抗がん剤感受性に関わるゲノム異常の同定

2011 年度 研究成果報告書

• 研究課題/領域番号: 21200001
• 研究種目: 新学術領域研究(研究課題提案型)
• 配分区分: 補助金
• 研究分野: ゲノム情報科学; 生体生命情報学
• 研究機関: 大分大学
• 研究代表者: 守山 正胤 大分大学, 医学部, 教授 (90239707)
• 研究分担者: 白尾 国昭 大分大学, 医学部, 教授 (10467996) ; 竹内 一郎 名古屋工業大学, 工学(系)研究科(研究院), 准教授 (40335146)
• 研究期間 (年度): 2009 – 2011
• キーワード: バイオインフォマティクス / アレイCGH / メチル化 / トランスクリプトーム / microRNA / 抗がん剤感受性 / がん化学療法

研究概要

胃癌、腎癌、大腸癌のゲノム異常解析（ゲノムコピー数異常解析、mRNA発現解析、microRNA発現解析）を行い、それらのデータをバイオインファマテイクスにより解析することによって、１）miR-375が胃癌の癌抑制microRNAであることを発見した。２）腎癌においてmiR-210が発現亢進することでaneuploidyをもたらすことを発見した。３）SAV1が腎癌の癌抑制遺伝子であることを発見した。

研究成果

• [雑誌論文] Integrated analysis of genetic and epigenetic alterations reveals CpG island methylator phenotype associated with distinct clinical characters of lung adenocarcinoma. (2012)
  • 著者名/発表者名: Shinjo K, Okamoto Y, An B, Yokoyama T, Takeuchi I, Fujii M, Osada H, Usami N, Hasegawa Y, Ito H, Hida T, Fujimoto N, Kishimoto T, Sekido Y, Kondo Y.
  • 雑誌名: Carcinogenesis 巻: (Epub ahead of print)
• [雑誌論文] Pharmacokinetic parameters from 3-Tesla DCE-MRI as surrogate biomarkers of antitumor effects of bevacizumab plus FOLFIRI in colorectal cancer with liver metastasis. (2012)
  • 著者名/発表者名: Hirashima Y, Yamada Y, Tateishi U, Kato K, Miyake M, Horita Y, Akiyoshi K, Takashima A, Okita N, Takahari D, Nakajima T, Hamaguchi T, Shimada Y, Shirao K.
  • 雑誌名: Int J Cancer. 巻: 130(10) ページ: 2359-65
• [雑誌論文] Genomic profiling of renal cell carcinoma in patients with end-stage renal disease. (2012)
  • 著者名/発表者名: Inoue T, Matsuura K, Yoshimoto T, Nguyen LT, Tsukamoto Y, Nakada C, Hijiya N, Narimatsu T, Nomura T, Sato F, Nagashima Y, Kashima K, Hatakeyama S, Ohyama C, Numakura K, Habuchi T, Nakagawa M, Seto M, Mimata H, Moriyama M.
  • 雑誌名: Cancer Sci. 巻: 103(3) ページ: 569-76
• [雑誌論文] Downregulation of SAV1 plays a role in pathogenesis of high-grade clear cell renal cell carcinoma. (2011)
  • 著者名/発表者名: Matsuura K, Nakada C, Mashio M, Narimatsu T, Yoshimoto T, Tanigawa M, Tsukamoto Y, Hijiya N, Takeuchi I, Nomura T, Sato F, Mimata H, Seto M, Moriyama M.
  • 雑誌名: BMC Cancer 巻: 11 ページ: 523
• [雑誌論文] Identification of FOXO3 and PRDM1 as tumor-suppressor gene candidates in NK-cell neoplasms by genomic and functional analyses. (2011)
  • 著者名/発表者名: Karube K, Nakagawa M, Tsuzuki S, Takeuchi I, Honma K, Nakashima Y, Shimizu N, Ko YH, Morishima Y, Ohshima K, Nakamura S, Seto M.
  • 雑誌名: Blood. 巻: 118(12) ページ: 3195-204
• [雑誌論文] A phase II study of biweekly mitomycin C and irinotecan combination therapy in patients with fluoropyrimidine-resistant advanced gastric cancer: a report from the Gastrointestinal Oncology Group of the Japan Clinical Oncology Group (JCOG0109-DI Trial). (2011)
  • 著者名/発表者名: Hamaguchi T, Shirao K, Ohtsu A, Hyodo I, Arai Y, Takiuchi H, Fujii H, Yoshida M, Saito H, Denda T, Koizumi W, Iwase H, Boku N; Gastrointestinal Oncology Study Group of Japan Clinical Oncology Group.
  • 雑誌名: Gastric Cancer. 巻: 14(3) ページ: 226-33
• [雑誌論文] Aberrant DNA methylation status of endometriosis: epigenetics as the pathogenesis, biomarker and therapeutic target. (2011)
  • 著者名/発表者名: Nasu K, Kawano Y, Tsukamoto Y, Takano M, Takai N, Li H, Furukawa Y, Abe W, Moriyama M, Narahara H.
  • 雑誌名: J Obstet Gynaecol Res. 巻: 37(7) ページ: 683-95
• [雑誌論文] Genomic profiling of submucosal-invasive gastric cancer by array-based comparative genomic hybridization. (2011)
  • 著者名/発表者名: Kuroda A, Tsukamoto Y, Nguyen LT, Noguchi T, Takeuchi I, Uchida M, Uchida T, Hijiya N, Nakada C, Okimoto T, Kodama M, Murakami K, Matsuura K, Seto M, Ito H, Fujioka T, Moriyama M.
  • 雑誌名: PLoS One. 巻: 6(7) ページ: e22313
• [雑誌論文] Overexpression of miR-210, a downstream target of HIF1α, causes centrosome amplification in renal carcinoma cells. (2011)
  • 著者名/発表者名: Nakada C, Tsukamoto Y, Matsuura K, Nguyen TL, Hijiya N, Uchida T, Sato F, Mimata H, Seto M, Moriyama M.
  • 雑誌名: J Pathol. 巻: 224(2) ページ: 280-8
• [雑誌論文] Novel target genes responsive to the anti-growth activity of triptolide in endometrial and ovarian cancer cells. (2010)
  • 著者名/発表者名: Li H, Takai N, Yuge A, Furukawa Y, Tsuno A, Tsukamoto Y, Kong S, Moriyama M, Narahara H.
  • 雑誌名: Cancer Lett. 巻: 297(2) ページ: 198-206
• [雑誌論文] Vesicocutaneous fistula formation during treatment with sunitinib malate: Case report. (2010)
  • 著者名/発表者名: Watanabe K, Otsu S, Morinaga R, Kawano S, Hirashima Y, Sakashita H, Shirao K.
  • 雑誌名: BMC Gastroenterol 巻: 10 ページ: 128
• [雑誌論文] Second-line chemotherapy with irinotecan plus cisplatin after the failure of S-1 monotherapy for advanced gastric cancer. (2010)
  • 著者名/発表者名: Takahari D, Shimada Y, Takeshita S, Nishitani H, Takashima A, Okita N, Hirashima Y, Kato K, Hamaguchi T, Yamada Y, Shirao K.
  • 雑誌名: Gastric Cancer 巻: 13(3) ページ: 186-90
• [雑誌論文] Genomic profiling of gastric carcinoma in situ and adenomas by array-based comparative genomic hybridization. (2010)
  • 著者名/発表者名: Uchida M, Tsukamoto Y, Uchida T, Ishikawa Y, Nagai T, Hijiya N, Nguyen LT, Nakada C, Kuroda A, Okimoto T, Kodama M, Murakami K, Noguchi T, Matsuura K, Tanigawa M, Seto M, Ito H, Fujioka T, Takeuchi I, Moriyama M.
  • 雑誌名: J Pathol. 巻: 221(1) ページ: 96-105
• [雑誌論文] MicroRNA-375 is downregulated in gastric carcinomas and regulates cell survival by targeting PDK1 and 14-3-3zeta. (2010)
  • 著者名/発表者名: Tsukamoto Y, Nakada C, Noguchi T, Tanigawa M, Nguyen LT, Uchida T, Hijiya N, Matsuura K, Fujioka T, Seto M, Moriyama M.
  • 雑誌名: Cancer Res. 巻: 70(6) ページ: 2339-49
• [雑誌論文] Analysis of p53 mutations and the expression of p53 and p21WAF1/CIP1 protein in 15 cases of sebaceous carcinoma of the eyelid. (2010)
  • 著者名/発表者名: Kiyosaki K, Nakada C, Hijiya N, Tsukamoto Y, Matsuura K, Nakatsuka K, Daa T, Yokoyama S, Imaizumi M, Moriyama M.
  • 雑誌名: Invest Ophthalmol Vis Sci. 巻: 51(1) ページ: 7-11
• [雑誌論文] Analysis of virulence factors of Helicobacter pylori isolated from a Vietnamese population. (2009)
  • 著者名/発表者名: Uchida T, Nguyen LT, Takayama A, Okimoto T, Kodama M, Murakami K, Matsuhisa T, Trinh TD, Ta L, Ho DQ, Hoang HH, Kishida T, Fujioka T, Moriyama M, Yamaoka Y.
  • 雑誌名: BMC Microbiol. 巻: 9 ページ: 175
• [雑誌論文] The potential of copy number gains and losses, detected by array-based comparative genomic hybridization, for computational differential diagnosis of B-cell lymphomas and genetic regions involved in lymphomagenesis. (2009)
  • 著者名/発表者名: Takeuchi I, Tagawa H, Tsujikawa A, Nakagawa M, Katayama-Suguro M, Guo Y, Seto M.
  • 雑誌名: Haematologica 巻: 94(1) ページ: 61-9
• [雑誌論文] Array comparative genomic hybridization analysis of PTCL-U reveals a distinct subgroup with genetic alterations similar to lymphoma-type adult T-cell leukemia/lymphoma. (2009)
  • 著者名/発表者名: Nakagawa M, Nakagawa-Oshiro A, Karnan S, Tagawa H, Utsunomiya A, Nakamura S, Takeuchi I, Ohshima K, Seto M.
  • 雑誌名: Clin Cancer Res. 巻: 15(1) ページ: 30-8