小胞体水チャネルの病態生理学的役割の解明

• 研究課題/領域番号: 24659413
• 研究種目: 挑戦的萌芽研究
• 配分区分: 補助金
• 研究分野: 腎臓内科学
• 研究機関: 東京医科歯科大学
• 研究代表者: 佐々木 成 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (60170677)
• 研究期間 (年度): 2012-04-01 – 2014-03-31
• 研究課題ステータス: 完了 (2013年度)
• 配分額: 3,770千円 (直接経費: 2,900千円、間接経費: 870千円); 2013年度: 1,820千円 (直接経費: 1,400千円、間接経費: 420千円); 2012年度: 1,950千円 (直接経費: 1,500千円、間接経費: 450千円)
• キーワード: 水・電解質代謝学 / 水チャネル / 遺伝子改変マウス / アクアポリン / 嚢胞腎 / 小胞体 / トランスジェニックマウス / 免疫染色

研究概要

細胞形質膜に存在し水/イオン/小分子を経上皮的に輸送する輸送体蛋白に比べ、小胞体などの細胞内膜系に存在する輸送体は、研究手法が限られてきたことからその研究は遅れている。しかしながらヒトの病態と深く関わる事も推定され、その研究の進展は急務である。今回、細胞内膜系に存在すると推定されているAQP11のノックアウトマウスが嚢胞腎を発症する分子病態を明らかにした。AQP11は、腎臓では近位尿細管の小胞体に存在し、その欠損が嚢胞腎原因遺伝子PKD1のコードする蛋白PC-1の糖化に障害を与え、PC-1の細胞膜発現低下を通して嚢胞腎が発症することが明らかとなった。

研究成果

• [雑誌論文] Aberrant glycosylation and localization of polycystin-1 cause polycystic kidney in AQP11 knockout mice. (2014)
  • 著者名/発表者名: Inoue Y, Sohara E, Kobayashi K, Chiga M, Rai T, Ishibashi K, Horie S, Xuefeng Su, Jing Zhou, Sasaki S, Uchida S.
  • 雑誌名: J. Am. Soc. Nephrol 巻: in press
• [雑誌論文] Immunolocalization and translocation of aquaporin-5 water channel in sweat glands (2013)
  • 著者名/発表者名: Inoue R, Sohara E, Rai T, Satoh T, Yokozeki H, Sasaki S, Uchida S.
  • 雑誌名: J Dermatol Sci 巻: 70 ページ: 26-33
  • 査読あり
• [雑誌論文] Development of enzyme-linked immunosorbent assays for urinary thiazide-sensitive Na-Cl cotransporter (NCC) measurement (2013)
  • 著者名/発表者名: Isobe K, Mori T, Asano T, Kawaguchi H, Nonoyama S, Kumagai N, Kamada F, Morimoto T, Hayashi M, Sohara E, Rai T, Sasaki S, Uchida S.
  • 雑誌名: Am J Physiol Renal Physiol 巻: 305
  • 査読あり
• [雑誌論文] Chemical library screening for WNK signalling inhibitors using fluorescence correlation spectroscopy (2013)
  • 著者名/発表者名: Mori T, Kikuchi E, Watanabe Y, Fujii S, Ishigami-Yuasa M, Kagechika H, Sohara E, Rai T, Sasaki S, Uchida S.
  • 雑誌名: Biochem J 巻: 455 ページ: 339-45
  • 査読あり
• [雑誌論文] Decrease of WNK4 ubiquitination by disease-causing mutations of KLHL3 through different molecular mechanisms (2013)
  • 著者名/発表者名: Mori Y, Wakabayashi M, Mori T, Araki Y, Sohara E, Rai T, Sasaki S, Uchida S.
  • 雑誌名: Biochem Biophys Res Commun 巻: 439 ページ: 30-4
  • 査読あり
• [雑誌論文] Treatment with 17-allylamino-17-demethoxygeldanamycin ameliorated symptoms of Bartter syndrome type caused by mutated Bsnd in mice (2013)
  • 著者名/発表者名: Nomura N, Kamiya K, Ikeda K, Yui N, Chiga M, Sohara E, Rai T, Sasaki S, Uchida S.
  • 雑誌名: Biochem Biophys Res Commun 巻: 441 ページ: 544-49
  • 査読あり
• [雑誌論文] Hereditary nephrogenic diabetes insipidus in Japanese patients : analysis of 78 families and report of 22 new mutations in AVPR2 and AQP2 (2013)
  • 著者名/発表者名: Sasaki S, Chiga M, Kikuchi E, Rai T, Uchida S.
  • 雑誌名: Clin Exp Nephrol 巻: 17 ページ: 338-344
  • NAID: 10031184239
  • 査読あり
• [雑誌論文] KLHL2 interacts with and ubiquitinates WNK kinases (2013)
  • 著者名/発表者名: Takahashi D, Mori T, Wakabayashi M, Mori Y, Susa K, Zeniya M, Sohara E, Rai T, Sasaki S, Uchida S.
  • 雑誌名: Biochem Biophys Res Commun 巻: 437 ページ: 457-62
  • 査読あり
• [雑誌論文] Impaired KLHL3-mediated ubiquitination of WNK4 causes human hypertension (2013)
  • 著者名/発表者名: Wakabayashi M, Mori T, Isobe K, Sohara E, Susa K, Araki Y, Chiga M, Kikuchi E, Nomura N, Mori Y, Matsuo H, Murata T, Nomura S, Asano T, Kawaguchi H, Nonoyama S, Rai T, Sasaki S, Uchida S.
  • 雑誌名: Cell Rep 巻: 3 ページ: 858-68
  • 査読あり
• [雑誌論文] Dietary salt intake regulates WNK3-SPAK-NKCC1 phosphorylation cascade in mouse aorta through angiotensin II (2013)
  • 著者名/発表者名: Zeniya M, Sohara E, Kita S, Iwamoto T, Susa K, Mori T, Oi K, Chiga M, Takahashi D, Yang SS, Lin SH, Rai T, Sasaki S, Uchida S.
  • 雑誌名: Hypertension 巻: 62 ページ: 872-878
  • 査読あり
• [雑誌論文] Hereditary nephrogenic diabetes insipidus in Japanese patients: analysis of 78 families and report of 22 new mutations in AVPR2 and AQP2. (2013)
  • 著者名/発表者名: Sasaki S, Chiga M, Kikuchi E, Rai T, Uchida S.
  • 雑誌名: Clin Exp Nephrol. 巻: 3 号: 3 ページ: 338-44
  • DOI: 10.1007/s10157-012-0726-z
  • NAID: 10031184239
  • 査読あり
• [雑誌論文] Immunolocalization and translocation of aquaporin-5 water channel in sweat glands. (2013)
  • 著者名/発表者名: Inoue R, Sohara E, Rai T, Satoh T, Yokozeki H, Sasaki S, Uchida S.
  • 雑誌名: J Dermatol Sci. 巻: 70 号: 1 ページ: 26-33
  • DOI: 10.1016/j.jdermsci.2013.01.013
  • 査読あり
• [雑誌論文] Treatment with 17-allylamino-17-demethoxygeldanamycin ameliorated symptoms of Bartter syndrome type caused by mutated Bsnd in mice. (2013)
  • 著者名/発表者名: Nomura N, Kamiya K, Ikeda K, Yui N, Chiga M, Sohara E, Rai T, Sasaki S, Uchida S.
  • 雑誌名: Biochem Biophys Res Commun. 巻: 441 号: 3 ページ: 544-49
  • DOI: 10.1016/j.bbrc.2013.10.129
  • 査読あり
• [雑誌論文] The impact of hyporesponsiveness to erythropoietin-stimulating agents on time-dependent mortality risk among CKD stage 5D patients: a single-center cohort study. (2013)
  • 著者名/発表者名: Ishigami J
  • 雑誌名: Clin. Exp. Nephrol. 巻: 17 号: 1 ページ: 106-114
  • DOI: 10.1007/s10157-012-0659-6
  • 査読あり
• [雑誌論文] Characterization of AQP-2 gene mutation (R254Q) in a family with dominant nephrogenic DI.. (2013)
  • 著者名/発表者名: Shida Y
  • 雑誌名: Pediatr Int. 巻: 55 号: 1 ページ: 105-107
  • DOI: 10.1111/j.1442-200x.2012.03614.x
  • 査読あり
• [雑誌論文] Impaired KLHL3-mediated ubiquitination of WNK4 causes human hypertension. (2013)
  • 著者名/発表者名: Wakabayashi M
  • 雑誌名: Cell Rep. 巻: 28 号: 3 ページ: 858-868
  • DOI: 10.1016/j.celrep.2013.02.024
  • 査読あり
• [雑誌論文] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates its ubiquitination (2012)
  • 著者名/発表者名: Hossain Khan MZ, Sohara E, Ohta A, Chiga M, Inoue Y, Isobe K, Wakabayashi M, Oi K, Rai T, Sasaki S, Uchida S.
  • 雑誌名: Biochem. Biophys. Res. Commun 巻: 425 ページ: 456-61
  • 査読あり
• [雑誌論文] Phosphatidylinositol 3-kinase/Akt signaling pathway activates the WNK-OSR1/SPAK-NCC phosphorylation cascade in hyperinsulinemic db/db mice (2012)
  • 著者名/発表者名: Nishida H, Sohara E, Nomura N, Chiga M, Alessi DR, Rai T, Sasaki S, Uchida S.
  • 雑誌名: Hypertension 巻: 60 ページ: 981-90
  • 査読あり
• [雑誌論文] A minor role of WNK3 in regulating phosphorylation of renal NKCC2 and NCC co-transporters in vivo (2012)
  • 著者名/発表者名: Oi K, Sohara E, Rai T, Misawa M, Chiga M, Alessi DR, Sasaki S, Uchida S.
  • 雑誌名: Biol.Open 巻: 1 ページ: 120-7
  • 査読あり
• [雑誌論文] Effect of heterozygous deletion of WNK1 on the WNK-OSR1/ SPAK-NCC/NKCC1/NKCC2 signal cascade in the kidney and blood vessels (2012)
  • 著者名/発表者名: Susa K, Kita S, Iwamoto T, Yang SS, Lin SH, Ohta A, Sohara E, Rai T, Sasaki S, Alessi DR, Uchida S.
  • 雑誌名: Clin Exp. Nephrol 巻: 16 ページ: 530-8
  • NAID: 10031058026
  • 査読あり
• [雑誌論文] WNK-OSR1/SPAK-NCC signal cascade has circadian rhythm dependent on aldosterone (2012)
  • 著者名/発表者名: Susa K, Sohara E, Isobe K, Chiga M, Rai T, Sasaki S, Uchida S.
  • 雑誌名: Biochem. Biophys. Res. Commun 巻: 427 ページ: 743-7
  • 査読あり
• [雑誌論文] Does aβ2-adrenergic receptor-WNK4-Na-Cl co-transporter signal cascade exist in the in vivo kidney? (2012)
  • 著者名/発表者名: Uchida S, Chiga M, Sohara E, Rai T, Sasaki S.
  • 雑誌名: Nat. Med 巻: 18 ページ: 1324-5
  • 査読あり
• [雑誌論文] Close Association of Aquaporin-2 Internalization with Caveolin-1 (2012)
  • 著者名/発表者名: Aoki T., 他
  • 雑誌名: Ａｃｔａ Ｈｉｓｔｏｃｈｅｍｉｃａ ｅｔ Ｃｙｔｏｃｈｅｍｉｃａ 巻: 45 号: 2 ページ: 139-146
  • DOI: 10.1267/ahc.12003
  • NAID: 130001854241
  • ISSN: 0044-5991, 1347-5800
  • 査読あり
• [雑誌論文] Involvement of aquaporin-7 in the cutaneous primary immune response through modulation of antigen uptake and migration in dendritic cells. (2012)
  • 著者名/発表者名: Hara-Chikuma M
  • 雑誌名: FASEB J. 巻: 26 号: 1 ページ: 211-218
  • DOI: 10.1096/fj.11-186627
  • 査読あり
• [雑誌論文] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates its ubiquitination. (2012)
  • 著者名/発表者名: Hossain Khan MZ
  • 雑誌名: Biochem. Biophys. Res. Commun. 巻: 425 号: 2 ページ: 456-461
  • DOI: 10.1016/j.bbrc.2012.07.124
  • 査読あり
• [雑誌論文] Effects of sevelamer hydrochloride on mortality, lipid abnormality and arterial stiffness in hemodialyzed patients: a propensity-matched observational study. (2012)
  • 著者名/発表者名: Iimori S
  • 雑誌名: Clin. Exp. Nephrol. 巻: 16 号: 6 ページ: 930-937
  • DOI: 10.1007/s10157-012-0640-4
  • 査読あり
• [雑誌論文] Diagnostic usefulness of bone mineral density and biochemical markers of bone turnover in predicting fracture in CKD stage 5D patients - a single-center cohort study. (2012)
  • 著者名/発表者名: Iimori S
  • 雑誌名: Nephrol. Dial. Transplant. 巻: 27 号: 1 ページ: 345-351
  • DOI: 10.1093/ndt/gfr317
  • 査読あり
• [雑誌論文] Applicability of fibroblast growth factor 23 for evaluation of risk of vertebral fracture and chronic kidney disease-mineral bone disease in elderly chronic kidney disease patients. (2012)
  • 著者名/発表者名: Kanda E
  • 雑誌名: BMC Nephrol. 巻: 13 号: 1 ページ: 122-122
  • DOI: 10.1186/1471-2369-13-122
  • 査読あり
• [雑誌論文] Murine colon proteome and characterization of the protein pathways. (2012)
  • 著者名/発表者名: Magdeldin S
  • 雑誌名: BioData Min. 巻: 5 号: 1 ページ: 11-11
  • DOI: 10.1186/1756-0381-5-11
  • 査読あり
• [雑誌論文] Anti-centromere antibody-positive subjects presenting with hypertensive emergency and renal dysfunction in the absence of skin manifestations: a variant of systemic sclerosis or a novel entity? (2012)
  • 著者名/発表者名: Mandai S
  • 雑誌名: Intern.Med. 巻: 51 ページ: 1567-1572
  • 査読あり
• [雑誌論文] Phosphatidylinositol 3-kinase/Akt signaling pathway activates the WNK-OSR1/SPAK-NCC phosphorylation cascade in hyperinsulinemic db/db mice. (2012)
  • 著者名/発表者名: Nishida H
  • 雑誌名: Hypertension 巻: 60 号: 4 ページ: 981-990
  • DOI: 10.1161/hypertensionaha.112.201509
  • 査読あり
• [雑誌論文] Accumulation of autofluorescent storage material in brain is accelerated by ischemia in chloride channel 3 gene-deficient mice. (2012)
  • 著者名/発表者名: Ohtaki H
  • 雑誌名: J. Neurosci. Res. 巻: 90 号: 11 ページ: 2163-2172
  • DOI: 10.1002/jnr.23110
  • 査読あり
• [雑誌論文] A minor role of WNK3 in regulating phosphorylation of renal NKCC2 and NCC co-transporters in vivo (2012)
  • 著者名/発表者名: Oi K
  • 雑誌名: Biology Open 巻: Vol.1 号: 2 ページ: 120-127
  • DOI: 10.1242/bio.2011048
  • 査読あり
• [雑誌論文] Aquaporin 2: from its discovery to molecular structure and medical implications. (2012)
  • 著者名/発表者名: Sasaki S.
  • 雑誌名: Mol. Aspects. Med. 巻: 33 号: 5-6 ページ: 535-546
  • DOI: 10.1016/j.mam.2012.03.004
  • 査読あり
• [雑誌論文] Effect of heterozygous deletion of WNK1 on the WNK-OSR1/ SPAK-NCC/NKCC1/NKCC2 signal cascade in the kidney and blood vessels. (2012)
  • 著者名/発表者名: Susa K
  • 雑誌名: Clin. Exp. Nephrol. 巻: 16 ページ: 530-538
  • 査読あり
• [雑誌論文] WNK-OSR1/SPAK-NCC signal cascade has circadian rhythm dependent on aldosterone. (2012)
  • 著者名/発表者名: Susa K
  • 雑誌名: Biochem. Biophys. Res. Commun. 巻: 427 号: 4 ページ: 743-747
  • DOI: 10.1016/j.bbrc.2012.09.130
  • 査読あり
• [雑誌論文] Does a β2-adrenergic receptor-WNK4-Na-Cl co-transporter signal cascade exist in the in vivo kidney? (2012)
  • 著者名/発表者名: Uchida S
  • 雑誌名: Nat. Med. 巻: 18 号: 9 ページ: 1324-1325
  • DOI: 10.1038/nm.2809
  • 査読あり
• [雑誌論文] Severe hyperparathyroidism in a pre-dialysis chronic kidney disease patient treated with a very low protein diet (2011)
  • 著者名/発表者名: Ohta E
  • 雑誌名: J. Bone Miner. Metab. 巻: (published online) 号: 2 ページ: 238-242
  • DOI: 10.1007/s00774-011-0320-6
  • 査読あり
• [雑誌論文] Daily variance of urinary excretion of AQP2 determined by sandwich ELISA method (2011)
  • 著者名/発表者名: Sasaki S
  • 雑誌名: Clin.Exp.Nephrol. 巻: (published online) 号: 3 ページ: 406-410
  • DOI: 10.1007/s10157-011-0574-2
  • NAID: 10030808445
  • 査読あり
• [学会発表] Analyses of KLHL3 mutants that cause Pseudohypoaldosteronism type II (2013)
  • 著者名/発表者名: Mori Y, Wakabayashi M, Mori T, Araki Y, Sohara E, Rai T, Sasaki S, Uchida S.
  • 学会等名: The 46^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-09
• [学会発表] KLHL2 interacts with and ubiquitinates WNK kinases (2013)
  • 著者名/発表者名: Takahashi D, Mori T, Wakabayashi M, Mori Y, Susa K, Zeniya M, Sohara E, Rai T, Sasaki S, Uchida S.
  • 学会等名: The 46^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-09
• [学会発表] Impaired KLHL3-mediated ubiquitination of WNK4 activates OSR1 and SPAK kinases-NaCl cotransporter (NCC) signaling and causes hypertension (2013)
  • 著者名/発表者名: Wakabayashi M, Mori T, Isobe K, Sohara E, Susa K, Araki Y, Chiga M, Kikuchi E, Nomura N, Mori Y, Rai T, Sasaki S, Uchida S.
  • 学会等名: The 46^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-09
• [学会発表] WNK3 Regulates Blood Pressure Through the Regulation of Vascular OSR1/SPAK-NKCC1 Phosphorylation Cascade (2013)
  • 著者名/発表者名: Zeniya M, Sohara E, Kita S, Iwamoto T, Susa K, Mori T, Oi K, Chiga M, Takahashi D, Yang SS, Lin SH, Rai T, Sasaki S, Uchida S.
  • 学会等名: The 46^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-09
• [学会発表] Aberrant Glycosylation and Localization of Polycystin-1 Cause Polycystic Kidney in AQP11-Knockout Mice (2013)
  • 著者名/発表者名: Inoue Y, Sohara E, Kobayashi K, Rai T, Ishibashi K, Horie S, Su X, Zhou J, Sasaki S, Uchida S.
  • 学会等名: The 46^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-07
• [学会発表] Clinical Significance of Urinary Thiazide-Sensitive Na-Cl Cotransporter (NCC) Measurement by Newly Development Enzyme-Linked Immunosorbent Assays (2013)
  • 著者名/発表者名: Isobe K, Mori T, Sohara E, Rai T, Sasaki S, Uchida S.
  • 学会等名: The 46^<th> Annual meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-07
• [学会発表] Chemical library screening for WNK signaling inhibitors by using fluorescent correlation spectroscopy (2013)
  • 著者名/発表者名: Mori T, Eriko K, Sohara E, Rai T, Sasaki S, Uchida S.
  • 学会等名: The 46^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-07
• [学会発表] Low salt intake decreased transcription and protein level of KLHL3 in mouse kidney (2013)
  • 著者名/発表者名: Susa K, Sohara E, Zeniya M, Rai T, Sasaki S, Uchida S.
  • 学会等名: The 46^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-07
• [学会発表] Ser-261 phosphorylation is an endocytic signal of aquaporin-2 water channel (2013)
  • 著者名/発表者名: Yui N, Uchida S, Sasaki S.
  • 学会等名: The 46^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-07
• [学会発表] Aberrant Glycosylation and Localization of Polycystin-1 Cause Polycystic Kidney in AQP11-Knockout Mice. (2013)
  • 著者名/発表者名: Inoue Y, Sohara E, Kobayashi K, Rai T, Ishibashi K, Horie S, Su X, Zhou J, Sasaki S, Uchida S.
  • 学会等名: The 46th Annual meeting of American Society of Nephrology
  • 発表場所: アトランタ, アメリカ合衆国
• [学会発表] Ser-261 phosphorylation is an endocytic signal of aquaporin-2 water channel. (2013)
  • 著者名/発表者名: Yui N, Uchida S, Sasaki S.
  • 学会等名: The 46th Annual meeting of American Society of Nephrology
  • 発表場所: アトランタ, アメリカ合衆国
• [学会発表] Clinical Significance of Urinary Thiazide-Sensitive Na-Cl Cotransporter (NCC) Measurement by Newly Development Enzyme-Linked Immunosorbent Assays. (2013)
  • 著者名/発表者名: Isobe K, Mori T, Sohara E, Rai T, Sasaki S, Uchida S.
  • 学会等名: The 46th Annual meeting of American Society of Nephrology
  • 発表場所: アトランタ, アメリカ合衆国
• [学会発表] Hereditary nephrogenic diabetes insipidus (NDI) in Japanese patients : Analysis of 73 families (2012)
  • 著者名/発表者名: Sasaki S, Chiga M, Kikuchi E, Uchida S.
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 2012-11-02
• [学会発表] WNK4-OSR1/SPAK-NCC signal cascade has circadian rhythm dependent on aldosterone (2012)
  • 著者名/発表者名: Susa K, Sohara E, Isobe K, Chiga M, Rai T, Sasaki S, Uchida S.
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 2012-11-02
• [学会発表] Chemical library screening for direct SPAK inhibitors by a newly developed ELISA system (2012)
  • 著者名/発表者名: Kikuchi E, Mori T, Isobe K, Sohara E, Rai T, Sasaki S, Uchida S.
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 2012-11-01
• [学会発表] Generation and analysis of WT-WNK4 transgenic mice revealed the physiological role of WNK4 (2012)
  • 著者名/発表者名: Mori T, Sohara E, Rai T, Sasaki S, Uchida S.
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 2012-11-01
• [学会発表] The size and viability of mesothelial cells affect peritoneal membrane function. (2012)
  • 著者名/発表者名: Akazawa M
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates its ubiquitination. (2012)
  • 著者名/発表者名: Hossain Khan MZ
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Impaired trafficking of polycystin-1 may be a key mechanism of cyst formation in the aquaporin-11 knockout mouse. (2012)
  • 著者名/発表者名: Inoue Y
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Development of sandwich enzyme linked immunosorbent assay (ELISA) for measurement of urinary total and phosphorylated Na-Clcotransporter (NCC) protein. (2012)
  • 著者名/発表者名: Isobe K
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Rate of change in fibroblast growth factor 23 level predicts the progression of chronic kidney disease in elderly patients. (2012)
  • 著者名/発表者名: Kanda E
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] A new high-performance liquid chromatography showed that icodextrin dialysate favorably improved lipid profiles in peritoneal dialysis patients. (2012)
  • 著者名/発表者名: Kanda E
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Chemical library screening for direct SPAK inhibitors by a newly developed ELISA system. (2012)
  • 著者名/発表者名: Kikuchi E
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Generation and analysis of WT-WNK4 transgenic mice revealed the physiological role of WNK4. (2012)
  • 著者名/発表者名: Mori T
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Hereditary nephrogenic diabetes insipidus (NDI) in Japanese patients: Analysis of 73 families. (2012)
  • 著者名/発表者名: Sasaki S
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] WNK4-OSR1/SPAK-NCC signal cascade has circadian rhythm dependent on aldosterone. (2012)
  • 著者名/発表者名: Susa K
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Dietary salt intake and angiotensin II regulates WNK-SPAK-NKCC1 phosphorylation cascade in mouse aorta. (2012)
  • 著者名/発表者名: Zeniya M
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [図書] Chapter 4. Regulation of Water Balance: Urine Concentration and Dilution. Diseases of the Kidney & Urinary Tract, Ninth Edition (2012)
  • 著者名/発表者名: Noda Y, Sasaki S.
  • 出版者: Lippincott Williams & Wilkins
• [備考] 東京医科歯科大学腎臓内科学ホームページ
  • URL: http://www.tmd.ac.jp/grad/kid/kid-J.htm
• [備考] 東京医科歯科大学腎臓内科学
  • URL: http://www.tmd.ac.jp/grad/kid/kid-J.htm
• [備考] 東京医科歯科大学腎臓内科学
  • URL: http://www.tmd.ac.jp/grad/kid/kid-J.htm