研究課題/領域番号 |
16K11525
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研究機関 | 日本大学 |
研究代表者 |
Cueno Marni 日本大学, 歯学部, 専修研究員 (20569967)
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研究分担者 |
落合 智子 (栗田智子) 日本大学, 松戸歯学部, 教授 (20130594)
落合 邦康 日本大学, 歯学部, 特任教授 (50095444)
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研究期間 (年度) |
2016-04-01 – 2019-03-31
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キーワード | vaccine design / oral vaccination |
研究実績の概要 |
Objectives: (1) To design a gel-encapsulated gingival vaccine in silico; (2) to determine Langerhan cells (LC) within the sulcular epithelium; and (3) to optimize the vaccine conditions for the other vaccination routes (nasal and sublingual). Methods: For vaccine design, special computer software was used in order to structurally visualize our proposed trivalent gingival vaccine. For LC determination, we utilized both a histological (staining) and biochemical (FceRIg) approach to estimate LC number. For optimization of other vaccination routes, ideal experimental animal (rat or mouse) and appropriate antigen concentration to induce an antibody response are both performed. Results: For vaccine design, in silico determination of gel-encapsulated antigens [H1N1 nucleoprotein, H5N1 hemagglutinin, dengue envelope-2 protein] were established. Gel encapsulation seems to suggest that all antibody epitopes for each of the antigens are unblocked. This would insinuate that an antibody response can be induced. For LC determination, there was difficulty establishing the LC number along the sulcular epithelium using a histological approach. An ELISA system targeting a common LC marker (FceRIg) was used instead since FceRIg levels would correlate to LC number. For optimization of other vaccination routes, conditions for sublingual vaccination route have been optimized. Optimization for the nasal vaccination route is still ongoing.
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現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We were able to follow the scheduled plan as indicated in our earlier proposal. For vaccine design, in silico determination of gel-encapsulated antigens were fully made. For LC determination, although there was difficulty establishing the LC number along the sulcular epithelium using a histological approach, using an ELISA system was found effective. For optimization of other vaccination routes, conditions for sublingual vaccination route was optimized while optimization for the nasal vaccination route is still ongoing.
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今後の研究の推進方策 |
Objectives: To optimize the antigen:xanthan gel ratio components based on our earlier computer analyses; (2) to quantify the number of adaptive lymphocytes prior to vaccination; and (3) to optimize other possible vaccination routes.
Methods: For optimizing the antigen:xanthan gel ratio components, ideal antigen and xanthan gel concentrations in combination together will be determined. For quantifying adaptive lymphocyte number, FceRIg levels and another possible biochemical marker will be used and quantified using ELISA. For optimizing other possible vaccination routes (oral, intradermal), selected antigens from the current year [H1N1 nucleoprotein, H5N1 hemagglutinin, dengue envelope-2 protein] will be administered orally and intradermally to a selected experimental animal.
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次年度使用額が生じた理由 |
Shifting to a biochemical approach from a histological approach in order to determine Langerhan Cell (LC) number in the rat sulcular epithelium made LC estimation more accurate. This allowed us to save on time while maintaining efficiency.
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次年度使用額の使用計画 |
The budget for the current fiscal year plus the money we obtained from the previous year will be used for optimizing the antigen:xanthan gel component ratio, determine the adaptive lymphocyte number through biochemical markers, and to optimize other possible vaccination routes.
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