tsujimoto, Seiji Isoda, and Susumu Kitagawa
  • 雑誌名: JACS. Communication 巻: 134(10) ページ: 4501-4504
  • 査読あり
• [雑誌論文] A Photoconductive, Thiophene-Fullerene Double-Cable Polymer, Nanorod Device (2012)
  • 著者名/発表者名: Hiroshi Imahori, Shinji Kitaura, Aiko Kira, Hironobu Hayashi, Masayuki Nishi, Kazuyuki Hirao, Seiji Isoda, Masahiko Tsujimoto, Mikio Takano, Zhang Zhe, Yuji Miyato, Kei Noda, Kazumi Matsushige, Kati Stranius, Nikolai V. Tkachenko, Helge Lemmetyinen, Lidong Qin, Sarah J. Hurst and Chad A. Mirkin
  • 雑誌名: J. Phys. Chem., Lett. 巻: 3 ページ: 478-481
  • 査読あり
• [雑誌論文] Development of a Novel Composite Material with Carbon Nanotubes Assisted by Self-assembled Peptides Designed in Conjunction with β-Sheet Formation (2012)
  • 著者名/発表者名: Y. Hashida, T. Umeyama, J. Mihara, H. Imahori, M. Tsujimoto, S. Isoda and M. Takano
  • 雑誌名: Journal of Pharmaceutical Sciences 巻: 101 号: 27 ページ: 3398-3412
  • DOI: 10.1002/anie.201200474
  • 査読あり
• [雑誌論文] Development of a Novel Composite Material with Carbon Nanotubes Assisted by Self-assembled Peptides Designed in Conjunction with β-Sheet Formation (2012)
  • 著者名/発表者名: Yasuhiko Hashida, Tomokazu Umeyama, Jyunya Mihara, Hiroshi Imahori, Masahimo Tsujimoto, Seiji Isoda, Mikio Takano
  • 雑誌名: Journal of Pharmaceutical Sciences 巻: (印刷中)
  • 査読あり
• [雑誌論文] Effects of Fullerene Encapsulation on Photodynamics of Porphyrin-Linked Single-Walled Carbon Nanotubes (2011)
  • 著者名/発表者名: Tomokazu Umeyama, Junya Mihara, Hironobu Hayashi, Naoki Kadota, Vladimir Chukharev, Nikolai V. Tkachenko, Helge Lemmtyinen, Kaname Yoshida, Seiji Isoda and Hirohi Imahori
  • 雑誌名: Chem.Commun 巻: 47(42) ページ: 11781-11783
  • 査読あり
• [雑誌論文] Photoinduced Charge Carrier Dynamics of Zn-Porphyrin-TiO2 Electrodes . The Key Role of Charge Recombination for Solar Cell Performance (2011)
  • 著者名/発表者名: Imahori, Hiroshi; Kang, Soonchul; Hayashi, Hironobu; Haruta, Mitsutaka; Kurata, Hiroki; Isoda, Seiji; Canton, Sophie; Infahsaeng, Yingyot; Kathiravan, Arunkumar; Pascher, Torbjorn; Chabera, Pavel; Yartsev, Arkady and Sundstrom, Villy
  • 雑誌名: The Journal of Physical Chemistry, A 巻: 115(16) ページ: 3679-3690
  • 査読あり
• [雑誌論文] Photophysics and Photoelectrochemical Properties of Nanohybrids Consisting of Fullerene-encapsulated Single-Walled Carbon Nanotubes and Poly-(3-hexylthiophene) (2011)
  • 著者名/発表者名: Noriyasu Tezuka, Tomokazu Umeyama, Yoshihiro Matano, Tetsuya Ogawa, Seiji Isoda, Kati Stranius, Vladimir Chukharev, Nikolai V. Tkachenkod, Helge Lemmetyinen and Hiroshi Imahori
  • 雑誌名: Energy & Environmental Science 巻: 4 ページ: 741-750
  • 査読あり
• [学会発表] Surface Modification of 1D Nanostructures (2013)
  • 著者名/発表者名: Seiji Isoda
  • 学会等名: The 5th Symposium of Promotion Center for Global Materials Research (PCGMR)
  • 発表場所: Tainan, Taiwan
• [学会発表] Quantitative Imaging of Micro-phases in Copolymers by LAADF-STEM (2013)
  • 著者名/発表者名: Ryotaro Aso, Hiroki Kurata, and Seiji Isoda
  • 学会等名: The 13th Pacific Polymer Conference (PPC 2013)
  • 発表場所: Kaohsiung, Taiwan
• [学会発表] Photodynamic and Photothermal Effects of Semiconducting and Metallic-Enriched Single-Walled Carbon Nanotubes for Cancer Cell Killing (2013)
  • 著者名/発表者名: Hirotaka Nakatsuji, Tatsuya Murakami, Mami Inada, Yoshinori Matoba, Tomokazu Umeyama, Masahiko Tsujimoto, Seiji Isoda, Mitsuru Hashida and Hiroshi Imahori
  • 学会等名: 223rd ECS Meeting
  • 発表場所: Toronto, Ontario, Canada
• [学会発表] Molecular imaging by electron microscopy (2013)
  • 著者名/発表者名: S. Isoda and H.Kurata
  • 学会等名: The 30-th Annual Conference of Microscopy Society of Thailand
  • 発表場所: Chanthaburi, Thailand
• [学会発表] Surface Modification of 1D Nanostructures (2013)
  • 著者名/発表者名: Seiji Isoda
  • 学会等名: The 5th Symposium of Promotion Center for Global Materials Research
  • 発表場所: Tainan, Taiwan
  • 招待講演
• [学会発表] Quantitative Imaging of Micro-phases in Copolymers by LAADF-STEM (2013)
  • 著者名/発表者名: Ryotaro Aso, Hiroki Kurata, and Seiji Isoda
  • 学会等名: The 13th Pacific Polymer Conference
  • 発表場所: Kaohsiung, Taiwan
  • 招待講演
• [学会発表] Photodynamic and Photothermal Effects of Semiconducting and Metallic-Enriched Single-Walled Carbon Nanotubes for Cancer Cell Killing (2013)
  • 著者名/発表者名: Hirotaka Nakatsuji, Tatsuya Murakami, Mami Inada, Yoshinori Matoba, Tomokazu Umeyama, Masahiko Tsujimoto, Seiji Isoda, Mitsuru Hashida and Hiroshi Imahori
  • 学会等名: The 223rd ECS Meeting
  • 発表場所: Toronto, Canada
• [学会発表] Molecular imaging by electron microscopy (2013)
  • 著者名/発表者名: S. Isoda and H.Kurata
  • 学会等名: The 30-th Annual Conference of Microscopy Society of Thailand
  • 発表場所: Maneechan Resort, Chanthaburi, Thailand
  • 招待講演
• [学会発表] Local Electronic Structure Analysis for Ca(Sr)FeO2.5 by Site-resolved Energy-loss Near-edge Structures (2012)
  • 著者名/発表者名: M.Haruta, H.Kurata, S.Isoda
  • 学会等名: The 10th Asia-Pacific Microscopy Conference
  • 発表場所: Perth, Australia(招待講演)
  • 年月日: 2012-02-07
• [学会発表] Atomic resolution imaging of organic crystals by STEM (2012)
  • 著者名/発表者名: Mitsutaka Haruta, Seiji Isoda and Hiroki Kurata
  • 学会等名: The 9-th Polish-Japanese-Joint Seminar on Micro and Nano Analysis
  • 発表場所: Sieniawa, Poland
• [学会発表] ADF-STEM Analysis for Soft Materials (2012)
  • 著者名/発表者名: Ryotaro Aso, Masahiko Tsujimoto, Hiroki Kurata, and Seiji Isoda
  • 学会等名: The 9-th Polish-Japanese-Joint Seminar on Micro and Nano Analysis
  • 発表場所: Sieniawa, Poland
• [学会発表] ADF-STEM Analysis for Soft Materials (2012)
  • 著者名/発表者名: R. Aso, M. Tsujimoto, H. Kurata, and S. Isoda
  • 学会等名: The 9-th Polish-Japanese-Joint Seminar on Micro and Nano Analysis
  • 発表場所: Sieniawa Hotel, Sieniawa, Poland
  • 招待講演
• [学会発表] ADF-STEM Imaging of Block Copolymers without Staining (2011)
  • 著者名/発表者名: Ryotaro Aso, Mitsutaka Haruta, Takeshi Namikoshi, Tamotsu Hashimoto, Hiroki Kurata and Seiji Isoda
  • 学会等名: The International Union of Materials Research Societies -International Conference in Asia (IUMRS-ICA)
  • 発表場所: Taipei, Taiwan
• [学会発表] Environmentally-friendly Materials as Photosensitizers for Dye-sensitized Solar Cell (2011)
  • 著者名/発表者名: H. Takano, S. Isoda, M. Tsujimoto, M. Adachi, T. Kiyomura, T. Nemoto and H. Kurata
  • 学会等名: The International Union of Materials Research Societies -International Conference in Asia (IUMRS-ICA)
  • 発表場所: Taipei, Taiwan
• [学会発表] Mesocrystalline Structure of Copper Oxalate Nanocrystals (2011)
  • 著者名/発表者名: Jean-Christophe Valmalette, Tetsuya Ogawa, Julien Romann and Seiji Isoda
  • 学会等名: E-MRS conference
  • 発表場所: Nice, France