ナノサージェリシステム構築を基盤とした高速バイオセンシング技術の開発

2013 年度 研究成果報告書

• 研究課題/領域番号: 23246044
• 研究種目: 基盤研究(A)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: 知能機械学・機械システム
• 研究機関: 名城大学 (2013); 名古屋大学 (2011-2012)
• 研究代表者: 福田 敏男 名城大学, 理工学部, 教授 (70156785)
• 研究分担者: 中島 正博 名古屋大学, 工学研究科, 助教 (80377837)
• 研究期間 (年度): 2011-05-31 – 2014-03-31
• キーワード: ナノサージェリ / バイオセンシング / マイクロ・ナノデバイス / モデル生物 / マイクロ流体デバイス

研究概要

現在，生物を直接利用することにより，安心・安全・高感度に環境物質を解析し，同時に生物への影響を解析するバイオセンシング技術が注目を集めている．本研究では，これまで構築してきたナノマニピュレーションシステムを応用し，新たな各種ナノツールを用いて，単一細胞レベルの局所操作及び計測技術を開拓した．また，マイクロ流体チップによりモデル生物を選択的に解析することで，重金属のバイオインジケータデバイスとして新たな応用を開拓した．

研究成果

• [雑誌論文] A microfluidic device with multi valves system to enable simultaneous several exposure tests on Caenorhabditis elegans (2014)
  • 著者名/発表者名: Jaehoon Jung, Masahiro Nakajima, Masaru Takeuchi, Qiang Huang, Toshio Fukuda
  • 雑誌名: Journal of Micromechanics and Microengineering 巻: Vol.24, No.3 ページ: 035012
  • 査読あり
• [雑誌論文] Microfluidic device for the continuous culture and analysis of Caenorhabditis elegans in a toxic aqueous environment (2013)
  • 著者名/発表者名: Jaehoon Jung, Masahiro Nakajima, Hirotaka Tajima, Toshio Fukuda
  • 雑誌名: Journal of Micromechatronics and Microengineering 巻: Vol.23 ページ: 085008, 8
  • 査読あり
• [雑誌論文] Single cell stiffness measurement at various humidity conditions by nanomanipulation of a nano-needle (2013)
  • 著者名/発表者名: Yajing Shen, Masahiro Nakajima, Zhan Yang, Hirotaka Tajima, Zoran Najdovski, Michio Homma, Toshio Fukuda
  • 雑誌名: Nanotechnology 巻: Vol.24, Number 14 ページ: 145703
  • 査読あり
• [雑誌論文] Instantaneous and Quantitative Single Cells Viability Determination Using Dual Nanoprobe Inside ESEM (2012)
  • 著者名/発表者名: Mohd Ridzuan Ahmad, Masahiro Nakajima, Masaru Kojima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • 雑誌名: IEEE Transactions on Nanotechnology 巻: Vol.11, No.2 ページ: 298-306
  • 査読あり
• [雑誌論文] Nanofork for Single Cells Adhesion Measurement via ESEM-Nanomanipulator System (2012)
  • 著者名/発表者名: Mohd Ridzuan Ahmad, Masahiro Nakajima, Masaru Kojima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • 雑誌名: IEEE Transactions on Nanobioscience 巻: Vol.11, No.1 ページ: 70-78
  • 査読あり
• [雑誌論文] Microchip device for measurement of body volume of C. elegans as bioindicator application (2012)
  • 著者名/発表者名: Jaehoon Jung, Masahiro Nakajima, Masaru Kojima, Katsutoshi Ooe, Toshio Fukuda
  • 雑誌名: Journal of Micro - Nano Mechatronics 巻: Vol.7 ページ: 3-11
  • 査読あり
• [雑誌論文] Evaluation of the single yeast cell's adhesion to ITO substrates with various surface energies via ESEM nanorobotic manipulation system (2011)
  • 著者名/発表者名: Yajing Shen, Mohd Ridzuan Ahmad, Masahiro Nakajima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • 雑誌名: IEEE Transactions on Nanobioscience 巻: Vol.10, No.4 ページ: 217-224
  • 査読あり
• [雑誌論文] Study of the time effect on the strength of cell-cell adhesion force by a novel nano-picker (2011)
  • 著者名/発表者名: Yajing Shen, Masahiro Nakajima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • 雑誌名: Biochemical and Biophysical Research Communication 巻: Vol.409, No.2 ページ: 160-165
  • 査読あり
• [雑誌論文] Single cell adhesion force measurement for cell viability identification by using AFM cantilever based micro putter (2011)
  • 著者名/発表者名: Yajing Shen, Masahiro Nakajima, Seiji Kojima, Michio Homma, Masaru Kojima, Toshio Fukuda
  • 雑誌名: Measurement science and technology 巻: Vol.22, No.11 ページ: 115802
  • 査読あり
• [雑誌論文] Design and characterization of nanoknife with buffering beam for in situ single cell cutting (2011)
  • 著者名/発表者名: Yajing Shen, Masahiro Nakajima, Zhan Yang, Seiji Kojima, Michio Homma, Toshio Fukuda
  • 雑誌名: Nanotechnology 巻: Vol.22, No.30 ページ: 305701
  • 査読あり
• [雑誌論文] Effect of ambient humidity on the strength of the adhesion force of single yeast cell inside environmental-SEM (2011)
  • 著者名/発表者名: Yajing Shen, Masahiro Nakajima, Mohd Ridzuan Ahmad, Seiji Kojima, Michio Homma, Toshio Fukuda
  • 雑誌名: Ultramicroscopy 巻: Vol.111, No.8 ページ: 1176-1183
  • 査読あり
• [雑誌論文] Buckling Nanoneedle for Characterizing Single Cells Mechanics inside Environmental SEM (2011)
  • 著者名/発表者名: Mohd Ridzuan Ahmad, Masahiro Nakajima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • 雑誌名: IEEE Transactions on Nanotechnology 巻: Vol.10 Issue 2 ページ: 226-236
  • 査読あり
• [学会発表] Microfluidic device for Coincident Multi-exposure test on C. elegans (2013)
  • 著者名/発表者名: Jahoon Jung, Masahiro Nakajima, Masaru Takeuchi, Qiang Huang, Toshio Fukuda
  • 学会等名: Proceedings 2013 International Symposium on Micromechatronics and Human Science
  • 発表場所: (60-63, 査読有)
  • 年月日: 20130000
• [学会発表] Real-Time Measurement of the Adhesion Force by Hybrid System of ESEM and AFM Cantilever (2013)
  • 著者名/発表者名: Yajing Shen, Masahiro Nakajima, Zhan Yang, Qiang Huang, Toshio Fukuda
  • 学会等名: Proceedings 13th IEEE International Conference on Nanotechnology(IEEE-Nano 2013)
  • 発表場所: (325-328, 査読有)
  • 年月日: 20130000
• [学会発表] Micro Fluidic Device to Analyze the Effect of Cadmium on Caenorhabditis elegans (2012)
  • 著者名/発表者名: Jaehoon Jung, Masahiro Nakajima, Hirotaka Tajima, Toshio Fukuda
  • 学会等名: Proceedings of the 2012 International Symposium on Micromechatronics and Human Science (MHS 2012)
  • 発表場所: (262-267, 査読有)
  • 年月日: 20120000
• [学会発表] Auto Nanomanipulation System for Single Cell Mechanical Property Characterization inside an Environmental SEM (2012)
  • 著者名/発表者名: Yajing Shen, Masahiro Nakajima, Masaru Kojima, Toshio Fukuda
  • 学会等名: Proceedings of the 2012 IEEE/RSJ International Conference on Intelligent Robotics and Systems (IROS 2012)
  • 発表場所: (646-651, 査読有)
  • 年月日: 20120000
• [学会発表] Micro Fluidic Device to Control the Position and to Analyze the Condition of C. elegans as a Bioindicator (2012)
  • 著者名/発表者名: Jaehoon Jung, Masahiro Nakajima, Masaru Kojima, Hirotaka Tajima, Toshio Fukuda
  • 学会等名: Proceedings of the 2012 IEEE/RSJ International Conference on Intelligent Robotics and Systems (IROS 2012)
  • 発表場所: (932-937, 査読有)
  • 年月日: 20120000
• [学会発表] Nano Needle with Buffering Beam for Single Cell Stiffness Measurement by Nanorobotic Manipulators inside ESEM (2012)
  • 著者名/発表者名: Yajing Shen, Masahiro Nakajima, Zhan Yang, Michio Homma, Toshio Fukuda
  • 学会等名: Proceedings of the 2012 12th IEEE Conference on Nanotechnology (IEEE-Nano 2012)
  • 発表場所: (4 pages, 1-4, 査読有)
  • 年月日: 20120000
• [学会発表] Development of the Auto Manipulation System towards the Single Cell Automatic Analysis inside an Environmental SEM (2012)
  • 著者名/発表者名: Yajing Shen, Masahiro Nakajima, TaoYue, Seiji Kojima, Michio Homma, Toshio Fukuda
  • 学会等名: Proceedings of the 2012 IEEE International Conference on Robotics and Automation (ICRA 2012)
  • 発表場所: (4594-4599, 査読有)
  • 年月日: 20120000
• [学会発表] Selective Nano-injection using Nano-Probe based on Nanomanipulation under Hybrid Microscope (2011)
  • 著者名/発表者名: Takahiro Hirano, Masahiro Nakajima, Masaru Kojima, Naoki Hisamoto, Michio Homma, Toshio Fukuda
  • 学会等名: Proceedings of the 2011 International Symposium on Micromechatronics and Human Science (MHS 2011)
  • 発表場所: (216-221, 査読有)
  • 年月日: 20110000
• [学会発表] Method to Study the Single Cell's Time-variation Adhesion Strength during the Manipulation inside ESEM (2011)
  • 著者名/発表者名: Yajing Shen, Masahiro Nakajima, Zoran Najdovski, Zhan Yang, Masaru Kojima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • 学会等名: Proceedings of the 2011 International Symposium on Micromechatronics and Human Science (MHS 2011)
  • 発表場所: (210-215, 査読有)
  • 年月日: 20110000
• [学会発表] Measurement of Body Volume of Live C. elegans by Microchip (2011)
  • 著者名/発表者名: Jaehoon Jung, Masahiro Nakajima, Masaru Kojima, Toshio Fukuda
  • 学会等名: Proceedings of the 2011 International Symposium on Micromechatronics and Human Science (MHS 2011)
  • 発表場所: (204-209, 査読有)
  • 年月日: 20110000
• [学会発表] Microchip Analysis Device for Fixed Caenorhabditis elegans by Tapered Micro channel (2011)
  • 著者名/発表者名: Jaehoon Jung, Masahiro Nakajima, Masaru Kojima, Toshio Fukuda
  • 学会等名: Proceedings of the SICE Annual Conference 2011
  • 発表場所: (2421-2426, 査読有)
  • 年月日: 20110000
• [学会発表] Evaluation of Nano-Knife's Edge Angle for Single Cell Cutting by Using Nanorobotic Manipulators Inside ESEM (2011)
  • 著者名/発表者名: Yajing Shen, Masahiro Nakajima, Zhang Yang, Seiji Kojima, Michio Homma, Toshio Fukuda, Masaru Kojima
  • 学会等名: Proceedings of the 2011 11th IEEE Conf. on Nanotechnology (IEEE-Nano 2011)
  • 発表場所: (Vol.1, 155-160, 査読有)
  • 年月日: 20110000
• [学会発表] Direct Nano-injection Method by Nanoprobe Insertion based on E-SEM Nanorobotic Manipulation under Hybrid Microscope (2011)
  • 著者名/発表者名: Masahiro Nakajima, Takanori Hirano, Masaru Kojima, Naoki Hisamoto, Michio Homma, Toshio Fukuda
  • 学会等名: Proceedings of the 2011 IEEE International Conference on Robotics and Automation (ICRA 2011)
  • 発表場所: (4139-4144, 査読有)
  • 年月日: 20110000
• [学会発表] Characterization of Oscillating Nano Knife for Single Cell Cutting by Nanorobotic Manipulation System inside ESEM (2011)
  • 著者名/発表者名: Yajing Shen, Masahiro Nakajima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • 学会等名: Proceedings of the 2011 IEEE International Conference on Robotics and Automation (ICRA 2011)
  • 発表場所: (4133-4138, 査読有)
  • 年月日: 20110000
• [学会発表] Measurement for Viability Identification using Nanorobotic Manipulation System inside ESEM (2011)
  • 著者名/発表者名: Yajing Shen, Masahiro Nakajima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • 学会等名: Proceedings of 2011 IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2011)
  • 発表場所: (944-947, 査読有)
  • 年月日: 20110000
• [備考]
  • URL: http://www.mein.nagoya-u.ac.jp/indexj.html
• [産業財産権] 微生物の電気化学的特性の解析用マイクロチップデバイス (2011)
  • 発明者名: 福田敏男,中島正博,小嶋勝
  • 権利者名: 福田敏男,中島正博,小嶋勝
  • 産業財産権番号: 特願2011-231134
  • 出願年月日: 2011-09-16